Prolonged DNA hydrolysis in water: A study on DNA stability

This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0–36 h. The chemical/physical features of the resulting samples are desc...

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Detalles Bibliográficos
Autores principales: Fattorini, Paolo, Marrubini, Giorgio, Bonin, Serena, Bertoglio, Barbara, Grignani, Pierangela, Recchia, Elisa, Pitacco, Paola, Procopio, Francesca, Cantoni, Carolina, Pajnič, Irena Zupanič, Sorçaburu-Cigliero, Solange, Previdere`, Carlo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Physics and Astronomy
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143716/
https://www.ncbi.nlm.nih.gov/pubmed/30238033
http://dx.doi.org/10.1016/j.dib.2018.08.120
Descripción
Sumario:This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0–36 h. The chemical/physical features of the resulting samples are described. After normalization of the qPCR data, these were compared with those obtained from samples treated for 0–10 h in a previous study.

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