Prolonged DNA hydrolysis in water: A study on DNA stability

This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0–36 h. The chemical/physical features of the resulting samples are desc...

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Detalles Bibliográficos
Autores principales: Fattorini, Paolo, Marrubini, Giorgio, Bonin, Serena, Bertoglio, Barbara, Grignani, Pierangela, Recchia, Elisa, Pitacco, Paola, Procopio, Francesca, Cantoni, Carolina, Pajnič, Irena Zupanič, Sorçaburu-Cigliero, Solange, Previdere`, Carlo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Physics and Astronomy
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143716/
https://www.ncbi.nlm.nih.gov/pubmed/30238033
http://dx.doi.org/10.1016/j.dib.2018.08.120
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author Fattorini, Paolo
Marrubini, Giorgio
Bonin, Serena
Bertoglio, Barbara
Grignani, Pierangela
Recchia, Elisa
Pitacco, Paola
Procopio, Francesca
Cantoni, Carolina
Pajnič, Irena Zupanič
Sorçaburu-Cigliero, Solange
Previdere`, Carlo
author_facet Fattorini, Paolo
Marrubini, Giorgio
Bonin, Serena
Bertoglio, Barbara
Grignani, Pierangela
Recchia, Elisa
Pitacco, Paola
Procopio, Francesca
Cantoni, Carolina
Pajnič, Irena Zupanič
Sorçaburu-Cigliero, Solange
Previdere`, Carlo
author_sort Fattorini, Paolo
collection PubMed
description This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0–36 h. The chemical/physical features of the resulting samples are described. After normalization of the qPCR data, these were compared with those obtained from samples treated for 0–10 h in a previous study.
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spelling pubmed-61437162018-09-20 Prolonged DNA hydrolysis in water: A study on DNA stability Fattorini, Paolo Marrubini, Giorgio Bonin, Serena Bertoglio, Barbara Grignani, Pierangela Recchia, Elisa Pitacco, Paola Procopio, Francesca Cantoni, Carolina Pajnič, Irena Zupanič Sorçaburu-Cigliero, Solange Previdere`, Carlo Data Brief Physics and Astronomy This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0–36 h. The chemical/physical features of the resulting samples are described. After normalization of the qPCR data, these were compared with those obtained from samples treated for 0–10 h in a previous study. Elsevier 2018-08-30 /pmc/articles/PMC6143716/ /pubmed/30238033 http://dx.doi.org/10.1016/j.dib.2018.08.120 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Physics and Astronomy
Fattorini, Paolo
Marrubini, Giorgio
Bonin, Serena
Bertoglio, Barbara
Grignani, Pierangela
Recchia, Elisa
Pitacco, Paola
Procopio, Francesca
Cantoni, Carolina
Pajnič, Irena Zupanič
Sorçaburu-Cigliero, Solange
Previdere`, Carlo
Prolonged DNA hydrolysis in water: A study on DNA stability
title Prolonged DNA hydrolysis in water: A study on DNA stability
title_full Prolonged DNA hydrolysis in water: A study on DNA stability
title_fullStr Prolonged DNA hydrolysis in water: A study on DNA stability
title_full_unstemmed Prolonged DNA hydrolysis in water: A study on DNA stability
title_short Prolonged DNA hydrolysis in water: A study on DNA stability
title_sort prolonged dna hydrolysis in water: a study on dna stability
topic Physics and Astronomy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143716/
https://www.ncbi.nlm.nih.gov/pubmed/30238033
http://dx.doi.org/10.1016/j.dib.2018.08.120
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