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Prolonged DNA hydrolysis in water: A study on DNA stability
This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0–36 h. The chemical/physical features of the resulting samples are desc...
Autores principales: | Fattorini, Paolo, Marrubini, Giorgio, Bonin, Serena, Bertoglio, Barbara, Grignani, Pierangela, Recchia, Elisa, Pitacco, Paola, Procopio, Francesca, Cantoni, Carolina, Pajnič, Irena Zupanič, Sorçaburu-Cigliero, Solange, Previdere`, Carlo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143716/ https://www.ncbi.nlm.nih.gov/pubmed/30238033 http://dx.doi.org/10.1016/j.dib.2018.08.120 |
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