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New Plasmids for Fusarium Transformation Allowing Positive-Negative Selection and Efficient Cre-loxP Mediated Marker Recycling
In filamentous fungi such as Fusarium graminearum, disruption of multiple genes of interest in the same strain (e.g., to test for redundant gene function) is a difficult task due to the limited availability of reliable selection markers. We have created a series of transformation vectors that allow...
Autores principales: | Twaruschek, Krisztian, Spörhase, Pia, Michlmayr, Herbert, Wiesenberger, Gerlinde, Adam, Gerhard |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143793/ https://www.ncbi.nlm.nih.gov/pubmed/30258410 http://dx.doi.org/10.3389/fmicb.2018.01954 |
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