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Expression levels of IL-15 and IL-17 in synovial fluid of rheumatoid arthritis animal model

The aim of the present study was to investigate the expression levels of interleukin-15 (IL-15) and interleukin-17 (IL-17) in synovial fluid of rheumatoid arthritis (RA) animal model, and to investigate their correlations with RA. A total of 100 Wistar rats were selected, among which 60 rats were us...

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Autores principales: Jin, Yi, Chen, Xiao, Gao, Zongyan, Liu, Ke, Hou, Yi, Zheng, Jia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143829/
https://www.ncbi.nlm.nih.gov/pubmed/30233684
http://dx.doi.org/10.3892/etm.2018.6643
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author Jin, Yi
Chen, Xiao
Gao, Zongyan
Liu, Ke
Hou, Yi
Zheng, Jia
author_facet Jin, Yi
Chen, Xiao
Gao, Zongyan
Liu, Ke
Hou, Yi
Zheng, Jia
author_sort Jin, Yi
collection PubMed
description The aim of the present study was to investigate the expression levels of interleukin-15 (IL-15) and interleukin-17 (IL-17) in synovial fluid of rheumatoid arthritis (RA) animal model, and to investigate their correlations with RA. A total of 100 Wistar rats were selected, among which 60 rats were used to establish the collagen II-induced arthritis (CIA) model as the model observation group, and the remaining 40 rats were used as blank control group. The levels of IL-15 and IL-17 in synovial fluid were detected via enzyme-linked immunosorbent assay (ELISA) at 1, 7, 14, 21 and 28 days after successful modeling. RA was evaluated by using arthritis index (AI) and pedal swelling volume. The expression levels of IL-15 and IL-17 in synovial fluid of rats in model observation group were higher than those in blank control group (P<0.05), and the levels of IL-15 and IL-17 in model observation group were gradually increased over time. In model observation group at 7 days after modeling, AI and pedal swelling volume began to be increased gradually reaching a peak at 28 days. The pedal swelling volume of CIA model rats was significantly higher than that of the blank control group (P<0.05). The increased expression levels of IL-15 and IL-17 in synovial fluid of rats in the CIA model observation group are correlated with the activity of disease, which can be used as reference indexes for the activity of RA.
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spelling pubmed-61438292018-09-19 Expression levels of IL-15 and IL-17 in synovial fluid of rheumatoid arthritis animal model Jin, Yi Chen, Xiao Gao, Zongyan Liu, Ke Hou, Yi Zheng, Jia Exp Ther Med Articles The aim of the present study was to investigate the expression levels of interleukin-15 (IL-15) and interleukin-17 (IL-17) in synovial fluid of rheumatoid arthritis (RA) animal model, and to investigate their correlations with RA. A total of 100 Wistar rats were selected, among which 60 rats were used to establish the collagen II-induced arthritis (CIA) model as the model observation group, and the remaining 40 rats were used as blank control group. The levels of IL-15 and IL-17 in synovial fluid were detected via enzyme-linked immunosorbent assay (ELISA) at 1, 7, 14, 21 and 28 days after successful modeling. RA was evaluated by using arthritis index (AI) and pedal swelling volume. The expression levels of IL-15 and IL-17 in synovial fluid of rats in model observation group were higher than those in blank control group (P<0.05), and the levels of IL-15 and IL-17 in model observation group were gradually increased over time. In model observation group at 7 days after modeling, AI and pedal swelling volume began to be increased gradually reaching a peak at 28 days. The pedal swelling volume of CIA model rats was significantly higher than that of the blank control group (P<0.05). The increased expression levels of IL-15 and IL-17 in synovial fluid of rats in the CIA model observation group are correlated with the activity of disease, which can be used as reference indexes for the activity of RA. D.A. Spandidos 2018-10 2018-08-22 /pmc/articles/PMC6143829/ /pubmed/30233684 http://dx.doi.org/10.3892/etm.2018.6643 Text en Copyright: © Jin et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Jin, Yi
Chen, Xiao
Gao, Zongyan
Liu, Ke
Hou, Yi
Zheng, Jia
Expression levels of IL-15 and IL-17 in synovial fluid of rheumatoid arthritis animal model
title Expression levels of IL-15 and IL-17 in synovial fluid of rheumatoid arthritis animal model
title_full Expression levels of IL-15 and IL-17 in synovial fluid of rheumatoid arthritis animal model
title_fullStr Expression levels of IL-15 and IL-17 in synovial fluid of rheumatoid arthritis animal model
title_full_unstemmed Expression levels of IL-15 and IL-17 in synovial fluid of rheumatoid arthritis animal model
title_short Expression levels of IL-15 and IL-17 in synovial fluid of rheumatoid arthritis animal model
title_sort expression levels of il-15 and il-17 in synovial fluid of rheumatoid arthritis animal model
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143829/
https://www.ncbi.nlm.nih.gov/pubmed/30233684
http://dx.doi.org/10.3892/etm.2018.6643
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