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Subarachnoid hemorrhage enhances the expression of TDP-43 in the brain of experimental rats and human subjects

The transactive response DNA-binding protein of 43 (TDP-43) may be involved in neurodegenerative disease and in the response to brain injury; however, alterations in the expression of TDP-43 following subarachnoid hemorrhage (SAH) require further investigation. The present study reported a notable e...

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Autores principales: He, Tibiao, Zuo, Yuchun, Ai-Zakwani, Kauthar, Luo, Jing, Zhu, Haixia, Yan, Xiao-Xin, Liu, Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143865/
https://www.ncbi.nlm.nih.gov/pubmed/30233682
http://dx.doi.org/10.3892/etm.2018.6636
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author He, Tibiao
Zuo, Yuchun
Ai-Zakwani, Kauthar
Luo, Jing
Zhu, Haixia
Yan, Xiao-Xin
Liu, Fei
author_facet He, Tibiao
Zuo, Yuchun
Ai-Zakwani, Kauthar
Luo, Jing
Zhu, Haixia
Yan, Xiao-Xin
Liu, Fei
author_sort He, Tibiao
collection PubMed
description The transactive response DNA-binding protein of 43 (TDP-43) may be involved in neurodegenerative disease and in the response to brain injury; however, alterations in the expression of TDP-43 following subarachnoid hemorrhage (SAH) require further investigation. The present study reported a notable elevation in the expression of TDP-43 within the cerebrospinal fluid (CSF) of patients with aneurysmal SAH and increased brain expression of TDP-43 in a rat model of SAH. The TDP-43 protein and a derivative migrated at 43 and 24 kDa, respectively, as observed via the immunoblotting of concentrated CSF samples obtained from patients with SAH; no signal was detected in the CSF from healthy controls. SAH in rats was induced by intravascular suture puncture. The expression levels of TDP-43 in rat cortical lysates following SAH were increased at 0.5 h, peaked at 48 h and remained significantly elevated at 72 h post-injury, compared with sham controls. TDP-43 immunolabeling indication localization within neurons, astrocytes and microglia in the experimental rats. Collectively, the findings of the present study indicated the early involvement of TDP-43 in the brain in response to SAH, and that expression levels of TDP-43 in the CSF may serve as a prognostic biomarker among patients with this condition.
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spelling pubmed-61438652018-09-19 Subarachnoid hemorrhage enhances the expression of TDP-43 in the brain of experimental rats and human subjects He, Tibiao Zuo, Yuchun Ai-Zakwani, Kauthar Luo, Jing Zhu, Haixia Yan, Xiao-Xin Liu, Fei Exp Ther Med Articles The transactive response DNA-binding protein of 43 (TDP-43) may be involved in neurodegenerative disease and in the response to brain injury; however, alterations in the expression of TDP-43 following subarachnoid hemorrhage (SAH) require further investigation. The present study reported a notable elevation in the expression of TDP-43 within the cerebrospinal fluid (CSF) of patients with aneurysmal SAH and increased brain expression of TDP-43 in a rat model of SAH. The TDP-43 protein and a derivative migrated at 43 and 24 kDa, respectively, as observed via the immunoblotting of concentrated CSF samples obtained from patients with SAH; no signal was detected in the CSF from healthy controls. SAH in rats was induced by intravascular suture puncture. The expression levels of TDP-43 in rat cortical lysates following SAH were increased at 0.5 h, peaked at 48 h and remained significantly elevated at 72 h post-injury, compared with sham controls. TDP-43 immunolabeling indication localization within neurons, astrocytes and microglia in the experimental rats. Collectively, the findings of the present study indicated the early involvement of TDP-43 in the brain in response to SAH, and that expression levels of TDP-43 in the CSF may serve as a prognostic biomarker among patients with this condition. D.A. Spandidos 2018-10 2018-08-21 /pmc/articles/PMC6143865/ /pubmed/30233682 http://dx.doi.org/10.3892/etm.2018.6636 Text en Copyright: © He et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
He, Tibiao
Zuo, Yuchun
Ai-Zakwani, Kauthar
Luo, Jing
Zhu, Haixia
Yan, Xiao-Xin
Liu, Fei
Subarachnoid hemorrhage enhances the expression of TDP-43 in the brain of experimental rats and human subjects
title Subarachnoid hemorrhage enhances the expression of TDP-43 in the brain of experimental rats and human subjects
title_full Subarachnoid hemorrhage enhances the expression of TDP-43 in the brain of experimental rats and human subjects
title_fullStr Subarachnoid hemorrhage enhances the expression of TDP-43 in the brain of experimental rats and human subjects
title_full_unstemmed Subarachnoid hemorrhage enhances the expression of TDP-43 in the brain of experimental rats and human subjects
title_short Subarachnoid hemorrhage enhances the expression of TDP-43 in the brain of experimental rats and human subjects
title_sort subarachnoid hemorrhage enhances the expression of tdp-43 in the brain of experimental rats and human subjects
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143865/
https://www.ncbi.nlm.nih.gov/pubmed/30233682
http://dx.doi.org/10.3892/etm.2018.6636
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