Cargando…

Structural basis for the synergy of 4′- and 2′-modifications on siRNA nuclease resistance, thermal stability and RNAi activity

Chemical modification is a prerequisite of oligonucleotide therapeutics for improved metabolic stability, uptake and activity, irrespective of their mode of action, i.e. antisense, RNAi or aptamer. Phosphate moiety and ribose C2′/O2′ atoms are the most common sites for modification. Compared to 2′-O...

Descripción completa

Detalles Bibliográficos
Autores principales:	Harp, Joel M, Guenther, Dale C, Bisbe, Anna, Perkins, Lydia, Matsuda, Shigeo, Bommineni, Gopal R, Zlatev, Ivan, Foster, Donald J, Taneja, Nate, Charisse, Klaus, Maier, Martin A, Rajeev, Kallanthottathil G, Manoharan, Muthiah, Egli, Martin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2018
Materias:	Chemical Biology and Nucleic Acid Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6144868/ https://www.ncbi.nlm.nih.gov/pubmed/30107495 http://dx.doi.org/10.1093/nar/gky703

Ejemplares similares

Chirality matters: stereo-defined phosphorothioate linkages at the termini of small interfering RNAs improve pharmacology in vivo
por: Jahns, Hartmut, et al.
Publicado: (2021)

Chimeric siRNAs with chemically modified pentofuranose and hexopyranose nucleotides: altritol-nucleotide (ANA) containing GalNAc–siRNA conjugates: in vitro and in vivo RNAi activity and resistance to 5′-exonuclease
por: Kumar, Pawan, et al.
Publicado: (2020)

Overcoming GNA/RNA base-pairing limitations using isonucleotides improves the pharmacodynamic activity of ESC+ GalNAc-siRNAs
por: Schlegel, Mark K, et al.
Publicado: (2021)

Synthesis, chirality-dependent conformational and biological properties of siRNAs containing 5′-(R)- and 5′-(S)-C-methyl-guanosine
por: Mikami, Atsushi, et al.
Publicado: (2020)

siRNAs containing 2′-fluorinated Northern-methanocarbacyclic (2′-F-NMC) nucleotides: in vitro and in vivo RNAi activity and inability of mitochondrial polymerases to incorporate 2′-F-NMC NTPs
por: Akabane-Nakata, Masaaki, et al.
Publicado: (2021)

Small circular interfering RNAs (sciRNAs) as a potent therapeutic platform for gene-silencing
por: Jahns, Hartmut, et al.
Publicado: (2021)

Safety evaluation of 2′-deoxy-2′-fluoro nucleotides in GalNAc-siRNA conjugates
por: Janas, Maja M, et al.
Publicado: (2019)

Impact of enhanced metabolic stability on pharmacokinetics and pharmacodynamics of GalNAc–siRNA conjugates
por: Nair, Jayaprakash K., et al.
Publicado: (2017)

Acyclic (S)-glycol nucleic acid (S-GNA) modification of siRNAs improves the safety of RNAi therapeutics while maintaining potency
por: Egli, Martin, et al.
Publicado: (2023)

From bench to bedside: Improving the clinical safety of GalNAc–siRNA conjugates using seed-pairing destabilization
por: Schlegel, Mark K, et al.
Publicado: (2022)

Molecular basis of cyclic tetra-oligoadenylate processing by small standalone CRISPR-Cas ring nucleases
por: Molina, Rafael, et al.
Publicado: (2022)

Tetrameric restriction enzymes: expansion to the GIY-YIG nuclease family
por: Gasiunas, Giedrius, et al.
Publicado: (2008)

A novel DNA nuclease is stimulated by association with the GINS complex
por: Li, Zhuo, et al.
Publicado: (2011)

Autonomous zinc-finger nuclease pairs for targeted chromosomal deletion
por: Şöllü, Cem, et al.
Publicado: (2010)

Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants
por: Tóth, Eszter, et al.
Publicado: (2018)

Function of the N-terminal segment of the RecA-dependent nuclease Ref
por: Gruber, Angela J., et al.
Publicado: (2015)

Dimerization of SLX4 contributes to functioning of the SLX4-nuclease complex
por: Yin, Jinhu, et al.
Publicado: (2016)

Regulation by interdomain communication of a headful packaging nuclease from bacteriophage T4
por: Ghosh-Kumar, Manjira, et al.
Publicado: (2011)

Dna2 is a structure-specific nuclease, with affinity for 5′-flap intermediates
por: Stewart, Jason A., et al.
Publicado: (2010)

The nuclease domain of the SPP1 packaging motor coordinates DNA cleavage and encapsidation
por: Cornilleau, Charlène, et al.
Publicado: (2013)

megaTALs: a rare-cleaving nuclease architecture for therapeutic genome engineering
por: Boissel, Sandrine, et al.
Publicado: (2014)

A programmable omnipotent Argonaute nuclease from mesophilic bacteria Kurthia massiliensis
por: Liu, Yang, et al.
Publicado: (2021)

Bacterial Argonaute nucleases reveal different modes of DNA targeting in vitro and in vivo
por: Lisitskaya, Lidiya, et al.
Publicado: (2023)

The AddAB helicase–nuclease catalyses rapid and processive DNA unwinding using a single Superfamily 1A motor domain
por: Yeeles, Joseph T.P., et al.
Publicado: (2011)

Mutational and biochemical analysis of the DNA-entry nuclease EndA from Streptococcus pneumoniae
por: Midon, Marika, et al.
Publicado: (2011)

Efficient processing of TFO-directed psoralen DNA interstrand crosslinks by the UvrABC nuclease
por: Christensen, Laura A., et al.
Publicado: (2008)

A novel zinc-finger nuclease platform with a sequence-specific cleavage module
por: Schierling, Benno, et al.
Publicado: (2012)

An Mrr-family nuclease motif in the single polypeptide restriction–modification enzyme LlaGI
por: Smith, Rachel M., et al.
Publicado: (2009)

Using defined finger–finger interfaces as units of assembly for constructing zinc-finger nucleases
por: Zhu, Cong, et al.
Publicado: (2013)

Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA
por: Swarts, Daan C., et al.
Publicado: (2015)

Creating highly specific nucleases by fusion of active restriction endonucleases and catalytically inactive homing endonucleases
por: Fonfara, Ines, et al.
Publicado: (2012)

Programmable DNA cleavage by Ago nucleases from mesophilic bacteria Clostridium butyricum and Limnothrix rosea
por: Kuzmenko, Anton, et al.
Publicado: (2019)

Small-molecule sensitization of RecBCD helicase–nuclease to a Chi hotspot-activated state
por: Karabulut, Ahmet C, et al.
Publicado: (2020)

Parallel dimerization of a PrrC-anticodon nuclease region implicated in tRNA(Lys) recognition
por: Klaiman, Daniel, et al.
Publicado: (2007)

A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity
por: Mussolino, Claudio, et al.
Publicado: (2011)

The trimeric Hef-associated nuclease HAN is a 3′→5′ exonuclease and is probably involved in DNA repair
por: Feng, Lei, et al.
Publicado: (2018)

Fuse to defuse: a self-limiting ribonuclease-ring nuclease fusion for type III CRISPR defence
por: Samolygo, Aleksei, et al.
Publicado: (2020)

DNA-mediated coupling of ATPase, translocase and nuclease activities of a Type ISP restriction-modification enzyme
por: Chand, Mahesh Kumar, et al.
Publicado: (2020)

Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs
por: Jacobsen, Thomas, et al.
Publicado: (2020)

High-fidelity KKH variant of Staphylococcus aureus Cas9 nucleases with improved base mismatch discrimination
por: Yuen, Chaya T L, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_al7t8iav1arfigv5pivfmfpa16