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A dynamic model of lignin biosynthesis in Brachypodium distachyon
BACKGROUND: Lignin is a crucial molecule for terrestrial plants, as it offers structural support and permits the transport of water over long distances. The hardness of lignin reduces plant digestibility by cattle and sheep; it also makes inedible plant materials recalcitrant toward the enzymatic fe...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6145374/ https://www.ncbi.nlm.nih.gov/pubmed/30250505 http://dx.doi.org/10.1186/s13068-018-1241-6 |
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author | Faraji, Mojdeh Fonseca, Luis L. Escamilla-Treviño, Luis Barros-Rios, Jaime Engle, Nancy L. Yang, Zamin K. Tschaplinski, Timothy J. Dixon, Richard A. Voit, Eberhard O. |
author_facet | Faraji, Mojdeh Fonseca, Luis L. Escamilla-Treviño, Luis Barros-Rios, Jaime Engle, Nancy L. Yang, Zamin K. Tschaplinski, Timothy J. Dixon, Richard A. Voit, Eberhard O. |
author_sort | Faraji, Mojdeh |
collection | PubMed |
description | BACKGROUND: Lignin is a crucial molecule for terrestrial plants, as it offers structural support and permits the transport of water over long distances. The hardness of lignin reduces plant digestibility by cattle and sheep; it also makes inedible plant materials recalcitrant toward the enzymatic fermentation of cellulose, which is a potentially valuable substrate for sustainable biofuels. Targeted attempts to change the amount or composition of lignin in relevant plant species have been hampered by the fact that the lignin biosynthetic pathway is difficult to understand, because it uses several enzymes for the same substrates, is regulated in an ill-characterized manner, may operate in different locations within cells, and contains metabolic channels, which the plant may use to funnel initial substrates into specific monolignols. RESULTS: We propose a dynamic mathematical model that integrates various datasets and other information regarding the lignin pathway in Brachypodium distachyon and permits explanations for some counterintuitive observations. The model predicts the lignin composition and label distribution in a BdPTAL knockdown strain, with results that are quite similar to experimental data. CONCLUSION: Given the present scarcity of available data, the model resulting from our analysis is presumably not final. However, it offers proof of concept for how one may design integrative pathway models of this type, which are necessary tools for predicting the consequences of genomic or other alterations toward plants with lignin features that are more desirable than in their wild-type counterparts. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13068-018-1241-6) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6145374 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-61453742018-09-24 A dynamic model of lignin biosynthesis in Brachypodium distachyon Faraji, Mojdeh Fonseca, Luis L. Escamilla-Treviño, Luis Barros-Rios, Jaime Engle, Nancy L. Yang, Zamin K. Tschaplinski, Timothy J. Dixon, Richard A. Voit, Eberhard O. Biotechnol Biofuels Research BACKGROUND: Lignin is a crucial molecule for terrestrial plants, as it offers structural support and permits the transport of water over long distances. The hardness of lignin reduces plant digestibility by cattle and sheep; it also makes inedible plant materials recalcitrant toward the enzymatic fermentation of cellulose, which is a potentially valuable substrate for sustainable biofuels. Targeted attempts to change the amount or composition of lignin in relevant plant species have been hampered by the fact that the lignin biosynthetic pathway is difficult to understand, because it uses several enzymes for the same substrates, is regulated in an ill-characterized manner, may operate in different locations within cells, and contains metabolic channels, which the plant may use to funnel initial substrates into specific monolignols. RESULTS: We propose a dynamic mathematical model that integrates various datasets and other information regarding the lignin pathway in Brachypodium distachyon and permits explanations for some counterintuitive observations. The model predicts the lignin composition and label distribution in a BdPTAL knockdown strain, with results that are quite similar to experimental data. CONCLUSION: Given the present scarcity of available data, the model resulting from our analysis is presumably not final. However, it offers proof of concept for how one may design integrative pathway models of this type, which are necessary tools for predicting the consequences of genomic or other alterations toward plants with lignin features that are more desirable than in their wild-type counterparts. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13068-018-1241-6) contains supplementary material, which is available to authorized users. BioMed Central 2018-09-19 /pmc/articles/PMC6145374/ /pubmed/30250505 http://dx.doi.org/10.1186/s13068-018-1241-6 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Faraji, Mojdeh Fonseca, Luis L. Escamilla-Treviño, Luis Barros-Rios, Jaime Engle, Nancy L. Yang, Zamin K. Tschaplinski, Timothy J. Dixon, Richard A. Voit, Eberhard O. A dynamic model of lignin biosynthesis in Brachypodium distachyon |
title | A dynamic model of lignin biosynthesis in Brachypodium distachyon |
title_full | A dynamic model of lignin biosynthesis in Brachypodium distachyon |
title_fullStr | A dynamic model of lignin biosynthesis in Brachypodium distachyon |
title_full_unstemmed | A dynamic model of lignin biosynthesis in Brachypodium distachyon |
title_short | A dynamic model of lignin biosynthesis in Brachypodium distachyon |
title_sort | dynamic model of lignin biosynthesis in brachypodium distachyon |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6145374/ https://www.ncbi.nlm.nih.gov/pubmed/30250505 http://dx.doi.org/10.1186/s13068-018-1241-6 |
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