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Subgingival microbiome of rheumatoid arthritis patients in relation to their disease status and periodontal health
OBJECTIVE: Rheumatoid arthritis (RA) and periodontitis are chronic inflammatory diseases that share common risk factors. However, the bidirectional relationship between RA and periodontal disease is not fully understood. This study was undertaken to describe the bacterial component of the subgingiva...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6145512/ https://www.ncbi.nlm.nih.gov/pubmed/30231060 http://dx.doi.org/10.1371/journal.pone.0202278 |
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author | Beyer, Kathrin Zaura, Egija Brandt, Bernd W. Buijs, Mark J. Brun, Johan G. Crielaard, Wim Bolstad, Anne Isine |
author_facet | Beyer, Kathrin Zaura, Egija Brandt, Bernd W. Buijs, Mark J. Brun, Johan G. Crielaard, Wim Bolstad, Anne Isine |
author_sort | Beyer, Kathrin |
collection | PubMed |
description | OBJECTIVE: Rheumatoid arthritis (RA) and periodontitis are chronic inflammatory diseases that share common risk factors. However, the bidirectional relationship between RA and periodontal disease is not fully understood. This study was undertaken to describe the bacterial component of the subgingival microbiome in RA patients and to relate this to RA disease activity and periodontal status. METHODS: Patients with chronic established RA (N = 78) were periodontally examined and their subgingival plaque samples were collected; their clinical and laboratory data on RA status and medication were obtained from medical records. Bacterial DNA was quantified by universal 16S rDNA qPCR, and Porphyromonas gingivalis by species-specific qPCR. For microbiome assessment, 16S rDNA amplicon sequencing was performed. RESULTS: Active RA was diagnosed in 58% of the patients and periodontitis in 82% (mild: 9%, moderate: 55%, severe: 18%). P. gingivalis was present in 14% of the samples. Different levels of gingival bleeding, periodontal probing depth, RA disease status, prednisolone use and smoking were associated with significantly different microbiome compositions. Two subgingival microbial community types were discerned. CONCLUSION: In RA patients with active disease, anti-inflammatory medication as part of RA therapy was associated with better oral health status and a healthier subgingival microbiome compared to that of RA patients in remission, especially those in remission who were current smokers. RA patients in remission with current smoking status may particularly benefit from a systematic periodontal treatment program. The potential role of microbial community types in patient stratification and personalized therapy should be assessed in longitudinal studies. |
format | Online Article Text |
id | pubmed-6145512 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-61455122018-10-08 Subgingival microbiome of rheumatoid arthritis patients in relation to their disease status and periodontal health Beyer, Kathrin Zaura, Egija Brandt, Bernd W. Buijs, Mark J. Brun, Johan G. Crielaard, Wim Bolstad, Anne Isine PLoS One Research Article OBJECTIVE: Rheumatoid arthritis (RA) and periodontitis are chronic inflammatory diseases that share common risk factors. However, the bidirectional relationship between RA and periodontal disease is not fully understood. This study was undertaken to describe the bacterial component of the subgingival microbiome in RA patients and to relate this to RA disease activity and periodontal status. METHODS: Patients with chronic established RA (N = 78) were periodontally examined and their subgingival plaque samples were collected; their clinical and laboratory data on RA status and medication were obtained from medical records. Bacterial DNA was quantified by universal 16S rDNA qPCR, and Porphyromonas gingivalis by species-specific qPCR. For microbiome assessment, 16S rDNA amplicon sequencing was performed. RESULTS: Active RA was diagnosed in 58% of the patients and periodontitis in 82% (mild: 9%, moderate: 55%, severe: 18%). P. gingivalis was present in 14% of the samples. Different levels of gingival bleeding, periodontal probing depth, RA disease status, prednisolone use and smoking were associated with significantly different microbiome compositions. Two subgingival microbial community types were discerned. CONCLUSION: In RA patients with active disease, anti-inflammatory medication as part of RA therapy was associated with better oral health status and a healthier subgingival microbiome compared to that of RA patients in remission, especially those in remission who were current smokers. RA patients in remission with current smoking status may particularly benefit from a systematic periodontal treatment program. The potential role of microbial community types in patient stratification and personalized therapy should be assessed in longitudinal studies. Public Library of Science 2018-09-19 /pmc/articles/PMC6145512/ /pubmed/30231060 http://dx.doi.org/10.1371/journal.pone.0202278 Text en © 2018 Beyer et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Beyer, Kathrin Zaura, Egija Brandt, Bernd W. Buijs, Mark J. Brun, Johan G. Crielaard, Wim Bolstad, Anne Isine Subgingival microbiome of rheumatoid arthritis patients in relation to their disease status and periodontal health |
title | Subgingival microbiome of rheumatoid arthritis patients in relation to their disease status and periodontal health |
title_full | Subgingival microbiome of rheumatoid arthritis patients in relation to their disease status and periodontal health |
title_fullStr | Subgingival microbiome of rheumatoid arthritis patients in relation to their disease status and periodontal health |
title_full_unstemmed | Subgingival microbiome of rheumatoid arthritis patients in relation to their disease status and periodontal health |
title_short | Subgingival microbiome of rheumatoid arthritis patients in relation to their disease status and periodontal health |
title_sort | subgingival microbiome of rheumatoid arthritis patients in relation to their disease status and periodontal health |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6145512/ https://www.ncbi.nlm.nih.gov/pubmed/30231060 http://dx.doi.org/10.1371/journal.pone.0202278 |
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