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Novel insight into an energy transduction mechanism of the bacterial flagellar type III protein export

Type III secretion system (T3SS) is a protein translocator complex family including pathogenic injectisome or bacterial flagellum. The inejectisomal T3SS serves to deliver virulence proteins into host cell and the flagellar T3SS constructs the flagellar axial structure. Although earlier studies have...

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Detalles Bibliográficos
Autores principales: Terashima, Hiroyuki, Imada, Katsumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society of Japan (BSJ) 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6145943/
https://www.ncbi.nlm.nih.gov/pubmed/30250776
http://dx.doi.org/10.2142/biophysico.15.0_173
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author Terashima, Hiroyuki
Imada, Katsumi
author_facet Terashima, Hiroyuki
Imada, Katsumi
author_sort Terashima, Hiroyuki
collection PubMed
description Type III secretion system (T3SS) is a protein translocator complex family including pathogenic injectisome or bacterial flagellum. The inejectisomal T3SS serves to deliver virulence proteins into host cell and the flagellar T3SS constructs the flagellar axial structure. Although earlier studies have provided many findings on the molecular mechanism of the Type III protein export, they were not sufficient to reveal energy transduction mechanism due to difficulties in controlling measurement conditions in vivo. Recently, we developed an in vitro flagellar Type III protein transport assay system using inverted membrane vesicles (IMVs), and analyzed protein export by using the in vitro method. We reproduced protein export of the flagellar T3SS, hook assembly and substrate specificity switch in IMV to a similar extent to what is seen in living cell. Furthermore, we demonstrated that ATP-hydrolysis energy can drive protein transport even in the absence of proton-motive force (PMF). In this mini-review, we will summarize our new in vitro Type III transport assay method and our findings on the molecular mechanism of Type III protein export.
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spelling pubmed-61459432018-09-24 Novel insight into an energy transduction mechanism of the bacterial flagellar type III protein export Terashima, Hiroyuki Imada, Katsumi Biophys Physicobiol Review Article Type III secretion system (T3SS) is a protein translocator complex family including pathogenic injectisome or bacterial flagellum. The inejectisomal T3SS serves to deliver virulence proteins into host cell and the flagellar T3SS constructs the flagellar axial structure. Although earlier studies have provided many findings on the molecular mechanism of the Type III protein export, they were not sufficient to reveal energy transduction mechanism due to difficulties in controlling measurement conditions in vivo. Recently, we developed an in vitro flagellar Type III protein transport assay system using inverted membrane vesicles (IMVs), and analyzed protein export by using the in vitro method. We reproduced protein export of the flagellar T3SS, hook assembly and substrate specificity switch in IMV to a similar extent to what is seen in living cell. Furthermore, we demonstrated that ATP-hydrolysis energy can drive protein transport even in the absence of proton-motive force (PMF). In this mini-review, we will summarize our new in vitro Type III transport assay method and our findings on the molecular mechanism of Type III protein export. The Biophysical Society of Japan (BSJ) 2018-08-22 /pmc/articles/PMC6145943/ /pubmed/30250776 http://dx.doi.org/10.2142/biophysico.15.0_173 Text en 2018 © The Biophysical Society of Japan This article is licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. To view a copy of this license, visit https://creativecommons.org/licenses/by-nc-sa/4.0/.
spellingShingle Review Article
Terashima, Hiroyuki
Imada, Katsumi
Novel insight into an energy transduction mechanism of the bacterial flagellar type III protein export
title Novel insight into an energy transduction mechanism of the bacterial flagellar type III protein export
title_full Novel insight into an energy transduction mechanism of the bacterial flagellar type III protein export
title_fullStr Novel insight into an energy transduction mechanism of the bacterial flagellar type III protein export
title_full_unstemmed Novel insight into an energy transduction mechanism of the bacterial flagellar type III protein export
title_short Novel insight into an energy transduction mechanism of the bacterial flagellar type III protein export
title_sort novel insight into an energy transduction mechanism of the bacterial flagellar type iii protein export
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6145943/
https://www.ncbi.nlm.nih.gov/pubmed/30250776
http://dx.doi.org/10.2142/biophysico.15.0_173
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