CCL3 Promotes Germinal Center B Cells Sampling by Follicular Regulatory T Cells in Murine Lymph Nodes

Previous studies and our findings suggest upregulated expression of proinflammatory chemokines CCL3/4 in germinal center (GC) centrocytes. However, the role of CCL3/4 for centrocyte interactions with follicular T cells and regulation of humoral immunity is poorly understood. We found that CCL3 promo...

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Autores principales: Benet, Zachary L., Marthi, Matangi, Ke, Fang, Wu, Rita, Turner, Jackson S., Gabayre, Jahan B., Ivanitskiy, Michael I., Sethi, Sahil S., Grigorova, Irina L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6146081/
https://www.ncbi.nlm.nih.gov/pubmed/30271404
http://dx.doi.org/10.3389/fimmu.2018.02044
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author Benet, Zachary L.
Marthi, Matangi
Ke, Fang
Wu, Rita
Turner, Jackson S.
Gabayre, Jahan B.
Ivanitskiy, Michael I.
Sethi, Sahil S.
Grigorova, Irina L.
author_facet Benet, Zachary L.
Marthi, Matangi
Ke, Fang
Wu, Rita
Turner, Jackson S.
Gabayre, Jahan B.
Ivanitskiy, Michael I.
Sethi, Sahil S.
Grigorova, Irina L.
author_sort Benet, Zachary L.
collection PubMed
description Previous studies and our findings suggest upregulated expression of proinflammatory chemokines CCL3/4 in germinal center (GC) centrocytes. However, the role of CCL3/4 for centrocyte interactions with follicular T cells and regulation of humoral immunity is poorly understood. We found that CCL3 promotes chemotaxis of Tfr cells ex vivo. Two-photon imaging revealed that B cells-intrinsic production of CCL3 promotes their probing by follicular regulatory T cells (Tfr) within GCs of murine lymph nodes. Overall this study suggests that CCL3 facilitates direct interactions of foreign antigen-specific GC B cells and their negative regulation with Tfr cells in vivo.
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spelling pubmed-61460812018-09-28 CCL3 Promotes Germinal Center B Cells Sampling by Follicular Regulatory T Cells in Murine Lymph Nodes Benet, Zachary L. Marthi, Matangi Ke, Fang Wu, Rita Turner, Jackson S. Gabayre, Jahan B. Ivanitskiy, Michael I. Sethi, Sahil S. Grigorova, Irina L. Front Immunol Immunology Previous studies and our findings suggest upregulated expression of proinflammatory chemokines CCL3/4 in germinal center (GC) centrocytes. However, the role of CCL3/4 for centrocyte interactions with follicular T cells and regulation of humoral immunity is poorly understood. We found that CCL3 promotes chemotaxis of Tfr cells ex vivo. Two-photon imaging revealed that B cells-intrinsic production of CCL3 promotes their probing by follicular regulatory T cells (Tfr) within GCs of murine lymph nodes. Overall this study suggests that CCL3 facilitates direct interactions of foreign antigen-specific GC B cells and their negative regulation with Tfr cells in vivo. Frontiers Media S.A. 2018-09-13 /pmc/articles/PMC6146081/ /pubmed/30271404 http://dx.doi.org/10.3389/fimmu.2018.02044 Text en Copyright © 2018 Benet, Marthi, Ke, Wu, Turner, Gabayre, Ivanitskiy, Sethi and Grigorova. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Benet, Zachary L.
Marthi, Matangi
Ke, Fang
Wu, Rita
Turner, Jackson S.
Gabayre, Jahan B.
Ivanitskiy, Michael I.
Sethi, Sahil S.
Grigorova, Irina L.
CCL3 Promotes Germinal Center B Cells Sampling by Follicular Regulatory T Cells in Murine Lymph Nodes
title CCL3 Promotes Germinal Center B Cells Sampling by Follicular Regulatory T Cells in Murine Lymph Nodes
title_full CCL3 Promotes Germinal Center B Cells Sampling by Follicular Regulatory T Cells in Murine Lymph Nodes
title_fullStr CCL3 Promotes Germinal Center B Cells Sampling by Follicular Regulatory T Cells in Murine Lymph Nodes
title_full_unstemmed CCL3 Promotes Germinal Center B Cells Sampling by Follicular Regulatory T Cells in Murine Lymph Nodes
title_short CCL3 Promotes Germinal Center B Cells Sampling by Follicular Regulatory T Cells in Murine Lymph Nodes
title_sort ccl3 promotes germinal center b cells sampling by follicular regulatory t cells in murine lymph nodes
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6146081/
https://www.ncbi.nlm.nih.gov/pubmed/30271404
http://dx.doi.org/10.3389/fimmu.2018.02044
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