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First description of plasmid mediated quinolone resistance genes in salmonella isolates from Saudi hospitals

OBJECTIVES: To detect resistance genes to fluoroquinolones and β-lactams in Salmonella strains from a Saudi hospital. METHODS: From October 2015 to December 2016, a total of 149 Salmonella strains were collected from stool specimens from patients admitted to King Fahad Hospital of the University, Al...

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Autores principales: Aljindan, Reem Y., Hussein, Nasreldin E., Khoudair, Hala A., Shaikh, Alaa Y., Hassan, Hoda A., Alabdulqader, Nawar A., Shorman, Mahmoud A., Abdalhamid, Baha A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Saudi Medical Journal 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6146258/
https://www.ncbi.nlm.nih.gov/pubmed/29968893
http://dx.doi.org/10.15537/smj.2018.7.22532
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author Aljindan, Reem Y.
Hussein, Nasreldin E.
Khoudair, Hala A.
Shaikh, Alaa Y.
Hassan, Hoda A.
Alabdulqader, Nawar A.
Shorman, Mahmoud A.
Abdalhamid, Baha A.
author_facet Aljindan, Reem Y.
Hussein, Nasreldin E.
Khoudair, Hala A.
Shaikh, Alaa Y.
Hassan, Hoda A.
Alabdulqader, Nawar A.
Shorman, Mahmoud A.
Abdalhamid, Baha A.
author_sort Aljindan, Reem Y.
collection PubMed
description OBJECTIVES: To detect resistance genes to fluoroquinolones and β-lactams in Salmonella strains from a Saudi hospital. METHODS: From October 2015 to December 2016, a total of 149 Salmonella strains were collected from stool specimens from patients admitted to King Fahad Hospital of the University, AlKhobar, Saudi Arabia using CHROMagar Salmonella. The organism identification and antimicrobial susceptibility testing were performed using Vitek 2 system. Strain serogrouping was performed using Wellcolex color Salmonella kit. Fluoroquinolone resistance genes, extended-spectrum β-lactamases (ESBLs), and AmpC β-lactamase were determined using polymerase chain reaction (PCR). Enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) was used to determine clonal relatedness. RESULTS: The resistance rates to cefotaxime were 1.3% and ciprofloxacin 19.5%. Plasmid mediated quinolone resistance (PMQR) genes, qnrB and qnrS, were detected in 8 strains, qnrB (n=5) and qnrS (n=3), respectively. No ESBLs, AmpC, or mutations in the topoisomerases were detected. Salmonella isolates formed 7 clusters with similarity. CONCLUSIONS: This study reveals the emergence of fluoroquinolone resistant Salmonella in the region imposing public health concerns.
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spelling pubmed-61462582018-09-28 First description of plasmid mediated quinolone resistance genes in salmonella isolates from Saudi hospitals Aljindan, Reem Y. Hussein, Nasreldin E. Khoudair, Hala A. Shaikh, Alaa Y. Hassan, Hoda A. Alabdulqader, Nawar A. Shorman, Mahmoud A. Abdalhamid, Baha A. Saudi Med J Original Article OBJECTIVES: To detect resistance genes to fluoroquinolones and β-lactams in Salmonella strains from a Saudi hospital. METHODS: From October 2015 to December 2016, a total of 149 Salmonella strains were collected from stool specimens from patients admitted to King Fahad Hospital of the University, AlKhobar, Saudi Arabia using CHROMagar Salmonella. The organism identification and antimicrobial susceptibility testing were performed using Vitek 2 system. Strain serogrouping was performed using Wellcolex color Salmonella kit. Fluoroquinolone resistance genes, extended-spectrum β-lactamases (ESBLs), and AmpC β-lactamase were determined using polymerase chain reaction (PCR). Enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) was used to determine clonal relatedness. RESULTS: The resistance rates to cefotaxime were 1.3% and ciprofloxacin 19.5%. Plasmid mediated quinolone resistance (PMQR) genes, qnrB and qnrS, were detected in 8 strains, qnrB (n=5) and qnrS (n=3), respectively. No ESBLs, AmpC, or mutations in the topoisomerases were detected. Salmonella isolates formed 7 clusters with similarity. CONCLUSIONS: This study reveals the emergence of fluoroquinolone resistant Salmonella in the region imposing public health concerns. Saudi Medical Journal 2018-07 /pmc/articles/PMC6146258/ /pubmed/29968893 http://dx.doi.org/10.15537/smj.2018.7.22532 Text en Copyright: © Saudi Medical Journal http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Aljindan, Reem Y.
Hussein, Nasreldin E.
Khoudair, Hala A.
Shaikh, Alaa Y.
Hassan, Hoda A.
Alabdulqader, Nawar A.
Shorman, Mahmoud A.
Abdalhamid, Baha A.
First description of plasmid mediated quinolone resistance genes in salmonella isolates from Saudi hospitals
title First description of plasmid mediated quinolone resistance genes in salmonella isolates from Saudi hospitals
title_full First description of plasmid mediated quinolone resistance genes in salmonella isolates from Saudi hospitals
title_fullStr First description of plasmid mediated quinolone resistance genes in salmonella isolates from Saudi hospitals
title_full_unstemmed First description of plasmid mediated quinolone resistance genes in salmonella isolates from Saudi hospitals
title_short First description of plasmid mediated quinolone resistance genes in salmonella isolates from Saudi hospitals
title_sort first description of plasmid mediated quinolone resistance genes in salmonella isolates from saudi hospitals
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6146258/
https://www.ncbi.nlm.nih.gov/pubmed/29968893
http://dx.doi.org/10.15537/smj.2018.7.22532
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