Determination of Aldehyde Dehydrogenase (ALDH) Isozymes in Human Cancer Samples - Comparison of Kinetic and Immunochemical Assays

A fluorimetric assay of aldehyde dehydrogenase isozymes, based on naphthaldehyde oxidation, is compared with Western Blotting analysis on several clinical samples obtained from surgery. The comparison reveals qualitatively good correlation of ALDH1A1 isozyme detection with two methods and somewhat w...

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Autores principales: Wroczynski, Piotr, Wierzchowski, Jacek, Gołąb, Jakub, Błażejczyk, Magdalena, Borecka, Dorota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2002
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6146554/
http://dx.doi.org/10.3390/71200896
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author Wroczynski, Piotr
Wierzchowski, Jacek
Gołąb, Jakub
Błażejczyk, Magdalena
Borecka, Dorota
author_facet Wroczynski, Piotr
Wierzchowski, Jacek
Gołąb, Jakub
Błażejczyk, Magdalena
Borecka, Dorota
author_sort Wroczynski, Piotr
collection PubMed
description A fluorimetric assay of aldehyde dehydrogenase isozymes, based on naphthaldehyde oxidation, is compared with Western Blotting analysis on several clinical samples obtained from surgery. The comparison reveals qualitatively good correlation of ALDH1A1 isozyme detection with two methods and somewhat worse on ALDH3A1 assay.
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spelling pubmed-61465542018-11-19 Determination of Aldehyde Dehydrogenase (ALDH) Isozymes in Human Cancer Samples - Comparison of Kinetic and Immunochemical Assays Wroczynski, Piotr Wierzchowski, Jacek Gołąb, Jakub Błażejczyk, Magdalena Borecka, Dorota Molecules Article A fluorimetric assay of aldehyde dehydrogenase isozymes, based on naphthaldehyde oxidation, is compared with Western Blotting analysis on several clinical samples obtained from surgery. The comparison reveals qualitatively good correlation of ALDH1A1 isozyme detection with two methods and somewhat worse on ALDH3A1 assay. MDPI 2002-12-31 /pmc/articles/PMC6146554/ http://dx.doi.org/10.3390/71200896 Text en © 2002 by MDPI (http://www.mdpi.org). Reproduction is permitted for noncommercial purposes.
spellingShingle Article
Wroczynski, Piotr
Wierzchowski, Jacek
Gołąb, Jakub
Błażejczyk, Magdalena
Borecka, Dorota
Determination of Aldehyde Dehydrogenase (ALDH) Isozymes in Human Cancer Samples - Comparison of Kinetic and Immunochemical Assays
title Determination of Aldehyde Dehydrogenase (ALDH) Isozymes in Human Cancer Samples - Comparison of Kinetic and Immunochemical Assays
title_full Determination of Aldehyde Dehydrogenase (ALDH) Isozymes in Human Cancer Samples - Comparison of Kinetic and Immunochemical Assays
title_fullStr Determination of Aldehyde Dehydrogenase (ALDH) Isozymes in Human Cancer Samples - Comparison of Kinetic and Immunochemical Assays
title_full_unstemmed Determination of Aldehyde Dehydrogenase (ALDH) Isozymes in Human Cancer Samples - Comparison of Kinetic and Immunochemical Assays
title_short Determination of Aldehyde Dehydrogenase (ALDH) Isozymes in Human Cancer Samples - Comparison of Kinetic and Immunochemical Assays
title_sort determination of aldehyde dehydrogenase (aldh) isozymes in human cancer samples - comparison of kinetic and immunochemical assays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6146554/
http://dx.doi.org/10.3390/71200896
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