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Porphyrin Dyes for Nonlinear Optical Imaging of Live Cells
Second harmonic generation (SHG)-based probes are useful for nonlinear optical imaging of biological structures, such as the plasma membrane. Several amphiphilic porphyrin-based dyes with high SHG coefficients have been synthesized with different hydrophilic head groups, and their cellular targeting...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147020/ https://www.ncbi.nlm.nih.gov/pubmed/30240737 http://dx.doi.org/10.1016/j.isci.2018.05.015 |
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author | Khadria, Anjul Fleischhauer, Jan Boczarow, Igor Wilkinson, James D. Kohl, Michael M. Anderson, Harry L. |
author_facet | Khadria, Anjul Fleischhauer, Jan Boczarow, Igor Wilkinson, James D. Kohl, Michael M. Anderson, Harry L. |
author_sort | Khadria, Anjul |
collection | PubMed |
description | Second harmonic generation (SHG)-based probes are useful for nonlinear optical imaging of biological structures, such as the plasma membrane. Several amphiphilic porphyrin-based dyes with high SHG coefficients have been synthesized with different hydrophilic head groups, and their cellular targeting has been studied. The probes with cationic head groups localize better at the plasma membrane than the neutral probes with zwitterionic or non-charged ethylene glycol-based head groups. Porphyrin dyes with only dications as hydrophilic head groups localize inside HEK293T cells to give SHG, whereas tricationic dyes localize robustly at the plasma membrane of cells, including neurons, in vitro and ex vivo. The copper(II) complex of the tricationic dye with negligible fluorescence quantum yield works as an SHG-only dye. The free-base tricationic dye has been demonstrated for two-photon fluorescence and SHG-based multimodal imaging. This study demonstrates the importance of a balance between the hydrophobicity and hydrophilicity of amphiphilic dyes for effective plasma membrane localization. |
format | Online Article Text |
id | pubmed-6147020 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-61470202018-10-02 Porphyrin Dyes for Nonlinear Optical Imaging of Live Cells Khadria, Anjul Fleischhauer, Jan Boczarow, Igor Wilkinson, James D. Kohl, Michael M. Anderson, Harry L. iScience Article Second harmonic generation (SHG)-based probes are useful for nonlinear optical imaging of biological structures, such as the plasma membrane. Several amphiphilic porphyrin-based dyes with high SHG coefficients have been synthesized with different hydrophilic head groups, and their cellular targeting has been studied. The probes with cationic head groups localize better at the plasma membrane than the neutral probes with zwitterionic or non-charged ethylene glycol-based head groups. Porphyrin dyes with only dications as hydrophilic head groups localize inside HEK293T cells to give SHG, whereas tricationic dyes localize robustly at the plasma membrane of cells, including neurons, in vitro and ex vivo. The copper(II) complex of the tricationic dye with negligible fluorescence quantum yield works as an SHG-only dye. The free-base tricationic dye has been demonstrated for two-photon fluorescence and SHG-based multimodal imaging. This study demonstrates the importance of a balance between the hydrophobicity and hydrophilicity of amphiphilic dyes for effective plasma membrane localization. Elsevier 2018-05-26 /pmc/articles/PMC6147020/ /pubmed/30240737 http://dx.doi.org/10.1016/j.isci.2018.05.015 Text en © 2018 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Khadria, Anjul Fleischhauer, Jan Boczarow, Igor Wilkinson, James D. Kohl, Michael M. Anderson, Harry L. Porphyrin Dyes for Nonlinear Optical Imaging of Live Cells |
title | Porphyrin Dyes for Nonlinear Optical Imaging of Live Cells |
title_full | Porphyrin Dyes for Nonlinear Optical Imaging of Live Cells |
title_fullStr | Porphyrin Dyes for Nonlinear Optical Imaging of Live Cells |
title_full_unstemmed | Porphyrin Dyes for Nonlinear Optical Imaging of Live Cells |
title_short | Porphyrin Dyes for Nonlinear Optical Imaging of Live Cells |
title_sort | porphyrin dyes for nonlinear optical imaging of live cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147020/ https://www.ncbi.nlm.nih.gov/pubmed/30240737 http://dx.doi.org/10.1016/j.isci.2018.05.015 |
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