Cargando…

The Antiapoptotic Effect of Curcumin in the Fibroblast of the Cochlea in an Ototoxic Rat Model

INTRODUCTION: This study aimed to show the potency of curcumin as an antiapoptotic agent that decreases the apoptotic index in the cochlea lateral wall in ototoxic rat models. MATERIALS AND METHODS: A total of 24 Rattus norvegicus were divided into eight groups: Group 1 (control group), Group 2 (gen...

Descripción completa

Detalles Bibliográficos
Autores principales: Haryuna, Tengku-Siti-Hajar, Purba, Agustinus-Hamonangan-Winston, Farhat, Farhat, Alviandi, Widayat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147266/
https://www.ncbi.nlm.nih.gov/pubmed/30245978
_version_ 1783356533994684416
author Haryuna, Tengku-Siti-Hajar
Purba, Agustinus-Hamonangan-Winston
Farhat, Farhat
Alviandi, Widayat
author_facet Haryuna, Tengku-Siti-Hajar
Purba, Agustinus-Hamonangan-Winston
Farhat, Farhat
Alviandi, Widayat
author_sort Haryuna, Tengku-Siti-Hajar
collection PubMed
description INTRODUCTION: This study aimed to show the potency of curcumin as an antiapoptotic agent that decreases the apoptotic index in the cochlea lateral wall in ototoxic rat models. MATERIALS AND METHODS: A total of 24 Rattus norvegicus were divided into eight groups: Group 1 (control group), Group 2 (gentamicin (+)), Group 3 (gentamicin + curcumin 20 mg/day), Group 4 (gentamicin + curcumin 40 mg/day), Group 5 (gentamicin + curcumin 20 mg/day for 7 days), Group 6 (gentamicin + curcumin 40 mg/day for 7 days), Group 7 (curcumin 20 mg/day for 3 days + gentamicin), and Group 8 (curcumin 40 mg/day for 3 days + gentamicin). After the division, the rats were terminated in order to measure the apoptotic index using a terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) assay in the fibroblasts of the cochlea lateral walls. The data were analyzed using analysis of variance (ANOVA), and P<0.05 was used as the cut-off for statistical significance. RESULTS: Administration of gentamicin showed significant differences (P<0.05) in the apoptotic index. Groups undergoing curcumin treatment at a higher dose (200 mg/kg bw) and the prevention groups showed significant differences compared with groups not treated with curcumin CONCLUSION: This study concluded that the apoptotic index can be decreased by curcumin and has a preventive benefit toward ototoxic rat models. The administration of curcumin depended on the dose and duration.
format Online
Article
Text
id pubmed-6147266
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Mashhad University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-61472662018-09-21 The Antiapoptotic Effect of Curcumin in the Fibroblast of the Cochlea in an Ototoxic Rat Model Haryuna, Tengku-Siti-Hajar Purba, Agustinus-Hamonangan-Winston Farhat, Farhat Alviandi, Widayat Iran J Otorhinolaryngol Original Article INTRODUCTION: This study aimed to show the potency of curcumin as an antiapoptotic agent that decreases the apoptotic index in the cochlea lateral wall in ototoxic rat models. MATERIALS AND METHODS: A total of 24 Rattus norvegicus were divided into eight groups: Group 1 (control group), Group 2 (gentamicin (+)), Group 3 (gentamicin + curcumin 20 mg/day), Group 4 (gentamicin + curcumin 40 mg/day), Group 5 (gentamicin + curcumin 20 mg/day for 7 days), Group 6 (gentamicin + curcumin 40 mg/day for 7 days), Group 7 (curcumin 20 mg/day for 3 days + gentamicin), and Group 8 (curcumin 40 mg/day for 3 days + gentamicin). After the division, the rats were terminated in order to measure the apoptotic index using a terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) assay in the fibroblasts of the cochlea lateral walls. The data were analyzed using analysis of variance (ANOVA), and P<0.05 was used as the cut-off for statistical significance. RESULTS: Administration of gentamicin showed significant differences (P<0.05) in the apoptotic index. Groups undergoing curcumin treatment at a higher dose (200 mg/kg bw) and the prevention groups showed significant differences compared with groups not treated with curcumin CONCLUSION: This study concluded that the apoptotic index can be decreased by curcumin and has a preventive benefit toward ototoxic rat models. The administration of curcumin depended on the dose and duration. Mashhad University of Medical Sciences 2018-09 /pmc/articles/PMC6147266/ /pubmed/30245978 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Haryuna, Tengku-Siti-Hajar
Purba, Agustinus-Hamonangan-Winston
Farhat, Farhat
Alviandi, Widayat
The Antiapoptotic Effect of Curcumin in the Fibroblast of the Cochlea in an Ototoxic Rat Model
title The Antiapoptotic Effect of Curcumin in the Fibroblast of the Cochlea in an Ototoxic Rat Model
title_full The Antiapoptotic Effect of Curcumin in the Fibroblast of the Cochlea in an Ototoxic Rat Model
title_fullStr The Antiapoptotic Effect of Curcumin in the Fibroblast of the Cochlea in an Ototoxic Rat Model
title_full_unstemmed The Antiapoptotic Effect of Curcumin in the Fibroblast of the Cochlea in an Ototoxic Rat Model
title_short The Antiapoptotic Effect of Curcumin in the Fibroblast of the Cochlea in an Ototoxic Rat Model
title_sort antiapoptotic effect of curcumin in the fibroblast of the cochlea in an ototoxic rat model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147266/
https://www.ncbi.nlm.nih.gov/pubmed/30245978
work_keys_str_mv AT haryunatengkusitihajar theantiapoptoticeffectofcurcumininthefibroblastofthecochleainanototoxicratmodel
AT purbaagustinushamonanganwinston theantiapoptoticeffectofcurcumininthefibroblastofthecochleainanototoxicratmodel
AT farhatfarhat theantiapoptoticeffectofcurcumininthefibroblastofthecochleainanototoxicratmodel
AT alviandiwidayat theantiapoptoticeffectofcurcumininthefibroblastofthecochleainanototoxicratmodel
AT haryunatengkusitihajar antiapoptoticeffectofcurcumininthefibroblastofthecochleainanototoxicratmodel
AT purbaagustinushamonanganwinston antiapoptoticeffectofcurcumininthefibroblastofthecochleainanototoxicratmodel
AT farhatfarhat antiapoptoticeffectofcurcumininthefibroblastofthecochleainanototoxicratmodel
AT alviandiwidayat antiapoptoticeffectofcurcumininthefibroblastofthecochleainanototoxicratmodel