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Pulsed-field gel electrophoresis of enterotoxic Clostridium perfringens type A isolates recovered from humans and animals in Kolkata, India

Clostridium perfringens is one of the most important globally recognised gastroenteric pathogen in humans as well as animals. The present study was aimed to know the similarities/divergence among C. perfringens type A isolates of human and animal origin using the pulsed-field gel electrophoresis (PF...

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Autores principales: Yadav, Jay P., Das, Suresh C., Dhaka, Pankaj, Mukhopadhyay, Asish K., Chowdhury, Goutam, Naskar, Syamal, Malik, Satyaveer S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculty of Veterinary Medicine, Cairo University 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147381/
https://www.ncbi.nlm.nih.gov/pubmed/30255089
http://dx.doi.org/10.1016/j.ijvsm.2017.11.002
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author Yadav, Jay P.
Das, Suresh C.
Dhaka, Pankaj
Mukhopadhyay, Asish K.
Chowdhury, Goutam
Naskar, Syamal
Malik, Satyaveer S.
author_facet Yadav, Jay P.
Das, Suresh C.
Dhaka, Pankaj
Mukhopadhyay, Asish K.
Chowdhury, Goutam
Naskar, Syamal
Malik, Satyaveer S.
author_sort Yadav, Jay P.
collection PubMed
description Clostridium perfringens is one of the most important globally recognised gastroenteric pathogen in humans as well as animals. The present study was aimed to know the similarities/divergence among C. perfringens type A isolates of human and animal origin using the pulsed-field gel electrophoresis (PFGE) as a molecular tool. The enterotoxic isolates obtained by screening of human diarrhoeal cases (n = 130), diarrhoeal cases of pig (n = 52) and goat (n = 50), meat samples viz., pork (n = 59) and chevon (n = 57) were characterized by standard cultural and biochemical methods followed by PCR Assays. Accordingly, a total of 11 C. perfringens type A characterized isolates (16S rRNA(+), cpa(+), cpb(2)(+) and cpe(+)) recovered from human diarrhoeal cases (n = 3); diarrhoeal cases of pig (n = 2) and goat (n = 2); meat samples viz. pork (n = 2) and chevon (n = 2) were examined employing PFGE. The observed clustering pattern in PFGE analysis showed the relatedness between isolates from diarrhoeal goat and chevon (90–100%); diarrhoeal pig and pork (65–68%); moreover, isolates from human diarrhoeal cases were exhibiting lineage to cases from goat and pig diarrhoea as well pork and chevon by 62–68% relatedness. The outcome of the present study indicates the probable contamination of this pathogen to the human food chain through faeces from suspected food animals viz. goat and pig and their improperly cooked meat.
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spelling pubmed-61473812018-09-25 Pulsed-field gel electrophoresis of enterotoxic Clostridium perfringens type A isolates recovered from humans and animals in Kolkata, India Yadav, Jay P. Das, Suresh C. Dhaka, Pankaj Mukhopadhyay, Asish K. Chowdhury, Goutam Naskar, Syamal Malik, Satyaveer S. Int J Vet Sci Med Short Communication Clostridium perfringens is one of the most important globally recognised gastroenteric pathogen in humans as well as animals. The present study was aimed to know the similarities/divergence among C. perfringens type A isolates of human and animal origin using the pulsed-field gel electrophoresis (PFGE) as a molecular tool. The enterotoxic isolates obtained by screening of human diarrhoeal cases (n = 130), diarrhoeal cases of pig (n = 52) and goat (n = 50), meat samples viz., pork (n = 59) and chevon (n = 57) were characterized by standard cultural and biochemical methods followed by PCR Assays. Accordingly, a total of 11 C. perfringens type A characterized isolates (16S rRNA(+), cpa(+), cpb(2)(+) and cpe(+)) recovered from human diarrhoeal cases (n = 3); diarrhoeal cases of pig (n = 2) and goat (n = 2); meat samples viz. pork (n = 2) and chevon (n = 2) were examined employing PFGE. The observed clustering pattern in PFGE analysis showed the relatedness between isolates from diarrhoeal goat and chevon (90–100%); diarrhoeal pig and pork (65–68%); moreover, isolates from human diarrhoeal cases were exhibiting lineage to cases from goat and pig diarrhoea as well pork and chevon by 62–68% relatedness. The outcome of the present study indicates the probable contamination of this pathogen to the human food chain through faeces from suspected food animals viz. goat and pig and their improperly cooked meat. Faculty of Veterinary Medicine, Cairo University 2017-12-15 /pmc/articles/PMC6147381/ /pubmed/30255089 http://dx.doi.org/10.1016/j.ijvsm.2017.11.002 Text en © 2017 Faculty of Veterinary Medicine, Cairo University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Short Communication
Yadav, Jay P.
Das, Suresh C.
Dhaka, Pankaj
Mukhopadhyay, Asish K.
Chowdhury, Goutam
Naskar, Syamal
Malik, Satyaveer S.
Pulsed-field gel electrophoresis of enterotoxic Clostridium perfringens type A isolates recovered from humans and animals in Kolkata, India
title Pulsed-field gel electrophoresis of enterotoxic Clostridium perfringens type A isolates recovered from humans and animals in Kolkata, India
title_full Pulsed-field gel electrophoresis of enterotoxic Clostridium perfringens type A isolates recovered from humans and animals in Kolkata, India
title_fullStr Pulsed-field gel electrophoresis of enterotoxic Clostridium perfringens type A isolates recovered from humans and animals in Kolkata, India
title_full_unstemmed Pulsed-field gel electrophoresis of enterotoxic Clostridium perfringens type A isolates recovered from humans and animals in Kolkata, India
title_short Pulsed-field gel electrophoresis of enterotoxic Clostridium perfringens type A isolates recovered from humans and animals in Kolkata, India
title_sort pulsed-field gel electrophoresis of enterotoxic clostridium perfringens type a isolates recovered from humans and animals in kolkata, india
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147381/
https://www.ncbi.nlm.nih.gov/pubmed/30255089
http://dx.doi.org/10.1016/j.ijvsm.2017.11.002
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