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Transcriptional outcomes and kinetic patterning of gene expression in response to NF-κB activation
Transcription factor nuclear factor kappa B (NF-κB) regulates cellular responses to environmental cues. Many stimuli induce NF-κB transiently, making time-dependent transcriptional outputs a fundamental feature of NF-κB activation. Here we show that NF-κB target genes have distinct kinetic patterns...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147668/ https://www.ncbi.nlm.nih.gov/pubmed/30199532 http://dx.doi.org/10.1371/journal.pbio.2006347 |
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author | Zhao, Mingming Joy, Jaimy Zhou, Weiqiang De, Supriyo Wood, William H. Becker, Kevin G. Ji, Hongkai Sen, Ranjan |
author_facet | Zhao, Mingming Joy, Jaimy Zhou, Weiqiang De, Supriyo Wood, William H. Becker, Kevin G. Ji, Hongkai Sen, Ranjan |
author_sort | Zhao, Mingming |
collection | PubMed |
description | Transcription factor nuclear factor kappa B (NF-κB) regulates cellular responses to environmental cues. Many stimuli induce NF-κB transiently, making time-dependent transcriptional outputs a fundamental feature of NF-κB activation. Here we show that NF-κB target genes have distinct kinetic patterns in activated B lymphoma cells. By combining RELA binding, RNA polymerase II (Pol II) recruitment, and perturbation of NF-κB activation, we demonstrate that kinetic differences amongst early- and late-activated RELA target genes can be understood based on chromatin configuration prior to cell activation and RELA-dependent priming, respectively. We also identified genes that were repressed by RELA activation and others that responded to RELA-activated transcription factors. Cumulatively, our studies define an NF-κB-responsive inducible gene cascade in activated B cells. |
format | Online Article Text |
id | pubmed-6147668 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-61476682018-10-08 Transcriptional outcomes and kinetic patterning of gene expression in response to NF-κB activation Zhao, Mingming Joy, Jaimy Zhou, Weiqiang De, Supriyo Wood, William H. Becker, Kevin G. Ji, Hongkai Sen, Ranjan PLoS Biol Research Article Transcription factor nuclear factor kappa B (NF-κB) regulates cellular responses to environmental cues. Many stimuli induce NF-κB transiently, making time-dependent transcriptional outputs a fundamental feature of NF-κB activation. Here we show that NF-κB target genes have distinct kinetic patterns in activated B lymphoma cells. By combining RELA binding, RNA polymerase II (Pol II) recruitment, and perturbation of NF-κB activation, we demonstrate that kinetic differences amongst early- and late-activated RELA target genes can be understood based on chromatin configuration prior to cell activation and RELA-dependent priming, respectively. We also identified genes that were repressed by RELA activation and others that responded to RELA-activated transcription factors. Cumulatively, our studies define an NF-κB-responsive inducible gene cascade in activated B cells. Public Library of Science 2018-09-10 /pmc/articles/PMC6147668/ /pubmed/30199532 http://dx.doi.org/10.1371/journal.pbio.2006347 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Zhao, Mingming Joy, Jaimy Zhou, Weiqiang De, Supriyo Wood, William H. Becker, Kevin G. Ji, Hongkai Sen, Ranjan Transcriptional outcomes and kinetic patterning of gene expression in response to NF-κB activation |
title | Transcriptional outcomes and kinetic patterning of gene expression in response to NF-κB activation |
title_full | Transcriptional outcomes and kinetic patterning of gene expression in response to NF-κB activation |
title_fullStr | Transcriptional outcomes and kinetic patterning of gene expression in response to NF-κB activation |
title_full_unstemmed | Transcriptional outcomes and kinetic patterning of gene expression in response to NF-κB activation |
title_short | Transcriptional outcomes and kinetic patterning of gene expression in response to NF-κB activation |
title_sort | transcriptional outcomes and kinetic patterning of gene expression in response to nf-κb activation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147668/ https://www.ncbi.nlm.nih.gov/pubmed/30199532 http://dx.doi.org/10.1371/journal.pbio.2006347 |
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