Robust single-cell DNA methylome profiling with snmC-seq2

Single-cell DNA methylome profiling has enabled the study of epigenomic heterogeneity in complex tissues and during cellular reprogramming. However, broader applications of the method have been impeded by the modest quality of sequencing libraries. Here we report snmC-seq2, which provides improved r...

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Autores principales: Luo, Chongyuan, Rivkin, Angeline, Zhou, Jingtian, Sandoval, Justin P., Kurihara, Laurie, Lucero, Jacinta, Castanon, Rosa, Nery, Joseph R., Pinto-Duarte, António, Bui, Brian, Fitzpatrick, Conor, O’Connor, Carolyn, Ruga, Seth, Van Eden, Marc E., Davis, David A., Mash, Deborah C., Behrens, M. Margarita, Ecker, Joseph R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147798/
https://www.ncbi.nlm.nih.gov/pubmed/30237449
http://dx.doi.org/10.1038/s41467-018-06355-2
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author Luo, Chongyuan
Rivkin, Angeline
Zhou, Jingtian
Sandoval, Justin P.
Kurihara, Laurie
Lucero, Jacinta
Castanon, Rosa
Nery, Joseph R.
Pinto-Duarte, António
Bui, Brian
Fitzpatrick, Conor
O’Connor, Carolyn
Ruga, Seth
Van Eden, Marc E.
Davis, David A.
Mash, Deborah C.
Behrens, M. Margarita
Ecker, Joseph R.
author_facet Luo, Chongyuan
Rivkin, Angeline
Zhou, Jingtian
Sandoval, Justin P.
Kurihara, Laurie
Lucero, Jacinta
Castanon, Rosa
Nery, Joseph R.
Pinto-Duarte, António
Bui, Brian
Fitzpatrick, Conor
O’Connor, Carolyn
Ruga, Seth
Van Eden, Marc E.
Davis, David A.
Mash, Deborah C.
Behrens, M. Margarita
Ecker, Joseph R.
author_sort Luo, Chongyuan
collection PubMed
description Single-cell DNA methylome profiling has enabled the study of epigenomic heterogeneity in complex tissues and during cellular reprogramming. However, broader applications of the method have been impeded by the modest quality of sequencing libraries. Here we report snmC-seq2, which provides improved read mapping, reduced artifactual reads, enhanced throughput, as well as increased library complexity and coverage uniformity compared to snmC-seq. snmC-seq2 is an efficient strategy suited for large-scale single-cell epigenomic studies.
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spelling pubmed-61477982018-09-25 Robust single-cell DNA methylome profiling with snmC-seq2 Luo, Chongyuan Rivkin, Angeline Zhou, Jingtian Sandoval, Justin P. Kurihara, Laurie Lucero, Jacinta Castanon, Rosa Nery, Joseph R. Pinto-Duarte, António Bui, Brian Fitzpatrick, Conor O’Connor, Carolyn Ruga, Seth Van Eden, Marc E. Davis, David A. Mash, Deborah C. Behrens, M. Margarita Ecker, Joseph R. Nat Commun Article Single-cell DNA methylome profiling has enabled the study of epigenomic heterogeneity in complex tissues and during cellular reprogramming. However, broader applications of the method have been impeded by the modest quality of sequencing libraries. Here we report snmC-seq2, which provides improved read mapping, reduced artifactual reads, enhanced throughput, as well as increased library complexity and coverage uniformity compared to snmC-seq. snmC-seq2 is an efficient strategy suited for large-scale single-cell epigenomic studies. Nature Publishing Group UK 2018-09-20 /pmc/articles/PMC6147798/ /pubmed/30237449 http://dx.doi.org/10.1038/s41467-018-06355-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Luo, Chongyuan
Rivkin, Angeline
Zhou, Jingtian
Sandoval, Justin P.
Kurihara, Laurie
Lucero, Jacinta
Castanon, Rosa
Nery, Joseph R.
Pinto-Duarte, António
Bui, Brian
Fitzpatrick, Conor
O’Connor, Carolyn
Ruga, Seth
Van Eden, Marc E.
Davis, David A.
Mash, Deborah C.
Behrens, M. Margarita
Ecker, Joseph R.
Robust single-cell DNA methylome profiling with snmC-seq2
title Robust single-cell DNA methylome profiling with snmC-seq2
title_full Robust single-cell DNA methylome profiling with snmC-seq2
title_fullStr Robust single-cell DNA methylome profiling with snmC-seq2
title_full_unstemmed Robust single-cell DNA methylome profiling with snmC-seq2
title_short Robust single-cell DNA methylome profiling with snmC-seq2
title_sort robust single-cell dna methylome profiling with snmc-seq2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147798/
https://www.ncbi.nlm.nih.gov/pubmed/30237449
http://dx.doi.org/10.1038/s41467-018-06355-2
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