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Genotyping of rifampin-resistant Mycobacterium tuberculosis isolates from western Turkey

BACKGROUND: Although the rate of multiple drug resistance is high, there is no published data on the transmission rate of drug-resistant strains of Mycobacterium tuberculosis in the Aegean region of western Turkey that are based on molecular methods. METHODS: IS6110 and pTBN12 restriction fragment l...

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Autores principales: Cavusoglu, Cengiz, Durmaz, Riza, Bilgic, Altinay, Gunal, Selami
Formato: Online Artículo Texto
Lenguaje:English
Publicado: King Faisal Specialist Hospital and Research Centre 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147894/
https://www.ncbi.nlm.nih.gov/pubmed/15323270
http://dx.doi.org/10.5144/0256-4947.2004.102
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author Cavusoglu, Cengiz
Durmaz, Riza
Bilgic, Altinay
Gunal, Selami
author_facet Cavusoglu, Cengiz
Durmaz, Riza
Bilgic, Altinay
Gunal, Selami
author_sort Cavusoglu, Cengiz
collection PubMed
description BACKGROUND: Although the rate of multiple drug resistance is high, there is no published data on the transmission rate of drug-resistant strains of Mycobacterium tuberculosis in the Aegean region of western Turkey that are based on molecular methods. METHODS: IS6110 and pTBN12 restriction fragment length polymorphism (RFLP) methods were used for typing M. tuberculosis strains isolated from 26 sputum samples from 26 patients. RESULTS: Nineteen of the rifampin-resistant isolates (73.1%) contained 6 to 11 copies of IS6110. Eighteen different IS6110 DNA fingerprint patterns were observed in the 26 rifampin-resistant isolates. Twenty-three of the 26 rifampin-resistant isolates were also resistant to isoniazid. When evaluated together, both methods yielded 21 (80.9%) different banding patterns and the level of clustering was 34.6%. The average number per pattern was 1.23 (26/21). CONCLUSIONS: IS6110 fingerprinting suggests that the rifampin-resistant isolates obtained from the Aegean region had a relatively high clustering rate and were clonally related. These findings showed that the rifampin-resistant isolates are actively transmitted between patients. Urgent measures should be taken to prevent the spread of these resistant strains.
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spelling pubmed-61478942018-09-21 Genotyping of rifampin-resistant Mycobacterium tuberculosis isolates from western Turkey Cavusoglu, Cengiz Durmaz, Riza Bilgic, Altinay Gunal, Selami Ann Saudi Med Original Article BACKGROUND: Although the rate of multiple drug resistance is high, there is no published data on the transmission rate of drug-resistant strains of Mycobacterium tuberculosis in the Aegean region of western Turkey that are based on molecular methods. METHODS: IS6110 and pTBN12 restriction fragment length polymorphism (RFLP) methods were used for typing M. tuberculosis strains isolated from 26 sputum samples from 26 patients. RESULTS: Nineteen of the rifampin-resistant isolates (73.1%) contained 6 to 11 copies of IS6110. Eighteen different IS6110 DNA fingerprint patterns were observed in the 26 rifampin-resistant isolates. Twenty-three of the 26 rifampin-resistant isolates were also resistant to isoniazid. When evaluated together, both methods yielded 21 (80.9%) different banding patterns and the level of clustering was 34.6%. The average number per pattern was 1.23 (26/21). CONCLUSIONS: IS6110 fingerprinting suggests that the rifampin-resistant isolates obtained from the Aegean region had a relatively high clustering rate and were clonally related. These findings showed that the rifampin-resistant isolates are actively transmitted between patients. Urgent measures should be taken to prevent the spread of these resistant strains. King Faisal Specialist Hospital and Research Centre 2004 /pmc/articles/PMC6147894/ /pubmed/15323270 http://dx.doi.org/10.5144/0256-4947.2004.102 Text en Copyright © 2004, Annals of Saudi Medicine This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Cavusoglu, Cengiz
Durmaz, Riza
Bilgic, Altinay
Gunal, Selami
Genotyping of rifampin-resistant Mycobacterium tuberculosis isolates from western Turkey
title Genotyping of rifampin-resistant Mycobacterium tuberculosis isolates from western Turkey
title_full Genotyping of rifampin-resistant Mycobacterium tuberculosis isolates from western Turkey
title_fullStr Genotyping of rifampin-resistant Mycobacterium tuberculosis isolates from western Turkey
title_full_unstemmed Genotyping of rifampin-resistant Mycobacterium tuberculosis isolates from western Turkey
title_short Genotyping of rifampin-resistant Mycobacterium tuberculosis isolates from western Turkey
title_sort genotyping of rifampin-resistant mycobacterium tuberculosis isolates from western turkey
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147894/
https://www.ncbi.nlm.nih.gov/pubmed/15323270
http://dx.doi.org/10.5144/0256-4947.2004.102
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