Cargando…

Diet induced changes in the microbiota and cell composition of rabbit gut associated lymphoid tissue (GALT)

The gut associated lymphoid tissue (GALT) is the largest immune organ of the body. Although the gut transient and mucosa-associated microbiota have been largely studied, the microbiota that colonizes the GALT has received less attention. The gut microbiome plays an important role in competitive excl...

Descripción completa

Detalles Bibliográficos
Autores principales: Arrazuria, Rakel, Pérez, Valentín, Molina, Elena, Juste, Ramón A., Khafipour, Ehsan, Elguezabal, Natalia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6148544/
https://www.ncbi.nlm.nih.gov/pubmed/30237566
http://dx.doi.org/10.1038/s41598-018-32484-1
_version_ 1783356752986636288
author Arrazuria, Rakel
Pérez, Valentín
Molina, Elena
Juste, Ramón A.
Khafipour, Ehsan
Elguezabal, Natalia
author_facet Arrazuria, Rakel
Pérez, Valentín
Molina, Elena
Juste, Ramón A.
Khafipour, Ehsan
Elguezabal, Natalia
author_sort Arrazuria, Rakel
collection PubMed
description The gut associated lymphoid tissue (GALT) is the largest immune organ of the body. Although the gut transient and mucosa-associated microbiota have been largely studied, the microbiota that colonizes the GALT has received less attention. The gut microbiome plays an important role in competitive exclusion of pathogens and in development and maturation of immunity. Diet is a key factor affecting the microbiota composition in the digestive tract. To investigate the relation between diet, microbiota and GALT, microbial and cell composition of vermiform appendix (VA) and sacculus rotundus (SR) were studied in two groups of New Zealand white rabbits on different diets. Diet shifted the lymphoid tissue microbiota affecting the presence and/or absence of certain taxa and their abundances. Immunohistochemistry revealed that a higher fibre content diet resulted in M cell hyperplasia and an increase of recently recruited macrophages, whereas T-cell levels remained unaltered in animals on both high fibre and standard diets. These findings indicate that diet has an impact on the microbiota and cell composition of the GALT, which could act as an important microbial recognition site where interactions with beneficial bacteria can take place favouring microbiota replacement after digestive dysregulations.
format Online
Article
Text
id pubmed-6148544
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-61485442019-02-12 Diet induced changes in the microbiota and cell composition of rabbit gut associated lymphoid tissue (GALT) Arrazuria, Rakel Pérez, Valentín Molina, Elena Juste, Ramón A. Khafipour, Ehsan Elguezabal, Natalia Sci Rep Article The gut associated lymphoid tissue (GALT) is the largest immune organ of the body. Although the gut transient and mucosa-associated microbiota have been largely studied, the microbiota that colonizes the GALT has received less attention. The gut microbiome plays an important role in competitive exclusion of pathogens and in development and maturation of immunity. Diet is a key factor affecting the microbiota composition in the digestive tract. To investigate the relation between diet, microbiota and GALT, microbial and cell composition of vermiform appendix (VA) and sacculus rotundus (SR) were studied in two groups of New Zealand white rabbits on different diets. Diet shifted the lymphoid tissue microbiota affecting the presence and/or absence of certain taxa and their abundances. Immunohistochemistry revealed that a higher fibre content diet resulted in M cell hyperplasia and an increase of recently recruited macrophages, whereas T-cell levels remained unaltered in animals on both high fibre and standard diets. These findings indicate that diet has an impact on the microbiota and cell composition of the GALT, which could act as an important microbial recognition site where interactions with beneficial bacteria can take place favouring microbiota replacement after digestive dysregulations. Nature Publishing Group UK 2018-09-20 /pmc/articles/PMC6148544/ /pubmed/30237566 http://dx.doi.org/10.1038/s41598-018-32484-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Arrazuria, Rakel
Pérez, Valentín
Molina, Elena
Juste, Ramón A.
Khafipour, Ehsan
Elguezabal, Natalia
Diet induced changes in the microbiota and cell composition of rabbit gut associated lymphoid tissue (GALT)
title Diet induced changes in the microbiota and cell composition of rabbit gut associated lymphoid tissue (GALT)
title_full Diet induced changes in the microbiota and cell composition of rabbit gut associated lymphoid tissue (GALT)
title_fullStr Diet induced changes in the microbiota and cell composition of rabbit gut associated lymphoid tissue (GALT)
title_full_unstemmed Diet induced changes in the microbiota and cell composition of rabbit gut associated lymphoid tissue (GALT)
title_short Diet induced changes in the microbiota and cell composition of rabbit gut associated lymphoid tissue (GALT)
title_sort diet induced changes in the microbiota and cell composition of rabbit gut associated lymphoid tissue (galt)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6148544/
https://www.ncbi.nlm.nih.gov/pubmed/30237566
http://dx.doi.org/10.1038/s41598-018-32484-1
work_keys_str_mv AT arrazuriarakel dietinducedchangesinthemicrobiotaandcellcompositionofrabbitgutassociatedlymphoidtissuegalt
AT perezvalentin dietinducedchangesinthemicrobiotaandcellcompositionofrabbitgutassociatedlymphoidtissuegalt
AT molinaelena dietinducedchangesinthemicrobiotaandcellcompositionofrabbitgutassociatedlymphoidtissuegalt
AT justeramona dietinducedchangesinthemicrobiotaandcellcompositionofrabbitgutassociatedlymphoidtissuegalt
AT khafipourehsan dietinducedchangesinthemicrobiotaandcellcompositionofrabbitgutassociatedlymphoidtissuegalt
AT elguezabalnatalia dietinducedchangesinthemicrobiotaandcellcompositionofrabbitgutassociatedlymphoidtissuegalt