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Distribution and genetic diversity of Blastocystis subtypes in various mammal and bird species in northeastern China

BACKGROUND: Blastocystis is one of the most common intestinal parasites in humans and animals worldwide. At least 17 subtypes have been identified in mammals and birds. In China, although some studies have reported the occurrence of Blastocystis in humans and animals, our understanding of the role o...

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Autores principales: Wang, Jianguang, Gong, Baiyan, Liu, Xiaohua, Zhao, Wei, Bu, Tong, Zhang, Weizhe, Liu, Aiqin, Yang, Fengkun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6148767/
https://www.ncbi.nlm.nih.gov/pubmed/30236147
http://dx.doi.org/10.1186/s13071-018-3106-z
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author Wang, Jianguang
Gong, Baiyan
Liu, Xiaohua
Zhao, Wei
Bu, Tong
Zhang, Weizhe
Liu, Aiqin
Yang, Fengkun
author_facet Wang, Jianguang
Gong, Baiyan
Liu, Xiaohua
Zhao, Wei
Bu, Tong
Zhang, Weizhe
Liu, Aiqin
Yang, Fengkun
author_sort Wang, Jianguang
collection PubMed
description BACKGROUND: Blastocystis is one of the most common intestinal parasites in humans and animals worldwide. At least 17 subtypes have been identified in mammals and birds. In China, although some studies have reported the occurrence of Blastocystis in humans and animals, our understanding of the role of animals in the transmission of human blastocystosis is only superficial due to a paucity of available molecular data. The aim of the present study was to understand infection rates of Blastocystis and the distribution and genetic diversity of subtypes in various mammal and bird species in northeastern China, as well as to assess the zoonotic potential of Blastocystis isolates. METHODS: A total of 1265 fresh fecal specimens (1080 from ten mammal species and 185 from eight bird species) were collected in Heilongjiang, Liaoning and Jilin provinces of China. Each specimen was examined for the presence of Blastocystis by PCR amplification and sequence analysis of the partial SSU rRNA gene. RESULTS: Fifty-four specimens (4.3%) were positive for Blastocystis. Birds (7.0%) had a higher infection rate of Blastocystis than mammals (3.8%). Blastocystis was found in seven mammal species, reindeer (6.7%), sika deer (14.6%), racoon dogs (7.5%), Arctic foxes (1.9%), dogs (2.9%), rats (3.7%) and rabbits (3.3%), as well as three bird species, pigeons (2.1%), chickens (13.0%) and red crowned cranes (14.0%). Eight subtypes were identified including ST1 (n = 5), ST3 (n = 3), ST4 (n = 13), ST6 (n = 8), ST7 (n = 6), ST10 (n = 13), ST13 (n = 4) and ST14 (n = 2). 64.8% (35/54) of Blastocystis isolates belonged to potentially zoonotic subtypes. CONCLUSIONS: To our knowledge, this is the first report of Blastocystis in reindeer (ST10 and ST13), rabbits (ST4), racoon dogs (ST3) and Arctic foxes (ST1, ST4 and ST7). The findings of potentially zoonotic subtypes suggest that the animals infected with Blastocystis might pose a threat to human health. These data will improve our understanding of the host range and genetic diversity of Blastocystis, and also help develop efficient control strategies to intervene with and prevent the occurrence of human blastocystosis in the investigated areas.
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spelling pubmed-61487672018-09-24 Distribution and genetic diversity of Blastocystis subtypes in various mammal and bird species in northeastern China Wang, Jianguang Gong, Baiyan Liu, Xiaohua Zhao, Wei Bu, Tong Zhang, Weizhe Liu, Aiqin Yang, Fengkun Parasit Vectors Research BACKGROUND: Blastocystis is one of the most common intestinal parasites in humans and animals worldwide. At least 17 subtypes have been identified in mammals and birds. In China, although some studies have reported the occurrence of Blastocystis in humans and animals, our understanding of the role of animals in the transmission of human blastocystosis is only superficial due to a paucity of available molecular data. The aim of the present study was to understand infection rates of Blastocystis and the distribution and genetic diversity of subtypes in various mammal and bird species in northeastern China, as well as to assess the zoonotic potential of Blastocystis isolates. METHODS: A total of 1265 fresh fecal specimens (1080 from ten mammal species and 185 from eight bird species) were collected in Heilongjiang, Liaoning and Jilin provinces of China. Each specimen was examined for the presence of Blastocystis by PCR amplification and sequence analysis of the partial SSU rRNA gene. RESULTS: Fifty-four specimens (4.3%) were positive for Blastocystis. Birds (7.0%) had a higher infection rate of Blastocystis than mammals (3.8%). Blastocystis was found in seven mammal species, reindeer (6.7%), sika deer (14.6%), racoon dogs (7.5%), Arctic foxes (1.9%), dogs (2.9%), rats (3.7%) and rabbits (3.3%), as well as three bird species, pigeons (2.1%), chickens (13.0%) and red crowned cranes (14.0%). Eight subtypes were identified including ST1 (n = 5), ST3 (n = 3), ST4 (n = 13), ST6 (n = 8), ST7 (n = 6), ST10 (n = 13), ST13 (n = 4) and ST14 (n = 2). 64.8% (35/54) of Blastocystis isolates belonged to potentially zoonotic subtypes. CONCLUSIONS: To our knowledge, this is the first report of Blastocystis in reindeer (ST10 and ST13), rabbits (ST4), racoon dogs (ST3) and Arctic foxes (ST1, ST4 and ST7). The findings of potentially zoonotic subtypes suggest that the animals infected with Blastocystis might pose a threat to human health. These data will improve our understanding of the host range and genetic diversity of Blastocystis, and also help develop efficient control strategies to intervene with and prevent the occurrence of human blastocystosis in the investigated areas. BioMed Central 2018-09-20 /pmc/articles/PMC6148767/ /pubmed/30236147 http://dx.doi.org/10.1186/s13071-018-3106-z Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wang, Jianguang
Gong, Baiyan
Liu, Xiaohua
Zhao, Wei
Bu, Tong
Zhang, Weizhe
Liu, Aiqin
Yang, Fengkun
Distribution and genetic diversity of Blastocystis subtypes in various mammal and bird species in northeastern China
title Distribution and genetic diversity of Blastocystis subtypes in various mammal and bird species in northeastern China
title_full Distribution and genetic diversity of Blastocystis subtypes in various mammal and bird species in northeastern China
title_fullStr Distribution and genetic diversity of Blastocystis subtypes in various mammal and bird species in northeastern China
title_full_unstemmed Distribution and genetic diversity of Blastocystis subtypes in various mammal and bird species in northeastern China
title_short Distribution and genetic diversity of Blastocystis subtypes in various mammal and bird species in northeastern China
title_sort distribution and genetic diversity of blastocystis subtypes in various mammal and bird species in northeastern china
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6148767/
https://www.ncbi.nlm.nih.gov/pubmed/30236147
http://dx.doi.org/10.1186/s13071-018-3106-z
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