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The marsupial trypanosome Trypanosoma copemani is not an obligate intracellular parasite, although it adversely affects cell health
BACKGROUND: Trypanosoma cruzi invades and replicates inside mammalian cells, which can lead to chronic Chagas disease in humans. Trypanosoma copemani infects Australian marsupials and recent investigations indicate it may be able to invade mammalian cells in vitro, similar to T. cruzi. Here, T. cruz...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6148770/ https://www.ncbi.nlm.nih.gov/pubmed/30236162 http://dx.doi.org/10.1186/s13071-018-3092-1 |
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author | Cooper, Crystal Andrew Thompson, R. C. Rigby, Paul Buckley, Alysia Peacock, Christopher Clode, Peta L. |
author_facet | Cooper, Crystal Andrew Thompson, R. C. Rigby, Paul Buckley, Alysia Peacock, Christopher Clode, Peta L. |
author_sort | Cooper, Crystal |
collection | PubMed |
description | BACKGROUND: Trypanosoma cruzi invades and replicates inside mammalian cells, which can lead to chronic Chagas disease in humans. Trypanosoma copemani infects Australian marsupials and recent investigations indicate it may be able to invade mammalian cells in vitro, similar to T. cruzi. Here, T. cruzi 10R26 strain (TcIIa) and two strains of T. copemani [genotype 1 (G1) and genotype 2 (G2)] were incubated with marsupial cells in vitro. Live-cell time-lapse and fluorescent microscopy, combined with high-resolution microscopy (transmission and scanning electron microscopy) were used to investigate surface interactions between parasites and mammalian cells. RESULTS: The number of parasites invading cells was significantly higher in T. cruzi compared to either genotype of T. copemani, between which there was no significant difference. While capable of cellular invasion, T. copemani did not multiply in host cells in vitro as there was no increase in intracellular amastigotes over time and no release of new trypomastigotes from host cells, as observed in T. cruzi. Exposure of host cells to G2 trypomastigotes resulted in increased host cell membrane permeability within 24 h of infection, and host cell death/blebbing was also observed. G2 parasites also became embedded in the host cell membrane. CONCLUSIONS: Trypanosoma copemani is unlikely to have an obligate intracellular life-cycle like T. cruzi. However, T. copemani adversely affects cell health in vitro and should be investigated in vivo in infected host tissues to better understand this host-parasite relationship. Future research should focus on increasing understanding of the T. copemani life history and the genetic, physiological and ecological differences between different genotypes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-018-3092-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6148770 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-61487702018-09-24 The marsupial trypanosome Trypanosoma copemani is not an obligate intracellular parasite, although it adversely affects cell health Cooper, Crystal Andrew Thompson, R. C. Rigby, Paul Buckley, Alysia Peacock, Christopher Clode, Peta L. Parasit Vectors Research BACKGROUND: Trypanosoma cruzi invades and replicates inside mammalian cells, which can lead to chronic Chagas disease in humans. Trypanosoma copemani infects Australian marsupials and recent investigations indicate it may be able to invade mammalian cells in vitro, similar to T. cruzi. Here, T. cruzi 10R26 strain (TcIIa) and two strains of T. copemani [genotype 1 (G1) and genotype 2 (G2)] were incubated with marsupial cells in vitro. Live-cell time-lapse and fluorescent microscopy, combined with high-resolution microscopy (transmission and scanning electron microscopy) were used to investigate surface interactions between parasites and mammalian cells. RESULTS: The number of parasites invading cells was significantly higher in T. cruzi compared to either genotype of T. copemani, between which there was no significant difference. While capable of cellular invasion, T. copemani did not multiply in host cells in vitro as there was no increase in intracellular amastigotes over time and no release of new trypomastigotes from host cells, as observed in T. cruzi. Exposure of host cells to G2 trypomastigotes resulted in increased host cell membrane permeability within 24 h of infection, and host cell death/blebbing was also observed. G2 parasites also became embedded in the host cell membrane. CONCLUSIONS: Trypanosoma copemani is unlikely to have an obligate intracellular life-cycle like T. cruzi. However, T. copemani adversely affects cell health in vitro and should be investigated in vivo in infected host tissues to better understand this host-parasite relationship. Future research should focus on increasing understanding of the T. copemani life history and the genetic, physiological and ecological differences between different genotypes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-018-3092-1) contains supplementary material, which is available to authorized users. BioMed Central 2018-09-20 /pmc/articles/PMC6148770/ /pubmed/30236162 http://dx.doi.org/10.1186/s13071-018-3092-1 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Cooper, Crystal Andrew Thompson, R. C. Rigby, Paul Buckley, Alysia Peacock, Christopher Clode, Peta L. The marsupial trypanosome Trypanosoma copemani is not an obligate intracellular parasite, although it adversely affects cell health |
title | The marsupial trypanosome Trypanosoma copemani is not an obligate intracellular parasite, although it adversely affects cell health |
title_full | The marsupial trypanosome Trypanosoma copemani is not an obligate intracellular parasite, although it adversely affects cell health |
title_fullStr | The marsupial trypanosome Trypanosoma copemani is not an obligate intracellular parasite, although it adversely affects cell health |
title_full_unstemmed | The marsupial trypanosome Trypanosoma copemani is not an obligate intracellular parasite, although it adversely affects cell health |
title_short | The marsupial trypanosome Trypanosoma copemani is not an obligate intracellular parasite, although it adversely affects cell health |
title_sort | marsupial trypanosome trypanosoma copemani is not an obligate intracellular parasite, although it adversely affects cell health |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6148770/ https://www.ncbi.nlm.nih.gov/pubmed/30236162 http://dx.doi.org/10.1186/s13071-018-3092-1 |
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