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A Novel Strategy for Quantitative Analysis of Major Ginsenosides in Panacis Japonici Rhizoma with a Standardized Reference Fraction

Panacis Japonici Rhizoma (Zhu-Jie-Shen in Chinese), the root of P. japonicus C.A. Mey., is commonly used in traditional Chinese Medicine. Saponins are the major bioactive compounds in this herb. The similarity of polarity and structure of the natural products in herb caused the difficulty of purific...

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Autores principales: Meng, Fan-Cheng, Wu, Qiu-Shuang, Wang, Ruibing, Li, Shao-Ping, Lin, Li-Gen, Chen, Ping, Zhang, Qing-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6149681/
https://www.ncbi.nlm.nih.gov/pubmed/29186881
http://dx.doi.org/10.3390/molecules22122067
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author Meng, Fan-Cheng
Wu, Qiu-Shuang
Wang, Ruibing
Li, Shao-Ping
Lin, Li-Gen
Chen, Ping
Zhang, Qing-Wen
author_facet Meng, Fan-Cheng
Wu, Qiu-Shuang
Wang, Ruibing
Li, Shao-Ping
Lin, Li-Gen
Chen, Ping
Zhang, Qing-Wen
author_sort Meng, Fan-Cheng
collection PubMed
description Panacis Japonici Rhizoma (Zhu-Jie-Shen in Chinese), the root of P. japonicus C.A. Mey., is commonly used in traditional Chinese Medicine. Saponins are the major bioactive compounds in this herb. The similarity of polarity and structure of the natural products in herb caused the difficulty of purification and resulted in the shortage and high cost of the reference compounds, which has greatly hindered efforts toward quantification in quality control. A novel strategy using a standardized reference fraction for qualification of the major saponins in Panacis Japonici Rhizoma was proposed to easily and effectively control the quality of PJR. The strategy is feasible and reliable, and the methodology of the developed approach is also validated. The standardized reference fraction was used for quantification, which might solve the shortage of the pure reference compounds in the quality control of herbal medicines.
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spelling pubmed-61496812018-11-13 A Novel Strategy for Quantitative Analysis of Major Ginsenosides in Panacis Japonici Rhizoma with a Standardized Reference Fraction Meng, Fan-Cheng Wu, Qiu-Shuang Wang, Ruibing Li, Shao-Ping Lin, Li-Gen Chen, Ping Zhang, Qing-Wen Molecules Article Panacis Japonici Rhizoma (Zhu-Jie-Shen in Chinese), the root of P. japonicus C.A. Mey., is commonly used in traditional Chinese Medicine. Saponins are the major bioactive compounds in this herb. The similarity of polarity and structure of the natural products in herb caused the difficulty of purification and resulted in the shortage and high cost of the reference compounds, which has greatly hindered efforts toward quantification in quality control. A novel strategy using a standardized reference fraction for qualification of the major saponins in Panacis Japonici Rhizoma was proposed to easily and effectively control the quality of PJR. The strategy is feasible and reliable, and the methodology of the developed approach is also validated. The standardized reference fraction was used for quantification, which might solve the shortage of the pure reference compounds in the quality control of herbal medicines. MDPI 2017-11-27 /pmc/articles/PMC6149681/ /pubmed/29186881 http://dx.doi.org/10.3390/molecules22122067 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Meng, Fan-Cheng
Wu, Qiu-Shuang
Wang, Ruibing
Li, Shao-Ping
Lin, Li-Gen
Chen, Ping
Zhang, Qing-Wen
A Novel Strategy for Quantitative Analysis of Major Ginsenosides in Panacis Japonici Rhizoma with a Standardized Reference Fraction
title A Novel Strategy for Quantitative Analysis of Major Ginsenosides in Panacis Japonici Rhizoma with a Standardized Reference Fraction
title_full A Novel Strategy for Quantitative Analysis of Major Ginsenosides in Panacis Japonici Rhizoma with a Standardized Reference Fraction
title_fullStr A Novel Strategy for Quantitative Analysis of Major Ginsenosides in Panacis Japonici Rhizoma with a Standardized Reference Fraction
title_full_unstemmed A Novel Strategy for Quantitative Analysis of Major Ginsenosides in Panacis Japonici Rhizoma with a Standardized Reference Fraction
title_short A Novel Strategy for Quantitative Analysis of Major Ginsenosides in Panacis Japonici Rhizoma with a Standardized Reference Fraction
title_sort novel strategy for quantitative analysis of major ginsenosides in panacis japonici rhizoma with a standardized reference fraction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6149681/
https://www.ncbi.nlm.nih.gov/pubmed/29186881
http://dx.doi.org/10.3390/molecules22122067
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