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Preparation of β-CD-Ellagic Acid Microspheres and Their Effects on HepG2 Cell Proliferation
Objective: In this study, β-cyclodextrin (β-CD) was chosen as the coating for ellagic acid to prepare ellagic acid microspheres, and the effect of microspheres on the growth of HepG2 cells was observed. Methods: Scanning electron microscopy, infrared spectroscopy, and release rate analysis were used...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6149914/ https://www.ncbi.nlm.nih.gov/pubmed/29292740 http://dx.doi.org/10.3390/molecules22122175 |
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author | Wang, Hongkai Zhang, Yingxia Tian, Zhongjing Ma, Jing Kang, Meiling Ding, Chengshi Ming, Dongfeng |
author_facet | Wang, Hongkai Zhang, Yingxia Tian, Zhongjing Ma, Jing Kang, Meiling Ding, Chengshi Ming, Dongfeng |
author_sort | Wang, Hongkai |
collection | PubMed |
description | Objective: In this study, β-cyclodextrin (β-CD) was chosen as the coating for ellagic acid to prepare ellagic acid microspheres, and the effect of microspheres on the growth of HepG2 cells was observed. Methods: Scanning electron microscopy, infrared spectroscopy, and release rate analysis were used to identify the formation of ellagic acid microspheres. Methyl thiazolyl tetrazolium (MTT) assay was used to detect the effect of different concentrations of ellagic acid microspheres on tumor cell proliferation at 6, 12, 24 and 36 h, and cell morphology and quantity were observed using hematoxylin-eosin (HE) staining. Single-cell gel electrophoresis was used to observe the effect of ellagic acid microspheres on the DNA damage of HepG2 cells, and the Olive tail moment and the mRNA expression of tumor suppressor protein gene p53 was measured. Results: β-CD could be used as wrapping material of ellagic acid to prepare ellagic acid microspheres. HepG2 cell proliferation could be inhibited by 0.1, 0.3 and 0.5 g/L of ellagic acid microspheres in a dose- and time-dependent manner, and the mechanism of proliferation inhibition was related to DNA damage and cell apoptosis. Conclusion: Preparing ellagic acid microspheres with β-CD is feasible, and ellagic acid microspheres have potential therapeutic value (anticancer). |
format | Online Article Text |
id | pubmed-6149914 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61499142018-11-13 Preparation of β-CD-Ellagic Acid Microspheres and Their Effects on HepG2 Cell Proliferation Wang, Hongkai Zhang, Yingxia Tian, Zhongjing Ma, Jing Kang, Meiling Ding, Chengshi Ming, Dongfeng Molecules Article Objective: In this study, β-cyclodextrin (β-CD) was chosen as the coating for ellagic acid to prepare ellagic acid microspheres, and the effect of microspheres on the growth of HepG2 cells was observed. Methods: Scanning electron microscopy, infrared spectroscopy, and release rate analysis were used to identify the formation of ellagic acid microspheres. Methyl thiazolyl tetrazolium (MTT) assay was used to detect the effect of different concentrations of ellagic acid microspheres on tumor cell proliferation at 6, 12, 24 and 36 h, and cell morphology and quantity were observed using hematoxylin-eosin (HE) staining. Single-cell gel electrophoresis was used to observe the effect of ellagic acid microspheres on the DNA damage of HepG2 cells, and the Olive tail moment and the mRNA expression of tumor suppressor protein gene p53 was measured. Results: β-CD could be used as wrapping material of ellagic acid to prepare ellagic acid microspheres. HepG2 cell proliferation could be inhibited by 0.1, 0.3 and 0.5 g/L of ellagic acid microspheres in a dose- and time-dependent manner, and the mechanism of proliferation inhibition was related to DNA damage and cell apoptosis. Conclusion: Preparing ellagic acid microspheres with β-CD is feasible, and ellagic acid microspheres have potential therapeutic value (anticancer). MDPI 2017-12-08 /pmc/articles/PMC6149914/ /pubmed/29292740 http://dx.doi.org/10.3390/molecules22122175 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Hongkai Zhang, Yingxia Tian, Zhongjing Ma, Jing Kang, Meiling Ding, Chengshi Ming, Dongfeng Preparation of β-CD-Ellagic Acid Microspheres and Their Effects on HepG2 Cell Proliferation |
title | Preparation of β-CD-Ellagic Acid Microspheres and Their Effects on HepG2 Cell Proliferation |
title_full | Preparation of β-CD-Ellagic Acid Microspheres and Their Effects on HepG2 Cell Proliferation |
title_fullStr | Preparation of β-CD-Ellagic Acid Microspheres and Their Effects on HepG2 Cell Proliferation |
title_full_unstemmed | Preparation of β-CD-Ellagic Acid Microspheres and Their Effects on HepG2 Cell Proliferation |
title_short | Preparation of β-CD-Ellagic Acid Microspheres and Their Effects on HepG2 Cell Proliferation |
title_sort | preparation of β-cd-ellagic acid microspheres and their effects on hepg2 cell proliferation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6149914/ https://www.ncbi.nlm.nih.gov/pubmed/29292740 http://dx.doi.org/10.3390/molecules22122175 |
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