Cargando…
Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro
The study is aimed at investigating the immunoenhancement activity of polysaccharides from Platycodon grandiflorum polysaccharides (PGPSs) in vitro. In this study, some research on lymphocyte proliferation, cell cycle, and the levels of CD4(+) and CD8(+) T cells were performed. Four different concen...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6150289/ https://www.ncbi.nlm.nih.gov/pubmed/29112148 http://dx.doi.org/10.3390/molecules22111918 |
_version_ | 1783356959641042944 |
---|---|
author | Zhao, Xiaona Wang, Yuge Yan, Peng Cheng, Guodong Wang, Cheng Geng, Na Wang, Xuepeng Liu, Jianzhu |
author_facet | Zhao, Xiaona Wang, Yuge Yan, Peng Cheng, Guodong Wang, Cheng Geng, Na Wang, Xuepeng Liu, Jianzhu |
author_sort | Zhao, Xiaona |
collection | PubMed |
description | The study is aimed at investigating the immunoenhancement activity of polysaccharides from Platycodon grandiflorum polysaccharides (PGPSs) in vitro. In this study, some research on lymphocyte proliferation, cell cycle, and the levels of CD4(+) and CD8(+) T cells were performed. Four different concentrations of PGPSs (PGPS(tc), PGPS(60c), PGPS(80c), and PGPS(tp)) were harvested and added to peripheral blood T lymphocytes. We observed significant increases in T lymphocyte proliferation at PGPS(tc) groups individually or synergistically with phytohemagglutinin (PHA) at most concentrations, and their lymphocyte proliferation rates were the highest. The active sites of PGPS(tc) and PGPS(60c) were subsequently chosen. Then, we utilized flow cytometry to determine lymphocyte cell cycle distribution and levels of CD4(+) and CD8(+) T cells. At most time points, PGPS(tc) could facilitate lymphocyte cell cycle progression from the G0/G1 phase to the S and G2/M phases and, simultaneously, increase the levels of CD4(+) and CD8(+) T cells. These results indicate that PGPS(tc) enhances the immune functions, suggesting that PGPS(tc) could be a potential immunopotentiator for further in vivo and clinical trial experiments. |
format | Online Article Text |
id | pubmed-6150289 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61502892018-11-13 Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro Zhao, Xiaona Wang, Yuge Yan, Peng Cheng, Guodong Wang, Cheng Geng, Na Wang, Xuepeng Liu, Jianzhu Molecules Article The study is aimed at investigating the immunoenhancement activity of polysaccharides from Platycodon grandiflorum polysaccharides (PGPSs) in vitro. In this study, some research on lymphocyte proliferation, cell cycle, and the levels of CD4(+) and CD8(+) T cells were performed. Four different concentrations of PGPSs (PGPS(tc), PGPS(60c), PGPS(80c), and PGPS(tp)) were harvested and added to peripheral blood T lymphocytes. We observed significant increases in T lymphocyte proliferation at PGPS(tc) groups individually or synergistically with phytohemagglutinin (PHA) at most concentrations, and their lymphocyte proliferation rates were the highest. The active sites of PGPS(tc) and PGPS(60c) were subsequently chosen. Then, we utilized flow cytometry to determine lymphocyte cell cycle distribution and levels of CD4(+) and CD8(+) T cells. At most time points, PGPS(tc) could facilitate lymphocyte cell cycle progression from the G0/G1 phase to the S and G2/M phases and, simultaneously, increase the levels of CD4(+) and CD8(+) T cells. These results indicate that PGPS(tc) enhances the immune functions, suggesting that PGPS(tc) could be a potential immunopotentiator for further in vivo and clinical trial experiments. MDPI 2017-11-07 /pmc/articles/PMC6150289/ /pubmed/29112148 http://dx.doi.org/10.3390/molecules22111918 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhao, Xiaona Wang, Yuge Yan, Peng Cheng, Guodong Wang, Cheng Geng, Na Wang, Xuepeng Liu, Jianzhu Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro |
title | Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro |
title_full | Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro |
title_fullStr | Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro |
title_full_unstemmed | Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro |
title_short | Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro |
title_sort | effects of polysaccharides from platycodon grandiflorum on immunity-enhancing activity in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6150289/ https://www.ncbi.nlm.nih.gov/pubmed/29112148 http://dx.doi.org/10.3390/molecules22111918 |
work_keys_str_mv | AT zhaoxiaona effectsofpolysaccharidesfromplatycodongrandiflorumonimmunityenhancingactivityinvitro AT wangyuge effectsofpolysaccharidesfromplatycodongrandiflorumonimmunityenhancingactivityinvitro AT yanpeng effectsofpolysaccharidesfromplatycodongrandiflorumonimmunityenhancingactivityinvitro AT chengguodong effectsofpolysaccharidesfromplatycodongrandiflorumonimmunityenhancingactivityinvitro AT wangcheng effectsofpolysaccharidesfromplatycodongrandiflorumonimmunityenhancingactivityinvitro AT gengna effectsofpolysaccharidesfromplatycodongrandiflorumonimmunityenhancingactivityinvitro AT wangxuepeng effectsofpolysaccharidesfromplatycodongrandiflorumonimmunityenhancingactivityinvitro AT liujianzhu effectsofpolysaccharidesfromplatycodongrandiflorumonimmunityenhancingactivityinvitro |