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Anti-Inflammatory Potential of 1-Nitro-2-Phenylethylene

Inflammation is a reaction of the host to infectious or sterile stimuli and has the physiological purpose of restoring tissue homeostasis. However, uncontrolled or unresolved inflammation can lead to tissue damage, giving rise to a plethora of chronic inflammatory diseases, including metabolic syndr...

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Autores principales: Sugimoto, Michelle A., de Jesus Amazonas da Silva, Márcia, Froede Brito, Larissa, dos Santos Borges, Rosivaldo, Amaral, Flávio Almeida, de Araujo Boleti, Ana Paula, Ordoñez, Maritza Echevarria, Carlos Tavares, Jose, Pires Sousa, Lirlandia, Lima, Emerson Silva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6150367/
https://www.ncbi.nlm.nih.gov/pubmed/29140265
http://dx.doi.org/10.3390/molecules22111977
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author Sugimoto, Michelle A.
de Jesus Amazonas da Silva, Márcia
Froede Brito, Larissa
dos Santos Borges, Rosivaldo
Amaral, Flávio Almeida
de Araujo Boleti, Ana Paula
Ordoñez, Maritza Echevarria
Carlos Tavares, Jose
Pires Sousa, Lirlandia
Lima, Emerson Silva
author_facet Sugimoto, Michelle A.
de Jesus Amazonas da Silva, Márcia
Froede Brito, Larissa
dos Santos Borges, Rosivaldo
Amaral, Flávio Almeida
de Araujo Boleti, Ana Paula
Ordoñez, Maritza Echevarria
Carlos Tavares, Jose
Pires Sousa, Lirlandia
Lima, Emerson Silva
author_sort Sugimoto, Michelle A.
collection PubMed
description Inflammation is a reaction of the host to infectious or sterile stimuli and has the physiological purpose of restoring tissue homeostasis. However, uncontrolled or unresolved inflammation can lead to tissue damage, giving rise to a plethora of chronic inflammatory diseases, including metabolic syndrome and autoimmunity pathologies with eventual loss of organ function. Beta-nitrostyrene and its derivatives are known to have several biological activities, including anti-edema, vasorelaxant, antiplatelet, anti-inflammatory, and anticancer. However, few studies have been carried out regarding the anti-inflammatory effects of this class of compounds. Thereby, the aim of this study was to evaluate the anti-inflammatory activity of 1-nitro-2-phenylethene (NPe) using in vitro and in vivo assays. Firstly, the potential anti-inflammatory activity of NPe was evaluated by measuring TNF-α produced by human macrophages stimulated with lipopolysaccharide (LPS). NPe at non-toxic doses opposed the inflammatory effects induced by LPS stimulation, namely production of the inflammatory cytokine TNF-α and activation of NF-κB and ERK pathways (evaluated by phosphorylation of inhibitor of kappa B-alpha [IκB-α] and extracellular signal-regulated kinase 1/2 [ERK1/2], respectively). In a well-established model of acute pleurisy, pretreatment of LPS-challenged mice with NPe reduced neutrophil accumulation in the pleural cavity. This anti-inflammatory effect was associated with reduced activation of NF-κB and ERK1/2 pathways in NPe treated mice as compared to untreated animals. Notably, NPe was as effective as dexamethasone in both, reducing neutrophil accumulation and inhibiting ERK1/2 and IκB-α phosphorylation. Taken together, the results suggest a potential anti-inflammatory activity for NPe via inhibition of ERK1/2 and NF-κB pathways on leukocytes.
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spelling pubmed-61503672018-11-13 Anti-Inflammatory Potential of 1-Nitro-2-Phenylethylene Sugimoto, Michelle A. de Jesus Amazonas da Silva, Márcia Froede Brito, Larissa dos Santos Borges, Rosivaldo Amaral, Flávio Almeida de Araujo Boleti, Ana Paula Ordoñez, Maritza Echevarria Carlos Tavares, Jose Pires Sousa, Lirlandia Lima, Emerson Silva Molecules Article Inflammation is a reaction of the host to infectious or sterile stimuli and has the physiological purpose of restoring tissue homeostasis. However, uncontrolled or unresolved inflammation can lead to tissue damage, giving rise to a plethora of chronic inflammatory diseases, including metabolic syndrome and autoimmunity pathologies with eventual loss of organ function. Beta-nitrostyrene and its derivatives are known to have several biological activities, including anti-edema, vasorelaxant, antiplatelet, anti-inflammatory, and anticancer. However, few studies have been carried out regarding the anti-inflammatory effects of this class of compounds. Thereby, the aim of this study was to evaluate the anti-inflammatory activity of 1-nitro-2-phenylethene (NPe) using in vitro and in vivo assays. Firstly, the potential anti-inflammatory activity of NPe was evaluated by measuring TNF-α produced by human macrophages stimulated with lipopolysaccharide (LPS). NPe at non-toxic doses opposed the inflammatory effects induced by LPS stimulation, namely production of the inflammatory cytokine TNF-α and activation of NF-κB and ERK pathways (evaluated by phosphorylation of inhibitor of kappa B-alpha [IκB-α] and extracellular signal-regulated kinase 1/2 [ERK1/2], respectively). In a well-established model of acute pleurisy, pretreatment of LPS-challenged mice with NPe reduced neutrophil accumulation in the pleural cavity. This anti-inflammatory effect was associated with reduced activation of NF-κB and ERK1/2 pathways in NPe treated mice as compared to untreated animals. Notably, NPe was as effective as dexamethasone in both, reducing neutrophil accumulation and inhibiting ERK1/2 and IκB-α phosphorylation. Taken together, the results suggest a potential anti-inflammatory activity for NPe via inhibition of ERK1/2 and NF-κB pathways on leukocytes. MDPI 2017-11-15 /pmc/articles/PMC6150367/ /pubmed/29140265 http://dx.doi.org/10.3390/molecules22111977 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sugimoto, Michelle A.
de Jesus Amazonas da Silva, Márcia
Froede Brito, Larissa
dos Santos Borges, Rosivaldo
Amaral, Flávio Almeida
de Araujo Boleti, Ana Paula
Ordoñez, Maritza Echevarria
Carlos Tavares, Jose
Pires Sousa, Lirlandia
Lima, Emerson Silva
Anti-Inflammatory Potential of 1-Nitro-2-Phenylethylene
title Anti-Inflammatory Potential of 1-Nitro-2-Phenylethylene
title_full Anti-Inflammatory Potential of 1-Nitro-2-Phenylethylene
title_fullStr Anti-Inflammatory Potential of 1-Nitro-2-Phenylethylene
title_full_unstemmed Anti-Inflammatory Potential of 1-Nitro-2-Phenylethylene
title_short Anti-Inflammatory Potential of 1-Nitro-2-Phenylethylene
title_sort anti-inflammatory potential of 1-nitro-2-phenylethylene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6150367/
https://www.ncbi.nlm.nih.gov/pubmed/29140265
http://dx.doi.org/10.3390/molecules22111977
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