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Enhancing biomass and lipid accumulation in the microalgae Schizochytrium sp. by addition of fulvic acid and EDTA
Enhancing lipid productivity and reducing oxidative damage is essential for lipid overproduction in microalgae. In this study, addition of 20 mg/L fulvic acid (FA) resulted a 34.4% increase of lipid yield in Schizochytrium sp. Furthermore, the cooperative effect of FA and EDTA on cell growth and lip...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6150865/ https://www.ncbi.nlm.nih.gov/pubmed/30242564 http://dx.doi.org/10.1186/s13568-018-0681-5 |
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author | Sun, Xiao-Man Ren, Lu-Jing Ji, Xiao-Jun Huang, He |
author_facet | Sun, Xiao-Man Ren, Lu-Jing Ji, Xiao-Jun Huang, He |
author_sort | Sun, Xiao-Man |
collection | PubMed |
description | Enhancing lipid productivity and reducing oxidative damage is essential for lipid overproduction in microalgae. In this study, addition of 20 mg/L fulvic acid (FA) resulted a 34.4% increase of lipid yield in Schizochytrium sp. Furthermore, the cooperative effect of FA and EDTA on cell growth and lipid production was investigated. The combined addition of 20 mg/L FA and 1.0 g/L EDTA yielded a maximal cell dry weight of 130.7 g/L and lipid productivity of 1.16 g/L/h, representing 36.4% and threefold increase over the non-supplemented group, respectively. Moreover, compared with the non-supplemented group, the combined addition strategy exhibited overall lower levels of reactive oxygen species and malondialdehyde, which accompanied with 66.7% and 81.9% higher superoxide dismutase and catalase activity, respectively. Furthermore, a 24.1–37.1% increase of malic enzyme and 19.4–25.2% decrease of phosphoenolpyruvate carboxylase activity was observed during the entire fermentation stage (0–108 h). Results suggested that the combined addition strategy not only enhanced lipid accumulation, but also prevented the lipid peroxidation. |
format | Online Article Text |
id | pubmed-6150865 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-61508652018-10-09 Enhancing biomass and lipid accumulation in the microalgae Schizochytrium sp. by addition of fulvic acid and EDTA Sun, Xiao-Man Ren, Lu-Jing Ji, Xiao-Jun Huang, He AMB Express Original Article Enhancing lipid productivity and reducing oxidative damage is essential for lipid overproduction in microalgae. In this study, addition of 20 mg/L fulvic acid (FA) resulted a 34.4% increase of lipid yield in Schizochytrium sp. Furthermore, the cooperative effect of FA and EDTA on cell growth and lipid production was investigated. The combined addition of 20 mg/L FA and 1.0 g/L EDTA yielded a maximal cell dry weight of 130.7 g/L and lipid productivity of 1.16 g/L/h, representing 36.4% and threefold increase over the non-supplemented group, respectively. Moreover, compared with the non-supplemented group, the combined addition strategy exhibited overall lower levels of reactive oxygen species and malondialdehyde, which accompanied with 66.7% and 81.9% higher superoxide dismutase and catalase activity, respectively. Furthermore, a 24.1–37.1% increase of malic enzyme and 19.4–25.2% decrease of phosphoenolpyruvate carboxylase activity was observed during the entire fermentation stage (0–108 h). Results suggested that the combined addition strategy not only enhanced lipid accumulation, but also prevented the lipid peroxidation. Springer Berlin Heidelberg 2018-09-21 /pmc/articles/PMC6150865/ /pubmed/30242564 http://dx.doi.org/10.1186/s13568-018-0681-5 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Sun, Xiao-Man Ren, Lu-Jing Ji, Xiao-Jun Huang, He Enhancing biomass and lipid accumulation in the microalgae Schizochytrium sp. by addition of fulvic acid and EDTA |
title | Enhancing biomass and lipid accumulation in the microalgae Schizochytrium sp. by addition of fulvic acid and EDTA |
title_full | Enhancing biomass and lipid accumulation in the microalgae Schizochytrium sp. by addition of fulvic acid and EDTA |
title_fullStr | Enhancing biomass and lipid accumulation in the microalgae Schizochytrium sp. by addition of fulvic acid and EDTA |
title_full_unstemmed | Enhancing biomass and lipid accumulation in the microalgae Schizochytrium sp. by addition of fulvic acid and EDTA |
title_short | Enhancing biomass and lipid accumulation in the microalgae Schizochytrium sp. by addition of fulvic acid and EDTA |
title_sort | enhancing biomass and lipid accumulation in the microalgae schizochytrium sp. by addition of fulvic acid and edta |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6150865/ https://www.ncbi.nlm.nih.gov/pubmed/30242564 http://dx.doi.org/10.1186/s13568-018-0681-5 |
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