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Recent advances in graphene-based biosensor technology with applications in life sciences

Graphene’s unique physical structure, as well as its chemical and electrical properties, make it ideal for use in sensor technologies. In the past years, novel sensing platforms have been proposed with pristine and modified graphene with nanoparticles and polymers. Several of these platforms were us...

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Autores principales: Peña-Bahamonde, Janire, Nguyen, Hang N., Fanourakis, Sofia K., Rodrigues, Debora F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6150956/
https://www.ncbi.nlm.nih.gov/pubmed/30243292
http://dx.doi.org/10.1186/s12951-018-0400-z
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author Peña-Bahamonde, Janire
Nguyen, Hang N.
Fanourakis, Sofia K.
Rodrigues, Debora F.
author_facet Peña-Bahamonde, Janire
Nguyen, Hang N.
Fanourakis, Sofia K.
Rodrigues, Debora F.
author_sort Peña-Bahamonde, Janire
collection PubMed
description Graphene’s unique physical structure, as well as its chemical and electrical properties, make it ideal for use in sensor technologies. In the past years, novel sensing platforms have been proposed with pristine and modified graphene with nanoparticles and polymers. Several of these platforms were used to immobilize biomolecules, such as antibodies, DNA, and enzymes to create highly sensitive and selective biosensors. Strategies to attach these biomolecules onto the surface of graphene have been employed based on its chemical composition. These methods include covalent bonding, such as the coupling of the biomolecules via the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide reactions, and physisorption. In the literature, several detection methods are employed; however, the most common is electrochemical. The main reason for researchers to use this detection approach is because this method is simple, rapid and presents good sensitivity. These biosensors can be particularly useful in life sciences and medicine since in clinical practice, biosensors with high sensitivity and specificity can significantly enhance patient care, early diagnosis of diseases and pathogen detection. In this review, we will present the research conducted with antibodies, DNA molecules and, enzymes to develop biosensors that use graphene and its derivatives as scaffolds to produce effective biosensors able to detect and identify a variety of diseases, pathogens, and biomolecules linked to diseases.
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spelling pubmed-61509562018-09-26 Recent advances in graphene-based biosensor technology with applications in life sciences Peña-Bahamonde, Janire Nguyen, Hang N. Fanourakis, Sofia K. Rodrigues, Debora F. J Nanobiotechnology Review Graphene’s unique physical structure, as well as its chemical and electrical properties, make it ideal for use in sensor technologies. In the past years, novel sensing platforms have been proposed with pristine and modified graphene with nanoparticles and polymers. Several of these platforms were used to immobilize biomolecules, such as antibodies, DNA, and enzymes to create highly sensitive and selective biosensors. Strategies to attach these biomolecules onto the surface of graphene have been employed based on its chemical composition. These methods include covalent bonding, such as the coupling of the biomolecules via the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide reactions, and physisorption. In the literature, several detection methods are employed; however, the most common is electrochemical. The main reason for researchers to use this detection approach is because this method is simple, rapid and presents good sensitivity. These biosensors can be particularly useful in life sciences and medicine since in clinical practice, biosensors with high sensitivity and specificity can significantly enhance patient care, early diagnosis of diseases and pathogen detection. In this review, we will present the research conducted with antibodies, DNA molecules and, enzymes to develop biosensors that use graphene and its derivatives as scaffolds to produce effective biosensors able to detect and identify a variety of diseases, pathogens, and biomolecules linked to diseases. BioMed Central 2018-09-22 /pmc/articles/PMC6150956/ /pubmed/30243292 http://dx.doi.org/10.1186/s12951-018-0400-z Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Review
Peña-Bahamonde, Janire
Nguyen, Hang N.
Fanourakis, Sofia K.
Rodrigues, Debora F.
Recent advances in graphene-based biosensor technology with applications in life sciences
title Recent advances in graphene-based biosensor technology with applications in life sciences
title_full Recent advances in graphene-based biosensor technology with applications in life sciences
title_fullStr Recent advances in graphene-based biosensor technology with applications in life sciences
title_full_unstemmed Recent advances in graphene-based biosensor technology with applications in life sciences
title_short Recent advances in graphene-based biosensor technology with applications in life sciences
title_sort recent advances in graphene-based biosensor technology with applications in life sciences
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6150956/
https://www.ncbi.nlm.nih.gov/pubmed/30243292
http://dx.doi.org/10.1186/s12951-018-0400-z
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