A Fluorescent Coumarin-Based Probe for the Fast Detection of Cysteine with Live Cell Application

A new coumarin-based fluorescent probe, containing an allylic esters group, has been designed and synthesized for sensing cysteine in physiological pH. In this fluorescent probe, the coumarin was applied as the fluorophore and an allylic esters group was combined as both a fluorescence quencher and...

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Autores principales: Zeng, Rui-Feng, Lan, Jin-Shuai, Li, Xiao-Die, Liang, Hui-Fen, Liao, Yan, Lu, Ying-Jie, Zhang, Tong, Ding, Yue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151380/
https://www.ncbi.nlm.nih.gov/pubmed/28954423
http://dx.doi.org/10.3390/molecules22101618
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author Zeng, Rui-Feng
Lan, Jin-Shuai
Li, Xiao-Die
Liang, Hui-Fen
Liao, Yan
Lu, Ying-Jie
Zhang, Tong
Ding, Yue
author_facet Zeng, Rui-Feng
Lan, Jin-Shuai
Li, Xiao-Die
Liang, Hui-Fen
Liao, Yan
Lu, Ying-Jie
Zhang, Tong
Ding, Yue
author_sort Zeng, Rui-Feng
collection PubMed
description A new coumarin-based fluorescent probe, containing an allylic esters group, has been designed and synthesized for sensing cysteine in physiological pH. In this fluorescent probe, the coumarin was applied as the fluorophore and an allylic esters group was combined as both a fluorescence quencher and a recognition unit. The probe can selectively and sensitively detect cysteine (Cys) over homocysteine, glutathione, and other amino acids, and has a rapid response time of 30 min and a low detection limit of 47.7 nM. In addition, the probe could be applied for cell imaging with low cytotoxicity.
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spelling pubmed-61513802018-11-13 A Fluorescent Coumarin-Based Probe for the Fast Detection of Cysteine with Live Cell Application Zeng, Rui-Feng Lan, Jin-Shuai Li, Xiao-Die Liang, Hui-Fen Liao, Yan Lu, Ying-Jie Zhang, Tong Ding, Yue Molecules Article A new coumarin-based fluorescent probe, containing an allylic esters group, has been designed and synthesized for sensing cysteine in physiological pH. In this fluorescent probe, the coumarin was applied as the fluorophore and an allylic esters group was combined as both a fluorescence quencher and a recognition unit. The probe can selectively and sensitively detect cysteine (Cys) over homocysteine, glutathione, and other amino acids, and has a rapid response time of 30 min and a low detection limit of 47.7 nM. In addition, the probe could be applied for cell imaging with low cytotoxicity. MDPI 2017-09-26 /pmc/articles/PMC6151380/ /pubmed/28954423 http://dx.doi.org/10.3390/molecules22101618 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zeng, Rui-Feng
Lan, Jin-Shuai
Li, Xiao-Die
Liang, Hui-Fen
Liao, Yan
Lu, Ying-Jie
Zhang, Tong
Ding, Yue
A Fluorescent Coumarin-Based Probe for the Fast Detection of Cysteine with Live Cell Application
title A Fluorescent Coumarin-Based Probe for the Fast Detection of Cysteine with Live Cell Application
title_full A Fluorescent Coumarin-Based Probe for the Fast Detection of Cysteine with Live Cell Application
title_fullStr A Fluorescent Coumarin-Based Probe for the Fast Detection of Cysteine with Live Cell Application
title_full_unstemmed A Fluorescent Coumarin-Based Probe for the Fast Detection of Cysteine with Live Cell Application
title_short A Fluorescent Coumarin-Based Probe for the Fast Detection of Cysteine with Live Cell Application
title_sort fluorescent coumarin-based probe for the fast detection of cysteine with live cell application
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151380/
https://www.ncbi.nlm.nih.gov/pubmed/28954423
http://dx.doi.org/10.3390/molecules22101618
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