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Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts

Cudrania tricuspidata Bureau (Moraceae) shows numerous pharmacological effects and has been used in traditional herbal remedies for inflammation, gastritis, tumors, and liver diseases. However, no validated analytical method for the standardization and optimization of the biological properties of C....

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Autores principales: Song, Seung-Hui, Ki, Sung Hwan, Park, Dae-Hun, Moon, Hong-Seop, Lee, Chang-Dai, Yoon, In-Soo, Cho, Seung-Sik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151595/
https://www.ncbi.nlm.nih.gov/pubmed/28880226
http://dx.doi.org/10.3390/molecules22091489
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author Song, Seung-Hui
Ki, Sung Hwan
Park, Dae-Hun
Moon, Hong-Seop
Lee, Chang-Dai
Yoon, In-Soo
Cho, Seung-Sik
author_facet Song, Seung-Hui
Ki, Sung Hwan
Park, Dae-Hun
Moon, Hong-Seop
Lee, Chang-Dai
Yoon, In-Soo
Cho, Seung-Sik
author_sort Song, Seung-Hui
collection PubMed
description Cudrania tricuspidata Bureau (Moraceae) shows numerous pharmacological effects and has been used in traditional herbal remedies for inflammation, gastritis, tumors, and liver diseases. However, no validated analytical method for the standardization and optimization of the biological properties of C. tricuspidata preparations has been reported. We developed and validated a reverse-phase high-performance liquid chromatography (HPLC) method for the separation and quantification of active markers. Ethanolic extracts of C. tricuspidata leaves were prepared and evaluated for chemical profiles and biological activities. The 80% ethanolic extract demonstrated the greatest antioxidant activity and phenolic content, while the 100% ethanolic extract had the greatest total flavonoid content and xanthine oxidase (XO) inhibitory activity. The validated HPLC method confirmed that chlorogenic acid, rutin, and kaempferol were present in C. tricuspidata leaf extracts. We postulated that the antioxidant and anti-hyperuricemic/gout effects of C. tricuspidata extract could be attributed to these marker compounds. Our results suggested that the flavonoid-rich fraction of the leaf extract may be utilized for the treatment and prevention of hyperuricemia-related diseases, and the validated method and marker compounds could be applied for the quality control of C. tricuspidata preparations.
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spelling pubmed-61515952018-11-13 Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts Song, Seung-Hui Ki, Sung Hwan Park, Dae-Hun Moon, Hong-Seop Lee, Chang-Dai Yoon, In-Soo Cho, Seung-Sik Molecules Article Cudrania tricuspidata Bureau (Moraceae) shows numerous pharmacological effects and has been used in traditional herbal remedies for inflammation, gastritis, tumors, and liver diseases. However, no validated analytical method for the standardization and optimization of the biological properties of C. tricuspidata preparations has been reported. We developed and validated a reverse-phase high-performance liquid chromatography (HPLC) method for the separation and quantification of active markers. Ethanolic extracts of C. tricuspidata leaves were prepared and evaluated for chemical profiles and biological activities. The 80% ethanolic extract demonstrated the greatest antioxidant activity and phenolic content, while the 100% ethanolic extract had the greatest total flavonoid content and xanthine oxidase (XO) inhibitory activity. The validated HPLC method confirmed that chlorogenic acid, rutin, and kaempferol were present in C. tricuspidata leaf extracts. We postulated that the antioxidant and anti-hyperuricemic/gout effects of C. tricuspidata extract could be attributed to these marker compounds. Our results suggested that the flavonoid-rich fraction of the leaf extract may be utilized for the treatment and prevention of hyperuricemia-related diseases, and the validated method and marker compounds could be applied for the quality control of C. tricuspidata preparations. MDPI 2017-09-07 /pmc/articles/PMC6151595/ /pubmed/28880226 http://dx.doi.org/10.3390/molecules22091489 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Song, Seung-Hui
Ki, Sung Hwan
Park, Dae-Hun
Moon, Hong-Seop
Lee, Chang-Dai
Yoon, In-Soo
Cho, Seung-Sik
Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts
title Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts
title_full Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts
title_fullStr Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts
title_full_unstemmed Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts
title_short Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts
title_sort quantitative analysis, extraction optimization, and biological evaluation of cudrania tricuspidata leaf and fruit extracts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151595/
https://www.ncbi.nlm.nih.gov/pubmed/28880226
http://dx.doi.org/10.3390/molecules22091489
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