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The Small Glutathione Peroxidase Mimic 5P May Represent a New Strategy for the Treatment of Liver Cancer

Glutathione peroxidase (GPx) is an antioxidant protein containing selenium. Owing to the limitations of native GPx, considerable efforts have been made to develop GPx mimics. Here, a short 5-mer peptides (5P) was synthesized and characterized using matrix-assisted laser desorption ionization time-of...

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Autores principales: Yin, Juxin, Wang, Bingmei, Zhu, Xuejun, Qu, Xiaonan, Huang, Yi, Lv, Shaowu, Mu, Ying, Luo, Guimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151655/
https://www.ncbi.nlm.nih.gov/pubmed/28885589
http://dx.doi.org/10.3390/molecules22091495
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author Yin, Juxin
Wang, Bingmei
Zhu, Xuejun
Qu, Xiaonan
Huang, Yi
Lv, Shaowu
Mu, Ying
Luo, Guimin
author_facet Yin, Juxin
Wang, Bingmei
Zhu, Xuejun
Qu, Xiaonan
Huang, Yi
Lv, Shaowu
Mu, Ying
Luo, Guimin
author_sort Yin, Juxin
collection PubMed
description Glutathione peroxidase (GPx) is an antioxidant protein containing selenium. Owing to the limitations of native GPx, considerable efforts have been made to develop GPx mimics. Here, a short 5-mer peptides (5P) was synthesized and characterized using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Enzyme coupled assays were used to evaluate GPx activity. The cell viability and apoptosis of H22 cells were tested, and mice bearing H22 cell-derived tumors were used to determine the effects of 5P on tumor inhibition. In comparison with other enzyme models, 5P provided a suitable substrate with proper catalytic site positions, resulting in enhanced catalytic activity. In our mouse model, 5P showed excellent inhibition of tumor growth and improved immunity. In summary, our findings demonstrated the design and synthesis of the small 5P molecule, which inhibited tumor growth and improved immunity. Notably, 5P could inhibit tumor growth without affecting normal growth. Based on these advantages, the novel mimic may have several clinical applications.
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spelling pubmed-61516552018-11-13 The Small Glutathione Peroxidase Mimic 5P May Represent a New Strategy for the Treatment of Liver Cancer Yin, Juxin Wang, Bingmei Zhu, Xuejun Qu, Xiaonan Huang, Yi Lv, Shaowu Mu, Ying Luo, Guimin Molecules Article Glutathione peroxidase (GPx) is an antioxidant protein containing selenium. Owing to the limitations of native GPx, considerable efforts have been made to develop GPx mimics. Here, a short 5-mer peptides (5P) was synthesized and characterized using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Enzyme coupled assays were used to evaluate GPx activity. The cell viability and apoptosis of H22 cells were tested, and mice bearing H22 cell-derived tumors were used to determine the effects of 5P on tumor inhibition. In comparison with other enzyme models, 5P provided a suitable substrate with proper catalytic site positions, resulting in enhanced catalytic activity. In our mouse model, 5P showed excellent inhibition of tumor growth and improved immunity. In summary, our findings demonstrated the design and synthesis of the small 5P molecule, which inhibited tumor growth and improved immunity. Notably, 5P could inhibit tumor growth without affecting normal growth. Based on these advantages, the novel mimic may have several clinical applications. MDPI 2017-09-08 /pmc/articles/PMC6151655/ /pubmed/28885589 http://dx.doi.org/10.3390/molecules22091495 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yin, Juxin
Wang, Bingmei
Zhu, Xuejun
Qu, Xiaonan
Huang, Yi
Lv, Shaowu
Mu, Ying
Luo, Guimin
The Small Glutathione Peroxidase Mimic 5P May Represent a New Strategy for the Treatment of Liver Cancer
title The Small Glutathione Peroxidase Mimic 5P May Represent a New Strategy for the Treatment of Liver Cancer
title_full The Small Glutathione Peroxidase Mimic 5P May Represent a New Strategy for the Treatment of Liver Cancer
title_fullStr The Small Glutathione Peroxidase Mimic 5P May Represent a New Strategy for the Treatment of Liver Cancer
title_full_unstemmed The Small Glutathione Peroxidase Mimic 5P May Represent a New Strategy for the Treatment of Liver Cancer
title_short The Small Glutathione Peroxidase Mimic 5P May Represent a New Strategy for the Treatment of Liver Cancer
title_sort small glutathione peroxidase mimic 5p may represent a new strategy for the treatment of liver cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151655/
https://www.ncbi.nlm.nih.gov/pubmed/28885589
http://dx.doi.org/10.3390/molecules22091495
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