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The Effect of Pressure and Solvent on the Supercritical Fluid Chromatography Separation of Tocol Analogs in Palm Oil
There are six tocol analogs present in palm oil, namely α-tocopherol (α-T), α-tocomonoenol (α-T(1)), α-tocotrienol (α-T(3)), γ-tocotrienol (γ-T(3)), β-tocotrioenol (β-T(3)) and δ-tocotrienol (δ-T(3)). These analogs were difficult to separate chromatographically due to their similar structures, physi...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151672/ https://www.ncbi.nlm.nih.gov/pubmed/28850073 http://dx.doi.org/10.3390/molecules22091424 |
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author | Ng, Mei Han Kushairi, Ahmad |
author_facet | Ng, Mei Han Kushairi, Ahmad |
author_sort | Ng, Mei Han |
collection | PubMed |
description | There are six tocol analogs present in palm oil, namely α-tocopherol (α-T), α-tocomonoenol (α-T(1)), α-tocotrienol (α-T(3)), γ-tocotrienol (γ-T(3)), β-tocotrioenol (β-T(3)) and δ-tocotrienol (δ-T(3)). These analogs were difficult to separate chromatographically due to their similar structures, physical and chemical properties. This paper reports on the effect of pressure and injection solvent on the separation of the tocol analogs in palm oil. Supercritical CO(2) modified with ethanol was used as the mobile phase. Both total elution time and resolution of the tocol analogs decreased with increased pressure. Ethanol as an injection solvent resulted in peak broadening of the analogs within the entire pressure range studied. Solvents with an eluent strength of 3.4 or less were more suitable for use as injecting solvents. |
format | Online Article Text |
id | pubmed-6151672 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61516722018-11-13 The Effect of Pressure and Solvent on the Supercritical Fluid Chromatography Separation of Tocol Analogs in Palm Oil Ng, Mei Han Kushairi, Ahmad Molecules Article There are six tocol analogs present in palm oil, namely α-tocopherol (α-T), α-tocomonoenol (α-T(1)), α-tocotrienol (α-T(3)), γ-tocotrienol (γ-T(3)), β-tocotrioenol (β-T(3)) and δ-tocotrienol (δ-T(3)). These analogs were difficult to separate chromatographically due to their similar structures, physical and chemical properties. This paper reports on the effect of pressure and injection solvent on the separation of the tocol analogs in palm oil. Supercritical CO(2) modified with ethanol was used as the mobile phase. Both total elution time and resolution of the tocol analogs decreased with increased pressure. Ethanol as an injection solvent resulted in peak broadening of the analogs within the entire pressure range studied. Solvents with an eluent strength of 3.4 or less were more suitable for use as injecting solvents. MDPI 2017-08-29 /pmc/articles/PMC6151672/ /pubmed/28850073 http://dx.doi.org/10.3390/molecules22091424 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ng, Mei Han Kushairi, Ahmad The Effect of Pressure and Solvent on the Supercritical Fluid Chromatography Separation of Tocol Analogs in Palm Oil |
title | The Effect of Pressure and Solvent on the Supercritical Fluid Chromatography Separation of Tocol Analogs in Palm Oil |
title_full | The Effect of Pressure and Solvent on the Supercritical Fluid Chromatography Separation of Tocol Analogs in Palm Oil |
title_fullStr | The Effect of Pressure and Solvent on the Supercritical Fluid Chromatography Separation of Tocol Analogs in Palm Oil |
title_full_unstemmed | The Effect of Pressure and Solvent on the Supercritical Fluid Chromatography Separation of Tocol Analogs in Palm Oil |
title_short | The Effect of Pressure and Solvent on the Supercritical Fluid Chromatography Separation of Tocol Analogs in Palm Oil |
title_sort | effect of pressure and solvent on the supercritical fluid chromatography separation of tocol analogs in palm oil |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151672/ https://www.ncbi.nlm.nih.gov/pubmed/28850073 http://dx.doi.org/10.3390/molecules22091424 |
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