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N-Halamine Biocidal Materials with Superior Antimicrobial Efficacies for Wound Dressings

This work demonstrated the successful application of N-halamine technology for wound dressings rendered antimicrobial by facile and inexpensive processes. Four N-halamine compounds, which possess different functional groups and chemistry, were synthesized. The N-halamine compounds, which contained o...

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Autores principales: Demir, Buket, Broughton, Roy M., Qiao, Mingyu, Huang, Tung-Shi, Worley, S. D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151735/
https://www.ncbi.nlm.nih.gov/pubmed/28934124
http://dx.doi.org/10.3390/molecules22101582
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author Demir, Buket
Broughton, Roy M.
Qiao, Mingyu
Huang, Tung-Shi
Worley, S. D.
author_facet Demir, Buket
Broughton, Roy M.
Qiao, Mingyu
Huang, Tung-Shi
Worley, S. D.
author_sort Demir, Buket
collection PubMed
description This work demonstrated the successful application of N-halamine technology for wound dressings rendered antimicrobial by facile and inexpensive processes. Four N-halamine compounds, which possess different functional groups and chemistry, were synthesized. The N-halamine compounds, which contained oxidative chlorine, the source of antimicrobial activity, were impregnated into or coated onto standard non-antimicrobial wound dressings. N-halamine-employed wound dressings inactivated about 6 to 7 logs of Staphylococcus aureus and Pseudomonas aeruginosa bacteria in brief periods of contact time. Moreover, the N-halamine-modified wound dressings showed superior antimicrobial efficacies when compared to commercially available silver wound dressings. Zone of inhibition tests revealed that there was no significant leaching of the oxidative chlorine from the materials, and inactivation of bacteria occurred by direct contact. Shelf life stability tests showed that the dressings were stable to loss of oxidative chlorine when they were stored for 6 months in dark environmental conditions. They also remained stable under florescent lighting for up to 2 months of storage. They could be stored in opaque packaging to improve their shelf life stabilities. In vitro skin irritation testing was performed using a three-dimensional human reconstructed tissue model (EpiDerm™). No potential skin irritation was observed. In vitro cytocompatibility was also evaluated. These results indicate that N-halamine wound dressings potentially can be employed to prevent infections, while at the same time improving the healing process by eliminating undesired bacterial growth.
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spelling pubmed-61517352018-11-13 N-Halamine Biocidal Materials with Superior Antimicrobial Efficacies for Wound Dressings Demir, Buket Broughton, Roy M. Qiao, Mingyu Huang, Tung-Shi Worley, S. D. Molecules Article This work demonstrated the successful application of N-halamine technology for wound dressings rendered antimicrobial by facile and inexpensive processes. Four N-halamine compounds, which possess different functional groups and chemistry, were synthesized. The N-halamine compounds, which contained oxidative chlorine, the source of antimicrobial activity, were impregnated into or coated onto standard non-antimicrobial wound dressings. N-halamine-employed wound dressings inactivated about 6 to 7 logs of Staphylococcus aureus and Pseudomonas aeruginosa bacteria in brief periods of contact time. Moreover, the N-halamine-modified wound dressings showed superior antimicrobial efficacies when compared to commercially available silver wound dressings. Zone of inhibition tests revealed that there was no significant leaching of the oxidative chlorine from the materials, and inactivation of bacteria occurred by direct contact. Shelf life stability tests showed that the dressings were stable to loss of oxidative chlorine when they were stored for 6 months in dark environmental conditions. They also remained stable under florescent lighting for up to 2 months of storage. They could be stored in opaque packaging to improve their shelf life stabilities. In vitro skin irritation testing was performed using a three-dimensional human reconstructed tissue model (EpiDerm™). No potential skin irritation was observed. In vitro cytocompatibility was also evaluated. These results indicate that N-halamine wound dressings potentially can be employed to prevent infections, while at the same time improving the healing process by eliminating undesired bacterial growth. MDPI 2017-09-21 /pmc/articles/PMC6151735/ /pubmed/28934124 http://dx.doi.org/10.3390/molecules22101582 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Demir, Buket
Broughton, Roy M.
Qiao, Mingyu
Huang, Tung-Shi
Worley, S. D.
N-Halamine Biocidal Materials with Superior Antimicrobial Efficacies for Wound Dressings
title N-Halamine Biocidal Materials with Superior Antimicrobial Efficacies for Wound Dressings
title_full N-Halamine Biocidal Materials with Superior Antimicrobial Efficacies for Wound Dressings
title_fullStr N-Halamine Biocidal Materials with Superior Antimicrobial Efficacies for Wound Dressings
title_full_unstemmed N-Halamine Biocidal Materials with Superior Antimicrobial Efficacies for Wound Dressings
title_short N-Halamine Biocidal Materials with Superior Antimicrobial Efficacies for Wound Dressings
title_sort n-halamine biocidal materials with superior antimicrobial efficacies for wound dressings
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151735/
https://www.ncbi.nlm.nih.gov/pubmed/28934124
http://dx.doi.org/10.3390/molecules22101582
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