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Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis

Ophiocordyceps xuefengensis, a recently described species of Ophiocordyceps that is associated with the larvae of Phassus nodus (Hepialidae) in the living root or trunk of the medicinal plant Clerodendrum cyrtophyllum, is the largest known Cordyceps species and is recognized as a desirable alternati...

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Autores principales: Zou, Juan, Wu, Ling, He, Zheng-Mi, Zhang, Ping, Chen, Zuo-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151754/
https://www.ncbi.nlm.nih.gov/pubmed/28891979
http://dx.doi.org/10.3390/molecules22091530
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author Zou, Juan
Wu, Ling
He, Zheng-Mi
Zhang, Ping
Chen, Zuo-Hong
author_facet Zou, Juan
Wu, Ling
He, Zheng-Mi
Zhang, Ping
Chen, Zuo-Hong
author_sort Zou, Juan
collection PubMed
description Ophiocordyceps xuefengensis, a recently described species of Ophiocordyceps that is associated with the larvae of Phassus nodus (Hepialidae) in the living root or trunk of the medicinal plant Clerodendrum cyrtophyllum, is the largest known Cordyceps species and is recognized as a desirable alternative for natural Ophiocordyceps sinensis. This study investigated the main nucleosides and nucleobases in natural and cultured Ophiocordyceps xuefengensis. The contents of the nucleosides and nucleobases in the natural and cultured samples were determined by reverse phase HPLC. The highest concentration of adenosine was found in the natural fruit body and the cultured stroma, with almost no adenosine in the cadaver of Phassus nodus. The contents of adenine, guanosine, uridine and uracil in the cultured mycelium were significantly higher than those in the natural sample. Inosine was only detected in the natural samples. Thymidine and 2-deoxyadenosine were only found in the cadaver of Phassus nodus. Cordycepin was not detected in the five samples examined. These results suggested that the cultured mycelium and cultured stroma of Ophiocordyceps xuefengensis might be a promising substitute for natural O. xuefengensis.
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spelling pubmed-61517542018-11-13 Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis Zou, Juan Wu, Ling He, Zheng-Mi Zhang, Ping Chen, Zuo-Hong Molecules Article Ophiocordyceps xuefengensis, a recently described species of Ophiocordyceps that is associated with the larvae of Phassus nodus (Hepialidae) in the living root or trunk of the medicinal plant Clerodendrum cyrtophyllum, is the largest known Cordyceps species and is recognized as a desirable alternative for natural Ophiocordyceps sinensis. This study investigated the main nucleosides and nucleobases in natural and cultured Ophiocordyceps xuefengensis. The contents of the nucleosides and nucleobases in the natural and cultured samples were determined by reverse phase HPLC. The highest concentration of adenosine was found in the natural fruit body and the cultured stroma, with almost no adenosine in the cadaver of Phassus nodus. The contents of adenine, guanosine, uridine and uracil in the cultured mycelium were significantly higher than those in the natural sample. Inosine was only detected in the natural samples. Thymidine and 2-deoxyadenosine were only found in the cadaver of Phassus nodus. Cordycepin was not detected in the five samples examined. These results suggested that the cultured mycelium and cultured stroma of Ophiocordyceps xuefengensis might be a promising substitute for natural O. xuefengensis. MDPI 2017-09-11 /pmc/articles/PMC6151754/ /pubmed/28891979 http://dx.doi.org/10.3390/molecules22091530 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zou, Juan
Wu, Ling
He, Zheng-Mi
Zhang, Ping
Chen, Zuo-Hong
Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis
title Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis
title_full Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis
title_fullStr Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis
title_full_unstemmed Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis
title_short Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis
title_sort determination of the main nucleosides and nucleobases in natural and cultured ophiocordyceps xuefengensis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151754/
https://www.ncbi.nlm.nih.gov/pubmed/28891979
http://dx.doi.org/10.3390/molecules22091530
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