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Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis
Ophiocordyceps xuefengensis, a recently described species of Ophiocordyceps that is associated with the larvae of Phassus nodus (Hepialidae) in the living root or trunk of the medicinal plant Clerodendrum cyrtophyllum, is the largest known Cordyceps species and is recognized as a desirable alternati...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151754/ https://www.ncbi.nlm.nih.gov/pubmed/28891979 http://dx.doi.org/10.3390/molecules22091530 |
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author | Zou, Juan Wu, Ling He, Zheng-Mi Zhang, Ping Chen, Zuo-Hong |
author_facet | Zou, Juan Wu, Ling He, Zheng-Mi Zhang, Ping Chen, Zuo-Hong |
author_sort | Zou, Juan |
collection | PubMed |
description | Ophiocordyceps xuefengensis, a recently described species of Ophiocordyceps that is associated with the larvae of Phassus nodus (Hepialidae) in the living root or trunk of the medicinal plant Clerodendrum cyrtophyllum, is the largest known Cordyceps species and is recognized as a desirable alternative for natural Ophiocordyceps sinensis. This study investigated the main nucleosides and nucleobases in natural and cultured Ophiocordyceps xuefengensis. The contents of the nucleosides and nucleobases in the natural and cultured samples were determined by reverse phase HPLC. The highest concentration of adenosine was found in the natural fruit body and the cultured stroma, with almost no adenosine in the cadaver of Phassus nodus. The contents of adenine, guanosine, uridine and uracil in the cultured mycelium were significantly higher than those in the natural sample. Inosine was only detected in the natural samples. Thymidine and 2-deoxyadenosine were only found in the cadaver of Phassus nodus. Cordycepin was not detected in the five samples examined. These results suggested that the cultured mycelium and cultured stroma of Ophiocordyceps xuefengensis might be a promising substitute for natural O. xuefengensis. |
format | Online Article Text |
id | pubmed-6151754 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61517542018-11-13 Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis Zou, Juan Wu, Ling He, Zheng-Mi Zhang, Ping Chen, Zuo-Hong Molecules Article Ophiocordyceps xuefengensis, a recently described species of Ophiocordyceps that is associated with the larvae of Phassus nodus (Hepialidae) in the living root or trunk of the medicinal plant Clerodendrum cyrtophyllum, is the largest known Cordyceps species and is recognized as a desirable alternative for natural Ophiocordyceps sinensis. This study investigated the main nucleosides and nucleobases in natural and cultured Ophiocordyceps xuefengensis. The contents of the nucleosides and nucleobases in the natural and cultured samples were determined by reverse phase HPLC. The highest concentration of adenosine was found in the natural fruit body and the cultured stroma, with almost no adenosine in the cadaver of Phassus nodus. The contents of adenine, guanosine, uridine and uracil in the cultured mycelium were significantly higher than those in the natural sample. Inosine was only detected in the natural samples. Thymidine and 2-deoxyadenosine were only found in the cadaver of Phassus nodus. Cordycepin was not detected in the five samples examined. These results suggested that the cultured mycelium and cultured stroma of Ophiocordyceps xuefengensis might be a promising substitute for natural O. xuefengensis. MDPI 2017-09-11 /pmc/articles/PMC6151754/ /pubmed/28891979 http://dx.doi.org/10.3390/molecules22091530 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zou, Juan Wu, Ling He, Zheng-Mi Zhang, Ping Chen, Zuo-Hong Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis |
title | Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps
xuefengensis |
title_full | Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps
xuefengensis |
title_fullStr | Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps
xuefengensis |
title_full_unstemmed | Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps
xuefengensis |
title_short | Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps
xuefengensis |
title_sort | determination of the main nucleosides and nucleobases in natural and cultured ophiocordyceps
xuefengensis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151754/ https://www.ncbi.nlm.nih.gov/pubmed/28891979 http://dx.doi.org/10.3390/molecules22091530 |
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