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Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides
Silica nanoparticles were functionalized with immobilized molecular bait, Cibacron Blue, and a porous polymeric bis-acrylamide shell. These nanoparticles represent a new alternative to capture low molecular weight (LMW) proteins/peptides, that might be potential biomarkers. Functionalized core-shell...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151791/ https://www.ncbi.nlm.nih.gov/pubmed/29023404 http://dx.doi.org/10.3390/molecules22101712 |
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author | Hernandez-Leon, Sergio G. Sarabia-Sainz, Jose Andre-i Montfort, Gabriela Ramos-Clamont Guzman-Partida, Ana M. Robles-Burgueño, Maria del Refugio Vazquez-Moreno, Luz |
author_facet | Hernandez-Leon, Sergio G. Sarabia-Sainz, Jose Andre-i Montfort, Gabriela Ramos-Clamont Guzman-Partida, Ana M. Robles-Burgueño, Maria del Refugio Vazquez-Moreno, Luz |
author_sort | Hernandez-Leon, Sergio G. |
collection | PubMed |
description | Silica nanoparticles were functionalized with immobilized molecular bait, Cibacron Blue, and a porous polymeric bis-acrylamide shell. These nanoparticles represent a new alternative to capture low molecular weight (LMW) proteins/peptides, that might be potential biomarkers. Functionalized core-shell silica nanoparticles (FCSNP) presented a size distribution of 243.9 ± 11.6 nm and an estimated surface charge of −38.1 ± 0.9 mV. The successful attachment of compounds at every stage of synthesis was evidenced by ATR-FTIR. The capture of model peptides was determined by mass spectrometry, indicating that only the peptide with a long sequence of hydrophobic amino acids (alpha zein 34-mer) interacted with the molecular bait. FCSNP excluded the high molecular weight protein (HMW), BSA, and captured LMW proteins (myoglobin and aprotinin), as evidenced by SDS-PAGE. Functionalization of nanoparticles with Cibacron Blue was crucial to capture these molecules. FCSNP were stable after twelve months of storage and maintained a capacity of 3.1–3.4 µg/mg. |
format | Online Article Text |
id | pubmed-6151791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61517912018-11-13 Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides Hernandez-Leon, Sergio G. Sarabia-Sainz, Jose Andre-i Montfort, Gabriela Ramos-Clamont Guzman-Partida, Ana M. Robles-Burgueño, Maria del Refugio Vazquez-Moreno, Luz Molecules Article Silica nanoparticles were functionalized with immobilized molecular bait, Cibacron Blue, and a porous polymeric bis-acrylamide shell. These nanoparticles represent a new alternative to capture low molecular weight (LMW) proteins/peptides, that might be potential biomarkers. Functionalized core-shell silica nanoparticles (FCSNP) presented a size distribution of 243.9 ± 11.6 nm and an estimated surface charge of −38.1 ± 0.9 mV. The successful attachment of compounds at every stage of synthesis was evidenced by ATR-FTIR. The capture of model peptides was determined by mass spectrometry, indicating that only the peptide with a long sequence of hydrophobic amino acids (alpha zein 34-mer) interacted with the molecular bait. FCSNP excluded the high molecular weight protein (HMW), BSA, and captured LMW proteins (myoglobin and aprotinin), as evidenced by SDS-PAGE. Functionalization of nanoparticles with Cibacron Blue was crucial to capture these molecules. FCSNP were stable after twelve months of storage and maintained a capacity of 3.1–3.4 µg/mg. MDPI 2017-10-12 /pmc/articles/PMC6151791/ /pubmed/29023404 http://dx.doi.org/10.3390/molecules22101712 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hernandez-Leon, Sergio G. Sarabia-Sainz, Jose Andre-i Montfort, Gabriela Ramos-Clamont Guzman-Partida, Ana M. Robles-Burgueño, Maria del Refugio Vazquez-Moreno, Luz Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides |
title | Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides |
title_full | Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides |
title_fullStr | Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides |
title_full_unstemmed | Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides |
title_short | Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides |
title_sort | novel synthesis of core-shell silica nanoparticles for the capture of low molecular weight proteins and peptides |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151791/ https://www.ncbi.nlm.nih.gov/pubmed/29023404 http://dx.doi.org/10.3390/molecules22101712 |
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