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Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides

Silica nanoparticles were functionalized with immobilized molecular bait, Cibacron Blue, and a porous polymeric bis-acrylamide shell. These nanoparticles represent a new alternative to capture low molecular weight (LMW) proteins/peptides, that might be potential biomarkers. Functionalized core-shell...

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Autores principales: Hernandez-Leon, Sergio G., Sarabia-Sainz, Jose Andre-i, Montfort, Gabriela Ramos-Clamont, Guzman-Partida, Ana M., Robles-Burgueño, Maria del Refugio, Vazquez-Moreno, Luz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151791/
https://www.ncbi.nlm.nih.gov/pubmed/29023404
http://dx.doi.org/10.3390/molecules22101712
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author Hernandez-Leon, Sergio G.
Sarabia-Sainz, Jose Andre-i
Montfort, Gabriela Ramos-Clamont
Guzman-Partida, Ana M.
Robles-Burgueño, Maria del Refugio
Vazquez-Moreno, Luz
author_facet Hernandez-Leon, Sergio G.
Sarabia-Sainz, Jose Andre-i
Montfort, Gabriela Ramos-Clamont
Guzman-Partida, Ana M.
Robles-Burgueño, Maria del Refugio
Vazquez-Moreno, Luz
author_sort Hernandez-Leon, Sergio G.
collection PubMed
description Silica nanoparticles were functionalized with immobilized molecular bait, Cibacron Blue, and a porous polymeric bis-acrylamide shell. These nanoparticles represent a new alternative to capture low molecular weight (LMW) proteins/peptides, that might be potential biomarkers. Functionalized core-shell silica nanoparticles (FCSNP) presented a size distribution of 243.9 ± 11.6 nm and an estimated surface charge of −38.1 ± 0.9 mV. The successful attachment of compounds at every stage of synthesis was evidenced by ATR-FTIR. The capture of model peptides was determined by mass spectrometry, indicating that only the peptide with a long sequence of hydrophobic amino acids (alpha zein 34-mer) interacted with the molecular bait. FCSNP excluded the high molecular weight protein (HMW), BSA, and captured LMW proteins (myoglobin and aprotinin), as evidenced by SDS-PAGE. Functionalization of nanoparticles with Cibacron Blue was crucial to capture these molecules. FCSNP were stable after twelve months of storage and maintained a capacity of 3.1–3.4 µg/mg.
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spelling pubmed-61517912018-11-13 Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides Hernandez-Leon, Sergio G. Sarabia-Sainz, Jose Andre-i Montfort, Gabriela Ramos-Clamont Guzman-Partida, Ana M. Robles-Burgueño, Maria del Refugio Vazquez-Moreno, Luz Molecules Article Silica nanoparticles were functionalized with immobilized molecular bait, Cibacron Blue, and a porous polymeric bis-acrylamide shell. These nanoparticles represent a new alternative to capture low molecular weight (LMW) proteins/peptides, that might be potential biomarkers. Functionalized core-shell silica nanoparticles (FCSNP) presented a size distribution of 243.9 ± 11.6 nm and an estimated surface charge of −38.1 ± 0.9 mV. The successful attachment of compounds at every stage of synthesis was evidenced by ATR-FTIR. The capture of model peptides was determined by mass spectrometry, indicating that only the peptide with a long sequence of hydrophobic amino acids (alpha zein 34-mer) interacted with the molecular bait. FCSNP excluded the high molecular weight protein (HMW), BSA, and captured LMW proteins (myoglobin and aprotinin), as evidenced by SDS-PAGE. Functionalization of nanoparticles with Cibacron Blue was crucial to capture these molecules. FCSNP were stable after twelve months of storage and maintained a capacity of 3.1–3.4 µg/mg. MDPI 2017-10-12 /pmc/articles/PMC6151791/ /pubmed/29023404 http://dx.doi.org/10.3390/molecules22101712 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hernandez-Leon, Sergio G.
Sarabia-Sainz, Jose Andre-i
Montfort, Gabriela Ramos-Clamont
Guzman-Partida, Ana M.
Robles-Burgueño, Maria del Refugio
Vazquez-Moreno, Luz
Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides
title Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides
title_full Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides
title_fullStr Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides
title_full_unstemmed Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides
title_short Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides
title_sort novel synthesis of core-shell silica nanoparticles for the capture of low molecular weight proteins and peptides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151791/
https://www.ncbi.nlm.nih.gov/pubmed/29023404
http://dx.doi.org/10.3390/molecules22101712
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