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The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells
Melatonin (MLT) plays an important role in regulating the physiological cycle of seasonal breeding animals. Melatonin receptor I (MT1) is effectively expressed in the cambium layer of deer antler. However, the function and metabolic mechanism of MLT/MT1 signaling in the mesenchymal cells of sika dee...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151843/ https://www.ncbi.nlm.nih.gov/pubmed/29065543 http://dx.doi.org/10.3390/molecules22101793 |
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author | Yang, Feifei He, Changjiu Sun, Xuyang Wang, Jing Luo, Can Liu, Guoshi Yang, Liguo Xiong, Jiajun Huo, Lijun |
author_facet | Yang, Feifei He, Changjiu Sun, Xuyang Wang, Jing Luo, Can Liu, Guoshi Yang, Liguo Xiong, Jiajun Huo, Lijun |
author_sort | Yang, Feifei |
collection | PubMed |
description | Melatonin (MLT) plays an important role in regulating the physiological cycle of seasonal breeding animals. Melatonin receptor I (MT1) is effectively expressed in the cambium layer of deer antler. However, the function and metabolic mechanism of MLT/MT1 signaling in the mesenchymal cells of sika deer remain to be further elucidated. In this work, we detected the effects of MLT/MT1 signaling on mesenchymal cells proliferation and the interaction between MLT/MT1 and IGF1/IGF1-R signaling. The results show that (1) deer antler mesenchymal cells actually express MT1; (2) exogenous melatonin significantly promotes mesenchymal cells proliferation, while MT1 knock-down significantly impairs the positive effects of melatonin; and (3) melatonin significantly enhanced IGF1/IGF1-R signaling, as both the expression of IGF1 and IGF-1R increased, while MT1 knock-down significantly decreased IGF1-R expression and IGF1 synthesis. In summary, these data verified that MLT/MT1 signaling plays a crucial role in antler mesenchymal proliferation, which may be mediated by IGF1/IGF1-R. |
format | Online Article Text |
id | pubmed-6151843 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61518432018-11-13 The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells Yang, Feifei He, Changjiu Sun, Xuyang Wang, Jing Luo, Can Liu, Guoshi Yang, Liguo Xiong, Jiajun Huo, Lijun Molecules Article Melatonin (MLT) plays an important role in regulating the physiological cycle of seasonal breeding animals. Melatonin receptor I (MT1) is effectively expressed in the cambium layer of deer antler. However, the function and metabolic mechanism of MLT/MT1 signaling in the mesenchymal cells of sika deer remain to be further elucidated. In this work, we detected the effects of MLT/MT1 signaling on mesenchymal cells proliferation and the interaction between MLT/MT1 and IGF1/IGF1-R signaling. The results show that (1) deer antler mesenchymal cells actually express MT1; (2) exogenous melatonin significantly promotes mesenchymal cells proliferation, while MT1 knock-down significantly impairs the positive effects of melatonin; and (3) melatonin significantly enhanced IGF1/IGF1-R signaling, as both the expression of IGF1 and IGF-1R increased, while MT1 knock-down significantly decreased IGF1-R expression and IGF1 synthesis. In summary, these data verified that MLT/MT1 signaling plays a crucial role in antler mesenchymal proliferation, which may be mediated by IGF1/IGF1-R. MDPI 2017-10-23 /pmc/articles/PMC6151843/ /pubmed/29065543 http://dx.doi.org/10.3390/molecules22101793 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Feifei He, Changjiu Sun, Xuyang Wang, Jing Luo, Can Liu, Guoshi Yang, Liguo Xiong, Jiajun Huo, Lijun The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells |
title | The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells |
title_full | The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells |
title_fullStr | The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells |
title_full_unstemmed | The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells |
title_short | The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells |
title_sort | regulatory mechanism of mlt/mt1 signaling on the growth of antler mesenchymal cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151843/ https://www.ncbi.nlm.nih.gov/pubmed/29065543 http://dx.doi.org/10.3390/molecules22101793 |
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