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The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells

Melatonin (MLT) plays an important role in regulating the physiological cycle of seasonal breeding animals. Melatonin receptor I (MT1) is effectively expressed in the cambium layer of deer antler. However, the function and metabolic mechanism of MLT/MT1 signaling in the mesenchymal cells of sika dee...

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Autores principales: Yang, Feifei, He, Changjiu, Sun, Xuyang, Wang, Jing, Luo, Can, Liu, Guoshi, Yang, Liguo, Xiong, Jiajun, Huo, Lijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151843/
https://www.ncbi.nlm.nih.gov/pubmed/29065543
http://dx.doi.org/10.3390/molecules22101793
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author Yang, Feifei
He, Changjiu
Sun, Xuyang
Wang, Jing
Luo, Can
Liu, Guoshi
Yang, Liguo
Xiong, Jiajun
Huo, Lijun
author_facet Yang, Feifei
He, Changjiu
Sun, Xuyang
Wang, Jing
Luo, Can
Liu, Guoshi
Yang, Liguo
Xiong, Jiajun
Huo, Lijun
author_sort Yang, Feifei
collection PubMed
description Melatonin (MLT) plays an important role in regulating the physiological cycle of seasonal breeding animals. Melatonin receptor I (MT1) is effectively expressed in the cambium layer of deer antler. However, the function and metabolic mechanism of MLT/MT1 signaling in the mesenchymal cells of sika deer remain to be further elucidated. In this work, we detected the effects of MLT/MT1 signaling on mesenchymal cells proliferation and the interaction between MLT/MT1 and IGF1/IGF1-R signaling. The results show that (1) deer antler mesenchymal cells actually express MT1; (2) exogenous melatonin significantly promotes mesenchymal cells proliferation, while MT1 knock-down significantly impairs the positive effects of melatonin; and (3) melatonin significantly enhanced IGF1/IGF1-R signaling, as both the expression of IGF1 and IGF-1R increased, while MT1 knock-down significantly decreased IGF1-R expression and IGF1 synthesis. In summary, these data verified that MLT/MT1 signaling plays a crucial role in antler mesenchymal proliferation, which may be mediated by IGF1/IGF1-R.
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spelling pubmed-61518432018-11-13 The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells Yang, Feifei He, Changjiu Sun, Xuyang Wang, Jing Luo, Can Liu, Guoshi Yang, Liguo Xiong, Jiajun Huo, Lijun Molecules Article Melatonin (MLT) plays an important role in regulating the physiological cycle of seasonal breeding animals. Melatonin receptor I (MT1) is effectively expressed in the cambium layer of deer antler. However, the function and metabolic mechanism of MLT/MT1 signaling in the mesenchymal cells of sika deer remain to be further elucidated. In this work, we detected the effects of MLT/MT1 signaling on mesenchymal cells proliferation and the interaction between MLT/MT1 and IGF1/IGF1-R signaling. The results show that (1) deer antler mesenchymal cells actually express MT1; (2) exogenous melatonin significantly promotes mesenchymal cells proliferation, while MT1 knock-down significantly impairs the positive effects of melatonin; and (3) melatonin significantly enhanced IGF1/IGF1-R signaling, as both the expression of IGF1 and IGF-1R increased, while MT1 knock-down significantly decreased IGF1-R expression and IGF1 synthesis. In summary, these data verified that MLT/MT1 signaling plays a crucial role in antler mesenchymal proliferation, which may be mediated by IGF1/IGF1-R. MDPI 2017-10-23 /pmc/articles/PMC6151843/ /pubmed/29065543 http://dx.doi.org/10.3390/molecules22101793 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Feifei
He, Changjiu
Sun, Xuyang
Wang, Jing
Luo, Can
Liu, Guoshi
Yang, Liguo
Xiong, Jiajun
Huo, Lijun
The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells
title The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells
title_full The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells
title_fullStr The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells
title_full_unstemmed The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells
title_short The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells
title_sort regulatory mechanism of mlt/mt1 signaling on the growth of antler mesenchymal cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151843/
https://www.ncbi.nlm.nih.gov/pubmed/29065543
http://dx.doi.org/10.3390/molecules22101793
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