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Molecular Cloning and Characterization of PnbHLH1 Transcription Factor in Panax notoginseng
Panax notoginseng has been extensively used as a traditional Chinese medicine. In the current study, molecular cloning and characterization of PnbHLH1 transcription factor were explored in Panax notoginseng. The full length of the PnbHLH1 gene obtained by splicing was 1430 bp, encoding 321 amino aci...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152055/ https://www.ncbi.nlm.nih.gov/pubmed/28758911 http://dx.doi.org/10.3390/molecules22081268 |
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author | Zhang, Xiang Ge, Feng Deng, Bing Shah, Taif Huang, Zhuangjia Liu, Diqiu Chen, Chaoyin |
author_facet | Zhang, Xiang Ge, Feng Deng, Bing Shah, Taif Huang, Zhuangjia Liu, Diqiu Chen, Chaoyin |
author_sort | Zhang, Xiang |
collection | PubMed |
description | Panax notoginseng has been extensively used as a traditional Chinese medicine. In the current study, molecular cloning and characterization of PnbHLH1 transcription factor were explored in Panax notoginseng. The full length of the PnbHLH1 gene obtained by splicing was 1430 bp, encoding 321 amino acids. Prokaryotic expression vector pET-28a-PnbHLH1 was constructed and transferred into the BL21 prokaryotic expression strain. An electrophoretic mobility shift assay of PnbHLH1 protein binding to E-box cis-acting elements verified that PnbHLH1 belonged to the bHLH class transcription factor which could interact with the promoter region of the E-box core sequence. The expression levels of key genes involved in the biosynthesis of triterpenoid saponins in PnbHLH1 transgenic cells were higher than those in the wild cells. Similarly, the total saponin contents were increased in the PnbHLH1 transgenic cell lines compared with the wild cell lines. Such results suggest that the PnbHLH1 transcription factor is a positive regulator in the biosynthesis of triterpenoid saponins in Panax notoginseng. |
format | Online Article Text |
id | pubmed-6152055 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61520552018-11-13 Molecular Cloning and Characterization of PnbHLH1 Transcription Factor in Panax notoginseng Zhang, Xiang Ge, Feng Deng, Bing Shah, Taif Huang, Zhuangjia Liu, Diqiu Chen, Chaoyin Molecules Article Panax notoginseng has been extensively used as a traditional Chinese medicine. In the current study, molecular cloning and characterization of PnbHLH1 transcription factor were explored in Panax notoginseng. The full length of the PnbHLH1 gene obtained by splicing was 1430 bp, encoding 321 amino acids. Prokaryotic expression vector pET-28a-PnbHLH1 was constructed and transferred into the BL21 prokaryotic expression strain. An electrophoretic mobility shift assay of PnbHLH1 protein binding to E-box cis-acting elements verified that PnbHLH1 belonged to the bHLH class transcription factor which could interact with the promoter region of the E-box core sequence. The expression levels of key genes involved in the biosynthesis of triterpenoid saponins in PnbHLH1 transgenic cells were higher than those in the wild cells. Similarly, the total saponin contents were increased in the PnbHLH1 transgenic cell lines compared with the wild cell lines. Such results suggest that the PnbHLH1 transcription factor is a positive regulator in the biosynthesis of triterpenoid saponins in Panax notoginseng. MDPI 2017-07-29 /pmc/articles/PMC6152055/ /pubmed/28758911 http://dx.doi.org/10.3390/molecules22081268 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Xiang Ge, Feng Deng, Bing Shah, Taif Huang, Zhuangjia Liu, Diqiu Chen, Chaoyin Molecular Cloning and Characterization of PnbHLH1 Transcription Factor in Panax notoginseng |
title | Molecular Cloning and Characterization of PnbHLH1 Transcription Factor in Panax notoginseng |
title_full | Molecular Cloning and Characterization of PnbHLH1 Transcription Factor in Panax notoginseng |
title_fullStr | Molecular Cloning and Characterization of PnbHLH1 Transcription Factor in Panax notoginseng |
title_full_unstemmed | Molecular Cloning and Characterization of PnbHLH1 Transcription Factor in Panax notoginseng |
title_short | Molecular Cloning and Characterization of PnbHLH1 Transcription Factor in Panax notoginseng |
title_sort | molecular cloning and characterization of pnbhlh1 transcription factor in panax notoginseng |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152055/ https://www.ncbi.nlm.nih.gov/pubmed/28758911 http://dx.doi.org/10.3390/molecules22081268 |
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