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Capillary-Inserted Rotor Design for HRµMAS NMR-Based Metabolomics on Mass-Limited Neurospheres
Nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical technique and has been widely used in metabolomics. However, the intrinsic low sensitivity of NMR prevents its applications to systems with limited sample availabilities. In this study, a new experimental approach is presented to...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152061/ https://www.ncbi.nlm.nih.gov/pubmed/28771206 http://dx.doi.org/10.3390/molecules22081289 |
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author | Duong, Nghia Tuan Yamato, Masanori Nakano, Masayuki Kume, Satoshi Tamura, Yasuhisa Kataoka, Yosky Wong, Alan Nishiyama, Yusuke |
author_facet | Duong, Nghia Tuan Yamato, Masanori Nakano, Masayuki Kume, Satoshi Tamura, Yasuhisa Kataoka, Yosky Wong, Alan Nishiyama, Yusuke |
author_sort | Duong, Nghia Tuan |
collection | PubMed |
description | Nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical technique and has been widely used in metabolomics. However, the intrinsic low sensitivity of NMR prevents its applications to systems with limited sample availabilities. In this study, a new experimental approach is presented to analyze mass-scarce samples in limited volumes of less than 300 nL with simple handling. The sample is loaded into the glass capillary, and this capillary is then inserted into a Kel-F rotor. The experimental performance of the capillary-inserted rotor (capillary-insert) is investigated on an isotropic solution of sucrose by the use of a high-resolution micro-sized magic angle spinning (HRµMAS) probe. The acquired NMR signal’s sensitivity to a given sample amount is comparable or even higher in comparison to that recorded by the standard solution NMR probe. More importantly, this capillary-insert coupled with the HRµMAS probe allows in-depth studies of heterogeneous samples as the MAS removes the line broadening caused by the heterogeneity. The NMR analyses of mass-limited cultured neurospheres have been demonstrated, resulting in high quality spectra where numerous metabolites are unambiguously identified. |
format | Online Article Text |
id | pubmed-6152061 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61520612018-11-13 Capillary-Inserted Rotor Design for HRµMAS NMR-Based Metabolomics on Mass-Limited Neurospheres Duong, Nghia Tuan Yamato, Masanori Nakano, Masayuki Kume, Satoshi Tamura, Yasuhisa Kataoka, Yosky Wong, Alan Nishiyama, Yusuke Molecules Article Nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical technique and has been widely used in metabolomics. However, the intrinsic low sensitivity of NMR prevents its applications to systems with limited sample availabilities. In this study, a new experimental approach is presented to analyze mass-scarce samples in limited volumes of less than 300 nL with simple handling. The sample is loaded into the glass capillary, and this capillary is then inserted into a Kel-F rotor. The experimental performance of the capillary-inserted rotor (capillary-insert) is investigated on an isotropic solution of sucrose by the use of a high-resolution micro-sized magic angle spinning (HRµMAS) probe. The acquired NMR signal’s sensitivity to a given sample amount is comparable or even higher in comparison to that recorded by the standard solution NMR probe. More importantly, this capillary-insert coupled with the HRµMAS probe allows in-depth studies of heterogeneous samples as the MAS removes the line broadening caused by the heterogeneity. The NMR analyses of mass-limited cultured neurospheres have been demonstrated, resulting in high quality spectra where numerous metabolites are unambiguously identified. MDPI 2017-08-03 /pmc/articles/PMC6152061/ /pubmed/28771206 http://dx.doi.org/10.3390/molecules22081289 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Duong, Nghia Tuan Yamato, Masanori Nakano, Masayuki Kume, Satoshi Tamura, Yasuhisa Kataoka, Yosky Wong, Alan Nishiyama, Yusuke Capillary-Inserted Rotor Design for HRµMAS NMR-Based Metabolomics on Mass-Limited Neurospheres |
title | Capillary-Inserted Rotor Design for HRµMAS NMR-Based Metabolomics on Mass-Limited Neurospheres |
title_full | Capillary-Inserted Rotor Design for HRµMAS NMR-Based Metabolomics on Mass-Limited Neurospheres |
title_fullStr | Capillary-Inserted Rotor Design for HRµMAS NMR-Based Metabolomics on Mass-Limited Neurospheres |
title_full_unstemmed | Capillary-Inserted Rotor Design for HRµMAS NMR-Based Metabolomics on Mass-Limited Neurospheres |
title_short | Capillary-Inserted Rotor Design for HRµMAS NMR-Based Metabolomics on Mass-Limited Neurospheres |
title_sort | capillary-inserted rotor design for hrµmas nmr-based metabolomics on mass-limited neurospheres |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152061/ https://www.ncbi.nlm.nih.gov/pubmed/28771206 http://dx.doi.org/10.3390/molecules22081289 |
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