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Extracts Obtained from Pterocarpus angolensis DC and Ziziphus mucronata Exhibit Antiplasmodial Activity and Inhibit Heat Shock Protein 70 (Hsp70) Function

Malaria parasites are increasingly becoming resistant to currently used antimalarial therapies, therefore there is an urgent need to expand the arsenal of alternative antimalarial drugs. In addition, it is also important to identify novel antimalarial drug targets. In the current study, extracts of...

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Autores principales: Zininga, Tawanda, Anokwuru, Chinedu P., Sigidi, Muendi T., Tshisikhawe, Milingoni P., Ramaite, Isaiah I. D., Traoré, Afsatou N., Hoppe, Heinrich, Shonhai, Addmore, Potgieter, Natasha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152082/
https://www.ncbi.nlm.nih.gov/pubmed/28788073
http://dx.doi.org/10.3390/molecules22081224
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author Zininga, Tawanda
Anokwuru, Chinedu P.
Sigidi, Muendi T.
Tshisikhawe, Milingoni P.
Ramaite, Isaiah I. D.
Traoré, Afsatou N.
Hoppe, Heinrich
Shonhai, Addmore
Potgieter, Natasha
author_facet Zininga, Tawanda
Anokwuru, Chinedu P.
Sigidi, Muendi T.
Tshisikhawe, Milingoni P.
Ramaite, Isaiah I. D.
Traoré, Afsatou N.
Hoppe, Heinrich
Shonhai, Addmore
Potgieter, Natasha
author_sort Zininga, Tawanda
collection PubMed
description Malaria parasites are increasingly becoming resistant to currently used antimalarial therapies, therefore there is an urgent need to expand the arsenal of alternative antimalarial drugs. In addition, it is also important to identify novel antimalarial drug targets. In the current study, extracts of two plants, Pterocarpus angolensis and Ziziphus mucronata were obtained and their antimalarial functions were investigated. Furthermore, we explored the capability of the extracts to inhibit Plasmodium falciparum heat shock protein 70 (Hsp70) function. Heat shock protein 70 (Hsp70) are molecular chaperones whose function is to facilitate protein folding. Plasmodium falciparum the main agent of malaria, expresses two cytosol-localized Hsp70s: PfHsp70-1 and PfHsp70-z. The PfHsp70-z has been reported to be essential for parasite survival, while inhibition of PfHsp70-1 function leads to parasite death. Hence both PfHsp70-1 and PfHsp70-z are potential antimalarial drug targets. Extracts of P. angolensis and Z. mucronata inhibited the basal ATPase and chaperone functions of the two parasite Hsp70s. Furthermore, fractions of P. angolensis and Z. mucronata inhibited P. falciparum 3D7 parasite growth in vitro. The extracts obtained in the current study exhibited antiplasmodial activity as they killed P. falciparum parasites maintained in vitro. In addition, the findings further suggest that some of the compounds in P. angolensis and Z. mucronata may target parasite Hsp70 function.
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spelling pubmed-61520822018-11-13 Extracts Obtained from Pterocarpus angolensis DC and Ziziphus mucronata Exhibit Antiplasmodial Activity and Inhibit Heat Shock Protein 70 (Hsp70) Function Zininga, Tawanda Anokwuru, Chinedu P. Sigidi, Muendi T. Tshisikhawe, Milingoni P. Ramaite, Isaiah I. D. Traoré, Afsatou N. Hoppe, Heinrich Shonhai, Addmore Potgieter, Natasha Molecules Article Malaria parasites are increasingly becoming resistant to currently used antimalarial therapies, therefore there is an urgent need to expand the arsenal of alternative antimalarial drugs. In addition, it is also important to identify novel antimalarial drug targets. In the current study, extracts of two plants, Pterocarpus angolensis and Ziziphus mucronata were obtained and their antimalarial functions were investigated. Furthermore, we explored the capability of the extracts to inhibit Plasmodium falciparum heat shock protein 70 (Hsp70) function. Heat shock protein 70 (Hsp70) are molecular chaperones whose function is to facilitate protein folding. Plasmodium falciparum the main agent of malaria, expresses two cytosol-localized Hsp70s: PfHsp70-1 and PfHsp70-z. The PfHsp70-z has been reported to be essential for parasite survival, while inhibition of PfHsp70-1 function leads to parasite death. Hence both PfHsp70-1 and PfHsp70-z are potential antimalarial drug targets. Extracts of P. angolensis and Z. mucronata inhibited the basal ATPase and chaperone functions of the two parasite Hsp70s. Furthermore, fractions of P. angolensis and Z. mucronata inhibited P. falciparum 3D7 parasite growth in vitro. The extracts obtained in the current study exhibited antiplasmodial activity as they killed P. falciparum parasites maintained in vitro. In addition, the findings further suggest that some of the compounds in P. angolensis and Z. mucronata may target parasite Hsp70 function. MDPI 2017-07-28 /pmc/articles/PMC6152082/ /pubmed/28788073 http://dx.doi.org/10.3390/molecules22081224 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zininga, Tawanda
Anokwuru, Chinedu P.
Sigidi, Muendi T.
Tshisikhawe, Milingoni P.
Ramaite, Isaiah I. D.
Traoré, Afsatou N.
Hoppe, Heinrich
Shonhai, Addmore
Potgieter, Natasha
Extracts Obtained from Pterocarpus angolensis DC and Ziziphus mucronata Exhibit Antiplasmodial Activity and Inhibit Heat Shock Protein 70 (Hsp70) Function
title Extracts Obtained from Pterocarpus angolensis DC and Ziziphus mucronata Exhibit Antiplasmodial Activity and Inhibit Heat Shock Protein 70 (Hsp70) Function
title_full Extracts Obtained from Pterocarpus angolensis DC and Ziziphus mucronata Exhibit Antiplasmodial Activity and Inhibit Heat Shock Protein 70 (Hsp70) Function
title_fullStr Extracts Obtained from Pterocarpus angolensis DC and Ziziphus mucronata Exhibit Antiplasmodial Activity and Inhibit Heat Shock Protein 70 (Hsp70) Function
title_full_unstemmed Extracts Obtained from Pterocarpus angolensis DC and Ziziphus mucronata Exhibit Antiplasmodial Activity and Inhibit Heat Shock Protein 70 (Hsp70) Function
title_short Extracts Obtained from Pterocarpus angolensis DC and Ziziphus mucronata Exhibit Antiplasmodial Activity and Inhibit Heat Shock Protein 70 (Hsp70) Function
title_sort extracts obtained from pterocarpus angolensis dc and ziziphus mucronata exhibit antiplasmodial activity and inhibit heat shock protein 70 (hsp70) function
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152082/
https://www.ncbi.nlm.nih.gov/pubmed/28788073
http://dx.doi.org/10.3390/molecules22081224
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