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Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves
This work describes the isolation and structural elucidation of compounds from the leaves of Myrcia tomentosa (Aubl.) DC. (goiaba-brava) and evaluates the antimicrobial activity of the crude extract, fractions and isolated compounds against bacteria and fungi. Column chromatography was used to fract...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152266/ https://www.ncbi.nlm.nih.gov/pubmed/28677650 http://dx.doi.org/10.3390/molecules22071100 |
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author | da Silva Sa, Fabyola Amaral de Paula, Joelma Abadia Marciano dos Santos, Pierre Alexandre de Almeida Ribeiro Oliveira, Leandra de Almeida Ribeiro Oliveira, Gerlon Liao, Luciano Morais de Paula, Jose Realino do Rosario Rodrigues Silva, Maria |
author_facet | da Silva Sa, Fabyola Amaral de Paula, Joelma Abadia Marciano dos Santos, Pierre Alexandre de Almeida Ribeiro Oliveira, Leandra de Almeida Ribeiro Oliveira, Gerlon Liao, Luciano Morais de Paula, Jose Realino do Rosario Rodrigues Silva, Maria |
author_sort | da Silva Sa, Fabyola Amaral |
collection | PubMed |
description | This work describes the isolation and structural elucidation of compounds from the leaves of Myrcia tomentosa (Aubl.) DC. (goiaba-brava) and evaluates the antimicrobial activity of the crude extract, fractions and isolated compounds against bacteria and fungi. Column chromatography was used to fractionate and purify the extract of the M. tomentosa leaves and the chemical structures of the compounds were determined using spectroscopic techniques. The antibacterial and antifungal activities were assessed using the broth microdilution method. The phytochemical investigation isolated 11 compounds: α-bisabolol, α-bisabolol oxide B, α-cadinol, β-sitosterol, n-pentacosane, n-tetracosane, quercetin, kaempferol, avicularin, juglanin and guaijaverin. The crude ethanolic extract and its fractions were tested against 15 bacteria and 9 yeasts. The crude extract inhibited the in vitro growth of yeasts at concentration of 4 to 32 μg/mL. The hexane, dichloromethane, ethyl acetate and aqueous fractions inhibited Candida sp. at concentrations of 4 to 256 μg/mL, whereas the Cryptococcus sp. isolates were inhibited only by the hexane and dichloromethane fractions in minimal inhibitory concentrations (MICs) at 16 to 64 μg/mL. The flavonoid quercetin-3-O-α-arabinofuranose (avicularin) was the most active compound, inhibiting Candida species in concentrations of 2 to 32 μg/mL. The MIC values suggest potential activity of this plant species against yeast. |
format | Online Article Text |
id | pubmed-6152266 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61522662018-11-13 Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves da Silva Sa, Fabyola Amaral de Paula, Joelma Abadia Marciano dos Santos, Pierre Alexandre de Almeida Ribeiro Oliveira, Leandra de Almeida Ribeiro Oliveira, Gerlon Liao, Luciano Morais de Paula, Jose Realino do Rosario Rodrigues Silva, Maria Molecules Article This work describes the isolation and structural elucidation of compounds from the leaves of Myrcia tomentosa (Aubl.) DC. (goiaba-brava) and evaluates the antimicrobial activity of the crude extract, fractions and isolated compounds against bacteria and fungi. Column chromatography was used to fractionate and purify the extract of the M. tomentosa leaves and the chemical structures of the compounds were determined using spectroscopic techniques. The antibacterial and antifungal activities were assessed using the broth microdilution method. The phytochemical investigation isolated 11 compounds: α-bisabolol, α-bisabolol oxide B, α-cadinol, β-sitosterol, n-pentacosane, n-tetracosane, quercetin, kaempferol, avicularin, juglanin and guaijaverin. The crude ethanolic extract and its fractions were tested against 15 bacteria and 9 yeasts. The crude extract inhibited the in vitro growth of yeasts at concentration of 4 to 32 μg/mL. The hexane, dichloromethane, ethyl acetate and aqueous fractions inhibited Candida sp. at concentrations of 4 to 256 μg/mL, whereas the Cryptococcus sp. isolates were inhibited only by the hexane and dichloromethane fractions in minimal inhibitory concentrations (MICs) at 16 to 64 μg/mL. The flavonoid quercetin-3-O-α-arabinofuranose (avicularin) was the most active compound, inhibiting Candida species in concentrations of 2 to 32 μg/mL. The MIC values suggest potential activity of this plant species against yeast. MDPI 2017-07-04 /pmc/articles/PMC6152266/ /pubmed/28677650 http://dx.doi.org/10.3390/molecules22071100 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article da Silva Sa, Fabyola Amaral de Paula, Joelma Abadia Marciano dos Santos, Pierre Alexandre de Almeida Ribeiro Oliveira, Leandra de Almeida Ribeiro Oliveira, Gerlon Liao, Luciano Morais de Paula, Jose Realino do Rosario Rodrigues Silva, Maria Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves |
title | Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves |
title_full | Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves |
title_fullStr | Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves |
title_full_unstemmed | Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves |
title_short | Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves |
title_sort | phytochemical analysis and antimicrobial activity of myrcia tomentosa (aubl.) dc. leaves |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152266/ https://www.ncbi.nlm.nih.gov/pubmed/28677650 http://dx.doi.org/10.3390/molecules22071100 |
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