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Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves

This work describes the isolation and structural elucidation of compounds from the leaves of Myrcia tomentosa (Aubl.) DC. (goiaba-brava) and evaluates the antimicrobial activity of the crude extract, fractions and isolated compounds against bacteria and fungi. Column chromatography was used to fract...

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Autores principales: da Silva Sa, Fabyola Amaral, de Paula, Joelma Abadia Marciano, dos Santos, Pierre Alexandre, de Almeida Ribeiro Oliveira, Leandra, de Almeida Ribeiro Oliveira, Gerlon, Liao, Luciano Morais, de Paula, Jose Realino, do Rosario Rodrigues Silva, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152266/
https://www.ncbi.nlm.nih.gov/pubmed/28677650
http://dx.doi.org/10.3390/molecules22071100
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author da Silva Sa, Fabyola Amaral
de Paula, Joelma Abadia Marciano
dos Santos, Pierre Alexandre
de Almeida Ribeiro Oliveira, Leandra
de Almeida Ribeiro Oliveira, Gerlon
Liao, Luciano Morais
de Paula, Jose Realino
do Rosario Rodrigues Silva, Maria
author_facet da Silva Sa, Fabyola Amaral
de Paula, Joelma Abadia Marciano
dos Santos, Pierre Alexandre
de Almeida Ribeiro Oliveira, Leandra
de Almeida Ribeiro Oliveira, Gerlon
Liao, Luciano Morais
de Paula, Jose Realino
do Rosario Rodrigues Silva, Maria
author_sort da Silva Sa, Fabyola Amaral
collection PubMed
description This work describes the isolation and structural elucidation of compounds from the leaves of Myrcia tomentosa (Aubl.) DC. (goiaba-brava) and evaluates the antimicrobial activity of the crude extract, fractions and isolated compounds against bacteria and fungi. Column chromatography was used to fractionate and purify the extract of the M. tomentosa leaves and the chemical structures of the compounds were determined using spectroscopic techniques. The antibacterial and antifungal activities were assessed using the broth microdilution method. The phytochemical investigation isolated 11 compounds: α-bisabolol, α-bisabolol oxide B, α-cadinol, β-sitosterol, n-pentacosane, n-tetracosane, quercetin, kaempferol, avicularin, juglanin and guaijaverin. The crude ethanolic extract and its fractions were tested against 15 bacteria and 9 yeasts. The crude extract inhibited the in vitro growth of yeasts at concentration of 4 to 32 μg/mL. The hexane, dichloromethane, ethyl acetate and aqueous fractions inhibited Candida sp. at concentrations of 4 to 256 μg/mL, whereas the Cryptococcus sp. isolates were inhibited only by the hexane and dichloromethane fractions in minimal inhibitory concentrations (MICs) at 16 to 64 μg/mL. The flavonoid quercetin-3-O-α-arabinofuranose (avicularin) was the most active compound, inhibiting Candida species in concentrations of 2 to 32 μg/mL. The MIC values suggest potential activity of this plant species against yeast.
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spelling pubmed-61522662018-11-13 Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves da Silva Sa, Fabyola Amaral de Paula, Joelma Abadia Marciano dos Santos, Pierre Alexandre de Almeida Ribeiro Oliveira, Leandra de Almeida Ribeiro Oliveira, Gerlon Liao, Luciano Morais de Paula, Jose Realino do Rosario Rodrigues Silva, Maria Molecules Article This work describes the isolation and structural elucidation of compounds from the leaves of Myrcia tomentosa (Aubl.) DC. (goiaba-brava) and evaluates the antimicrobial activity of the crude extract, fractions and isolated compounds against bacteria and fungi. Column chromatography was used to fractionate and purify the extract of the M. tomentosa leaves and the chemical structures of the compounds were determined using spectroscopic techniques. The antibacterial and antifungal activities were assessed using the broth microdilution method. The phytochemical investigation isolated 11 compounds: α-bisabolol, α-bisabolol oxide B, α-cadinol, β-sitosterol, n-pentacosane, n-tetracosane, quercetin, kaempferol, avicularin, juglanin and guaijaverin. The crude ethanolic extract and its fractions were tested against 15 bacteria and 9 yeasts. The crude extract inhibited the in vitro growth of yeasts at concentration of 4 to 32 μg/mL. The hexane, dichloromethane, ethyl acetate and aqueous fractions inhibited Candida sp. at concentrations of 4 to 256 μg/mL, whereas the Cryptococcus sp. isolates were inhibited only by the hexane and dichloromethane fractions in minimal inhibitory concentrations (MICs) at 16 to 64 μg/mL. The flavonoid quercetin-3-O-α-arabinofuranose (avicularin) was the most active compound, inhibiting Candida species in concentrations of 2 to 32 μg/mL. The MIC values suggest potential activity of this plant species against yeast. MDPI 2017-07-04 /pmc/articles/PMC6152266/ /pubmed/28677650 http://dx.doi.org/10.3390/molecules22071100 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
da Silva Sa, Fabyola Amaral
de Paula, Joelma Abadia Marciano
dos Santos, Pierre Alexandre
de Almeida Ribeiro Oliveira, Leandra
de Almeida Ribeiro Oliveira, Gerlon
Liao, Luciano Morais
de Paula, Jose Realino
do Rosario Rodrigues Silva, Maria
Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves
title Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves
title_full Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves
title_fullStr Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves
title_full_unstemmed Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves
title_short Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves
title_sort phytochemical analysis and antimicrobial activity of myrcia tomentosa (aubl.) dc. leaves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152266/
https://www.ncbi.nlm.nih.gov/pubmed/28677650
http://dx.doi.org/10.3390/molecules22071100
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