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A Pectic Polysaccharide from Sijunzi Decoction Promotes the Antioxidant Defenses of SW480 Cells

Sijunzi Decoction (SJZD) is a formula used for the treatment of spleen deficiency and gastrointestinal diseases in Traditional Chinese Medicine. Polysaccharides are reported to be the main components of SJZD responsible for its bio-functions. However, highly purified and clearly characterized polysa...

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Autores principales: Huang, Chao, Zhu, Zhongkai, Cao, Xiyue, Chen, Xingfu, Fu, Yuping, Chen, Zhengli, Li, Lixia, Song, Xu, Jia, Renyong, Yin, Zhongqiong, Ye, Gang, Feng, Bin, Zou, Yuanfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152311/
https://www.ncbi.nlm.nih.gov/pubmed/28805701
http://dx.doi.org/10.3390/molecules22081341
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author Huang, Chao
Zhu, Zhongkai
Cao, Xiyue
Chen, Xingfu
Fu, Yuping
Chen, Zhengli
Li, Lixia
Song, Xu
Jia, Renyong
Yin, Zhongqiong
Ye, Gang
Feng, Bin
Zou, Yuanfeng
author_facet Huang, Chao
Zhu, Zhongkai
Cao, Xiyue
Chen, Xingfu
Fu, Yuping
Chen, Zhengli
Li, Lixia
Song, Xu
Jia, Renyong
Yin, Zhongqiong
Ye, Gang
Feng, Bin
Zou, Yuanfeng
author_sort Huang, Chao
collection PubMed
description Sijunzi Decoction (SJZD) is a formula used for the treatment of spleen deficiency and gastrointestinal diseases in Traditional Chinese Medicine. Polysaccharides are reported to be the main components of SJZD responsible for its bio-functions. However, highly purified and clearly characterized polysaccharides from SJZD are not well described. Here we obtained a purified polysaccharide (SJZDP-II-I) from SJZD using ion exchange chromatography and gel filtration. Structure analysis by FT-IR and NMR identified SJZDP-II-I as a typical pectic polysaccharide with homogalacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and II as side chains. In vitro studies indicated that SJZDP-II-I treatment could significantly enhance the total antioxidant capacity of SW480 cells, resulting from the promoted expressions of antioxidant enzymes and their master regulator PGC-1α, which would be valuable for further research and applications.
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spelling pubmed-61523112018-11-13 A Pectic Polysaccharide from Sijunzi Decoction Promotes the Antioxidant Defenses of SW480 Cells Huang, Chao Zhu, Zhongkai Cao, Xiyue Chen, Xingfu Fu, Yuping Chen, Zhengli Li, Lixia Song, Xu Jia, Renyong Yin, Zhongqiong Ye, Gang Feng, Bin Zou, Yuanfeng Molecules Article Sijunzi Decoction (SJZD) is a formula used for the treatment of spleen deficiency and gastrointestinal diseases in Traditional Chinese Medicine. Polysaccharides are reported to be the main components of SJZD responsible for its bio-functions. However, highly purified and clearly characterized polysaccharides from SJZD are not well described. Here we obtained a purified polysaccharide (SJZDP-II-I) from SJZD using ion exchange chromatography and gel filtration. Structure analysis by FT-IR and NMR identified SJZDP-II-I as a typical pectic polysaccharide with homogalacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and II as side chains. In vitro studies indicated that SJZDP-II-I treatment could significantly enhance the total antioxidant capacity of SW480 cells, resulting from the promoted expressions of antioxidant enzymes and their master regulator PGC-1α, which would be valuable for further research and applications. MDPI 2017-08-12 /pmc/articles/PMC6152311/ /pubmed/28805701 http://dx.doi.org/10.3390/molecules22081341 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huang, Chao
Zhu, Zhongkai
Cao, Xiyue
Chen, Xingfu
Fu, Yuping
Chen, Zhengli
Li, Lixia
Song, Xu
Jia, Renyong
Yin, Zhongqiong
Ye, Gang
Feng, Bin
Zou, Yuanfeng
A Pectic Polysaccharide from Sijunzi Decoction Promotes the Antioxidant Defenses of SW480 Cells
title A Pectic Polysaccharide from Sijunzi Decoction Promotes the Antioxidant Defenses of SW480 Cells
title_full A Pectic Polysaccharide from Sijunzi Decoction Promotes the Antioxidant Defenses of SW480 Cells
title_fullStr A Pectic Polysaccharide from Sijunzi Decoction Promotes the Antioxidant Defenses of SW480 Cells
title_full_unstemmed A Pectic Polysaccharide from Sijunzi Decoction Promotes the Antioxidant Defenses of SW480 Cells
title_short A Pectic Polysaccharide from Sijunzi Decoction Promotes the Antioxidant Defenses of SW480 Cells
title_sort pectic polysaccharide from sijunzi decoction promotes the antioxidant defenses of sw480 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152311/
https://www.ncbi.nlm.nih.gov/pubmed/28805701
http://dx.doi.org/10.3390/molecules22081341
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