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A Purified Serine Protease from Nereis virens and Its Impaction of Apoptosis on Human Lung Cancer Cells

Nereis active protease (NAP) is a novel fibrinolytic active serine protease from the polychaete Nereis virens. In this study, NAP was purified from Nereis virens and the effects of NAP on human lung cancer cells were investigated. Our results indicated that NAP inhibited the proliferation and induce...

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Autores principales: Tang, Yunping, Yu, Fangmiao, Zhang, Guomei, Yang, Zuisu, Huang, Fangfang, Ding, Guofang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152330/
https://www.ncbi.nlm.nih.gov/pubmed/28686182
http://dx.doi.org/10.3390/molecules22071123
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author Tang, Yunping
Yu, Fangmiao
Zhang, Guomei
Yang, Zuisu
Huang, Fangfang
Ding, Guofang
author_facet Tang, Yunping
Yu, Fangmiao
Zhang, Guomei
Yang, Zuisu
Huang, Fangfang
Ding, Guofang
author_sort Tang, Yunping
collection PubMed
description Nereis active protease (NAP) is a novel fibrinolytic active serine protease from the polychaete Nereis virens. In this study, NAP was purified from Nereis virens and the effects of NAP on human lung cancer cells were investigated. Our results indicated that NAP inhibited the proliferation and induced apoptosis of H1299 cells in a time- and dose-dependent manner. The loss of mitochondrial membrane potential, the activation of Bax and cleaved-caspase 3/9, the release of cytochrome C, and the suppression of Bcl-2 and poly-ADP ribose polymerase were observed in NAP-treated H1299 cells by flow cytometry and Western blotting. Moreover, the expression levels of Bax and Bcl-2 mRNA were determined by real-time quantitative polymerase chain reaction and the Bax/Bcl-2 expression ratio was increased in the NAP-treated cell lines. The results indicated that NAP-induced apoptosis may be related to mitochondria mediated apoptosis and occurs through caspase-dependent pathways. Then, the effects of NAP on tumor growth in animal models were observed, where 5 or 10 mg/kg of NAP noticeably reduced tumor volume and weight and increased apoptosis as determined by Western blotting when compared to the negative control group. Therefore, our findings suggest that NAP could be a hopeful anticancer medicine for its propensity to inhibit growth and induce of apoptosis in human lung cancer cells.
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spelling pubmed-61523302018-11-13 A Purified Serine Protease from Nereis virens and Its Impaction of Apoptosis on Human Lung Cancer Cells Tang, Yunping Yu, Fangmiao Zhang, Guomei Yang, Zuisu Huang, Fangfang Ding, Guofang Molecules Article Nereis active protease (NAP) is a novel fibrinolytic active serine protease from the polychaete Nereis virens. In this study, NAP was purified from Nereis virens and the effects of NAP on human lung cancer cells were investigated. Our results indicated that NAP inhibited the proliferation and induced apoptosis of H1299 cells in a time- and dose-dependent manner. The loss of mitochondrial membrane potential, the activation of Bax and cleaved-caspase 3/9, the release of cytochrome C, and the suppression of Bcl-2 and poly-ADP ribose polymerase were observed in NAP-treated H1299 cells by flow cytometry and Western blotting. Moreover, the expression levels of Bax and Bcl-2 mRNA were determined by real-time quantitative polymerase chain reaction and the Bax/Bcl-2 expression ratio was increased in the NAP-treated cell lines. The results indicated that NAP-induced apoptosis may be related to mitochondria mediated apoptosis and occurs through caspase-dependent pathways. Then, the effects of NAP on tumor growth in animal models were observed, where 5 or 10 mg/kg of NAP noticeably reduced tumor volume and weight and increased apoptosis as determined by Western blotting when compared to the negative control group. Therefore, our findings suggest that NAP could be a hopeful anticancer medicine for its propensity to inhibit growth and induce of apoptosis in human lung cancer cells. MDPI 2017-07-07 /pmc/articles/PMC6152330/ /pubmed/28686182 http://dx.doi.org/10.3390/molecules22071123 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tang, Yunping
Yu, Fangmiao
Zhang, Guomei
Yang, Zuisu
Huang, Fangfang
Ding, Guofang
A Purified Serine Protease from Nereis virens and Its Impaction of Apoptosis on Human Lung Cancer Cells
title A Purified Serine Protease from Nereis virens and Its Impaction of Apoptosis on Human Lung Cancer Cells
title_full A Purified Serine Protease from Nereis virens and Its Impaction of Apoptosis on Human Lung Cancer Cells
title_fullStr A Purified Serine Protease from Nereis virens and Its Impaction of Apoptosis on Human Lung Cancer Cells
title_full_unstemmed A Purified Serine Protease from Nereis virens and Its Impaction of Apoptosis on Human Lung Cancer Cells
title_short A Purified Serine Protease from Nereis virens and Its Impaction of Apoptosis on Human Lung Cancer Cells
title_sort purified serine protease from nereis virens and its impaction of apoptosis on human lung cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152330/
https://www.ncbi.nlm.nih.gov/pubmed/28686182
http://dx.doi.org/10.3390/molecules22071123
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