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Baccharis trimera (Less.) DC Exhibits an Anti-Adipogenic Effect by Inhibiting the Expression of Proteins Involved in Adipocyte Differentiation

Baccharis trimera (Less.) DC (gorse) is a plant popularly used for the treatment of obesity. In this study, we prepared three B. trimera extracts aqueous extract (AE), decoction (AE-D), and methanol extract (ME) and investigated their antioxidant effects in six different tests and their anti-adipoge...

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Autores principales: de Souza Marinho do Nascimento, Daniele, Oliveira, Ruth Medeiros, Camara, Rafael Barros Gomes, Gomes, Dayanne Lopes, Monte, Jessika Fernanda Santiago, Costa, Mariana Santana Santos Pereira, Fernandes, Júlia Moraes, Langassner, Silvana Maria Zucolotto, Rocha, Hugo Alexandre Oliveira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152623/
https://www.ncbi.nlm.nih.gov/pubmed/28604636
http://dx.doi.org/10.3390/molecules22060972
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author de Souza Marinho do Nascimento, Daniele
Oliveira, Ruth Medeiros
Camara, Rafael Barros Gomes
Gomes, Dayanne Lopes
Monte, Jessika Fernanda Santiago
Costa, Mariana Santana Santos Pereira
Fernandes, Júlia Moraes
Langassner, Silvana Maria Zucolotto
Rocha, Hugo Alexandre Oliveira
author_facet de Souza Marinho do Nascimento, Daniele
Oliveira, Ruth Medeiros
Camara, Rafael Barros Gomes
Gomes, Dayanne Lopes
Monte, Jessika Fernanda Santiago
Costa, Mariana Santana Santos Pereira
Fernandes, Júlia Moraes
Langassner, Silvana Maria Zucolotto
Rocha, Hugo Alexandre Oliveira
author_sort de Souza Marinho do Nascimento, Daniele
collection PubMed
description Baccharis trimera (Less.) DC (gorse) is a plant popularly used for the treatment of obesity. In this study, we prepared three B. trimera extracts aqueous extract (AE), decoction (AE-D), and methanol extract (ME) and investigated their antioxidant effects in six different tests and their anti-adipogenic effect in 3T3-L1 cells. The extracts showed a dose-dependent antioxidant activity in all tests. AE was the most potent antioxidant in copper and ferric ion chelation assays, whereas AE-D was the most potent in superoxide and hydroxyl radical scavenging assays, reducing power assay, and total antioxidant capacity analysis. Only ME showed a cytotoxic effect against 3T3-L1 cells. Lipid accumulation decreased in 3T3-L1 adipocytes in the presence of AE and AE-D extracts (0.5 to 1.0 mg/mL). In addition, the extracts dramatically attenuated the levels of adipogenic transcriptional factors, including CCAAT enhancer-binding protein α (C/EBPα), CCAAT enhancer-binding protein β (C/EBPβ), and gamma receptors by peroxisome proliferators (PPARγ), during adipogenesis. AE-D (1.0 mg/mL) caused an approximately 90% reduction in the levels of these molecules. We propose that B. trimera has an anti-adipogenic effect and could be used in the development of functional foods.
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spelling pubmed-61526232018-11-13 Baccharis trimera (Less.) DC Exhibits an Anti-Adipogenic Effect by Inhibiting the Expression of Proteins Involved in Adipocyte Differentiation de Souza Marinho do Nascimento, Daniele Oliveira, Ruth Medeiros Camara, Rafael Barros Gomes Gomes, Dayanne Lopes Monte, Jessika Fernanda Santiago Costa, Mariana Santana Santos Pereira Fernandes, Júlia Moraes Langassner, Silvana Maria Zucolotto Rocha, Hugo Alexandre Oliveira Molecules Article Baccharis trimera (Less.) DC (gorse) is a plant popularly used for the treatment of obesity. In this study, we prepared three B. trimera extracts aqueous extract (AE), decoction (AE-D), and methanol extract (ME) and investigated their antioxidant effects in six different tests and their anti-adipogenic effect in 3T3-L1 cells. The extracts showed a dose-dependent antioxidant activity in all tests. AE was the most potent antioxidant in copper and ferric ion chelation assays, whereas AE-D was the most potent in superoxide and hydroxyl radical scavenging assays, reducing power assay, and total antioxidant capacity analysis. Only ME showed a cytotoxic effect against 3T3-L1 cells. Lipid accumulation decreased in 3T3-L1 adipocytes in the presence of AE and AE-D extracts (0.5 to 1.0 mg/mL). In addition, the extracts dramatically attenuated the levels of adipogenic transcriptional factors, including CCAAT enhancer-binding protein α (C/EBPα), CCAAT enhancer-binding protein β (C/EBPβ), and gamma receptors by peroxisome proliferators (PPARγ), during adipogenesis. AE-D (1.0 mg/mL) caused an approximately 90% reduction in the levels of these molecules. We propose that B. trimera has an anti-adipogenic effect and could be used in the development of functional foods. MDPI 2017-06-12 /pmc/articles/PMC6152623/ /pubmed/28604636 http://dx.doi.org/10.3390/molecules22060972 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Souza Marinho do Nascimento, Daniele
Oliveira, Ruth Medeiros
Camara, Rafael Barros Gomes
Gomes, Dayanne Lopes
Monte, Jessika Fernanda Santiago
Costa, Mariana Santana Santos Pereira
Fernandes, Júlia Moraes
Langassner, Silvana Maria Zucolotto
Rocha, Hugo Alexandre Oliveira
Baccharis trimera (Less.) DC Exhibits an Anti-Adipogenic Effect by Inhibiting the Expression of Proteins Involved in Adipocyte Differentiation
title Baccharis trimera (Less.) DC Exhibits an Anti-Adipogenic Effect by Inhibiting the Expression of Proteins Involved in Adipocyte Differentiation
title_full Baccharis trimera (Less.) DC Exhibits an Anti-Adipogenic Effect by Inhibiting the Expression of Proteins Involved in Adipocyte Differentiation
title_fullStr Baccharis trimera (Less.) DC Exhibits an Anti-Adipogenic Effect by Inhibiting the Expression of Proteins Involved in Adipocyte Differentiation
title_full_unstemmed Baccharis trimera (Less.) DC Exhibits an Anti-Adipogenic Effect by Inhibiting the Expression of Proteins Involved in Adipocyte Differentiation
title_short Baccharis trimera (Less.) DC Exhibits an Anti-Adipogenic Effect by Inhibiting the Expression of Proteins Involved in Adipocyte Differentiation
title_sort baccharis trimera (less.) dc exhibits an anti-adipogenic effect by inhibiting the expression of proteins involved in adipocyte differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152623/
https://www.ncbi.nlm.nih.gov/pubmed/28604636
http://dx.doi.org/10.3390/molecules22060972
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