Cargando…
Characterization of Metabolite Profile in Phyllanthus niruri and Correlation with Bioactivity Elucidated by Nuclear Magnetic Resonance Based Metabolomics
Phyllanthus niruri is an important medicinal plant. To standardize the extract and guarantee its maximum benefit, processing methods optimization ought to be amenable and beneficial. Herein, three dried P. niruri samples, air (AD), freeze (FD) and oven (OD), extracted with various ethanol to water r...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152626/ https://www.ncbi.nlm.nih.gov/pubmed/28556789 http://dx.doi.org/10.3390/molecules22060902 |
_version_ | 1783357398024454144 |
---|---|
author | Mediani, Ahmed Abas, Faridah Maulidiani, M. Khatib, Alfi Tan, Chin Ping Safinar Ismail, Intan Shaari, Khozirah Ismail, Amin |
author_facet | Mediani, Ahmed Abas, Faridah Maulidiani, M. Khatib, Alfi Tan, Chin Ping Safinar Ismail, Intan Shaari, Khozirah Ismail, Amin |
author_sort | Mediani, Ahmed |
collection | PubMed |
description | Phyllanthus niruri is an important medicinal plant. To standardize the extract and guarantee its maximum benefit, processing methods optimization ought to be amenable and beneficial. Herein, three dried P. niruri samples, air (AD), freeze (FD) and oven (OD), extracted with various ethanol to water ratios (0%, 50%, 70%, 80% and 100%) were evaluated for their metabolite changes using proton nuclear magnetic resonance ((1)H-NMR)-based metabolomics approach. The amino acids analysis showed that FD P. niruri exhibited higher content of most amino acids compared to the other dried samples. Based on principal component analysis (PCA), the FD P. niruri extracted with 80% ethanol contained higher amounts of hypophyllanthin and phenolic compounds based on the loading plot. The partial least-square (PLS) results showed that the phytochemicals, including hypophyllanthin, catechin, epicatechin, rutin, quercetin and chlorogenic, caffeic, malic and gallic acids were correlated with antioxidant and α-glucosidase inhibitory activities, which were higher in the FD material extracted with 80% ethanol. This report optimized the effect of drying and ethanol ratios and these findings demonstrate that NMR-based metabolomics was an applicable approach. The FD P. niruri extracted with 80% ethanol can be used as afunctional food ingredient for nutraceutical or in medicinal preparation. |
format | Online Article Text |
id | pubmed-6152626 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61526262018-11-13 Characterization of Metabolite Profile in Phyllanthus niruri and Correlation with Bioactivity Elucidated by Nuclear Magnetic Resonance Based Metabolomics Mediani, Ahmed Abas, Faridah Maulidiani, M. Khatib, Alfi Tan, Chin Ping Safinar Ismail, Intan Shaari, Khozirah Ismail, Amin Molecules Article Phyllanthus niruri is an important medicinal plant. To standardize the extract and guarantee its maximum benefit, processing methods optimization ought to be amenable and beneficial. Herein, three dried P. niruri samples, air (AD), freeze (FD) and oven (OD), extracted with various ethanol to water ratios (0%, 50%, 70%, 80% and 100%) were evaluated for their metabolite changes using proton nuclear magnetic resonance ((1)H-NMR)-based metabolomics approach. The amino acids analysis showed that FD P. niruri exhibited higher content of most amino acids compared to the other dried samples. Based on principal component analysis (PCA), the FD P. niruri extracted with 80% ethanol contained higher amounts of hypophyllanthin and phenolic compounds based on the loading plot. The partial least-square (PLS) results showed that the phytochemicals, including hypophyllanthin, catechin, epicatechin, rutin, quercetin and chlorogenic, caffeic, malic and gallic acids were correlated with antioxidant and α-glucosidase inhibitory activities, which were higher in the FD material extracted with 80% ethanol. This report optimized the effect of drying and ethanol ratios and these findings demonstrate that NMR-based metabolomics was an applicable approach. The FD P. niruri extracted with 80% ethanol can be used as afunctional food ingredient for nutraceutical or in medicinal preparation. MDPI 2017-05-30 /pmc/articles/PMC6152626/ /pubmed/28556789 http://dx.doi.org/10.3390/molecules22060902 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mediani, Ahmed Abas, Faridah Maulidiani, M. Khatib, Alfi Tan, Chin Ping Safinar Ismail, Intan Shaari, Khozirah Ismail, Amin Characterization of Metabolite Profile in Phyllanthus niruri and Correlation with Bioactivity Elucidated by Nuclear Magnetic Resonance Based Metabolomics |
title | Characterization of Metabolite Profile in Phyllanthus niruri and Correlation with Bioactivity Elucidated by Nuclear Magnetic Resonance Based Metabolomics |
title_full | Characterization of Metabolite Profile in Phyllanthus niruri and Correlation with Bioactivity Elucidated by Nuclear Magnetic Resonance Based Metabolomics |
title_fullStr | Characterization of Metabolite Profile in Phyllanthus niruri and Correlation with Bioactivity Elucidated by Nuclear Magnetic Resonance Based Metabolomics |
title_full_unstemmed | Characterization of Metabolite Profile in Phyllanthus niruri and Correlation with Bioactivity Elucidated by Nuclear Magnetic Resonance Based Metabolomics |
title_short | Characterization of Metabolite Profile in Phyllanthus niruri and Correlation with Bioactivity Elucidated by Nuclear Magnetic Resonance Based Metabolomics |
title_sort | characterization of metabolite profile in phyllanthus niruri and correlation with bioactivity elucidated by nuclear magnetic resonance based metabolomics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152626/ https://www.ncbi.nlm.nih.gov/pubmed/28556789 http://dx.doi.org/10.3390/molecules22060902 |
work_keys_str_mv | AT medianiahmed characterizationofmetaboliteprofileinphyllanthusniruriandcorrelationwithbioactivityelucidatedbynuclearmagneticresonancebasedmetabolomics AT abasfaridah characterizationofmetaboliteprofileinphyllanthusniruriandcorrelationwithbioactivityelucidatedbynuclearmagneticresonancebasedmetabolomics AT maulidianim characterizationofmetaboliteprofileinphyllanthusniruriandcorrelationwithbioactivityelucidatedbynuclearmagneticresonancebasedmetabolomics AT khatibalfi characterizationofmetaboliteprofileinphyllanthusniruriandcorrelationwithbioactivityelucidatedbynuclearmagneticresonancebasedmetabolomics AT tanchinping characterizationofmetaboliteprofileinphyllanthusniruriandcorrelationwithbioactivityelucidatedbynuclearmagneticresonancebasedmetabolomics AT safinarismailintan characterizationofmetaboliteprofileinphyllanthusniruriandcorrelationwithbioactivityelucidatedbynuclearmagneticresonancebasedmetabolomics AT shaarikhozirah characterizationofmetaboliteprofileinphyllanthusniruriandcorrelationwithbioactivityelucidatedbynuclearmagneticresonancebasedmetabolomics AT ismailamin characterizationofmetaboliteprofileinphyllanthusniruriandcorrelationwithbioactivityelucidatedbynuclearmagneticresonancebasedmetabolomics |