Cargando…

Hochu-ekki-to Treatment Improves Reproductive and Immune Modulation in the Stress-Induced Rat Model of Polycystic Ovarian Syndrome

The traditional herbal medicine, Hochu-ekki-to, has been shown to have preventive effects on viral infection and stress. This study aimed to evaluate the clinical effects of Hochu-ekki-to on two stress-related rat models of polycystic ovarian syndrome. Female Sprague-Dawley rats were divided into co...

Descripción completa

Detalles Bibliográficos
Autores principales: Park, Eunkuk, Choi, Chun Whan, Kim, Soo Jeong, Kim, Yong-In, Sin, Samkee, Chu, Jong-Phil, Heo, Jun Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152635/
https://www.ncbi.nlm.nih.gov/pubmed/28608837
http://dx.doi.org/10.3390/molecules22060978
_version_ 1783357400224366592
author Park, Eunkuk
Choi, Chun Whan
Kim, Soo Jeong
Kim, Yong-In
Sin, Samkee
Chu, Jong-Phil
Heo, Jun Young
author_facet Park, Eunkuk
Choi, Chun Whan
Kim, Soo Jeong
Kim, Yong-In
Sin, Samkee
Chu, Jong-Phil
Heo, Jun Young
author_sort Park, Eunkuk
collection PubMed
description The traditional herbal medicine, Hochu-ekki-to, has been shown to have preventive effects on viral infection and stress. This study aimed to evaluate the clinical effects of Hochu-ekki-to on two stress-related rat models of polycystic ovarian syndrome. Female Sprague-Dawley rats were divided into control and treatment groups, the latter of which were subjected to stress induced by exposure to adrenocorticotropic hormone (ACTH) or cold temperatures. After these stress inductions, rats were orally treated with dissolved Hochu-ekki-to once per day for 7 days. Rats subjected to the two different stressors exhibited upregulation of steroid hormone receptors (in ovaries) and reproductive hormones (in blood), and consequent stimulation of abnormal follicle development accompanied by elevation of Hsp 90 expression (in ovaries). Treatment with Hochu-ekki-to for 7 days after stress induction increased immune functions, reduced the stress-induced activation of Hsp 90, and normalized the levels of the tested steroid hormone receptors and reproductive hormones. Our findings suggest that stress stimulations may promote the activation of Hsp 90 via the dysregulation of steroid hormone receptors and reproductive hormones, but that post-stress treatment with Hochu-ekki-to improves reproductive and immune functions in the ovaries of stressed rats.
format Online
Article
Text
id pubmed-6152635
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-61526352018-11-13 Hochu-ekki-to Treatment Improves Reproductive and Immune Modulation in the Stress-Induced Rat Model of Polycystic Ovarian Syndrome Park, Eunkuk Choi, Chun Whan Kim, Soo Jeong Kim, Yong-In Sin, Samkee Chu, Jong-Phil Heo, Jun Young Molecules Article The traditional herbal medicine, Hochu-ekki-to, has been shown to have preventive effects on viral infection and stress. This study aimed to evaluate the clinical effects of Hochu-ekki-to on two stress-related rat models of polycystic ovarian syndrome. Female Sprague-Dawley rats were divided into control and treatment groups, the latter of which were subjected to stress induced by exposure to adrenocorticotropic hormone (ACTH) or cold temperatures. After these stress inductions, rats were orally treated with dissolved Hochu-ekki-to once per day for 7 days. Rats subjected to the two different stressors exhibited upregulation of steroid hormone receptors (in ovaries) and reproductive hormones (in blood), and consequent stimulation of abnormal follicle development accompanied by elevation of Hsp 90 expression (in ovaries). Treatment with Hochu-ekki-to for 7 days after stress induction increased immune functions, reduced the stress-induced activation of Hsp 90, and normalized the levels of the tested steroid hormone receptors and reproductive hormones. Our findings suggest that stress stimulations may promote the activation of Hsp 90 via the dysregulation of steroid hormone receptors and reproductive hormones, but that post-stress treatment with Hochu-ekki-to improves reproductive and immune functions in the ovaries of stressed rats. MDPI 2017-06-13 /pmc/articles/PMC6152635/ /pubmed/28608837 http://dx.doi.org/10.3390/molecules22060978 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Eunkuk
Choi, Chun Whan
Kim, Soo Jeong
Kim, Yong-In
Sin, Samkee
Chu, Jong-Phil
Heo, Jun Young
Hochu-ekki-to Treatment Improves Reproductive and Immune Modulation in the Stress-Induced Rat Model of Polycystic Ovarian Syndrome
title Hochu-ekki-to Treatment Improves Reproductive and Immune Modulation in the Stress-Induced Rat Model of Polycystic Ovarian Syndrome
title_full Hochu-ekki-to Treatment Improves Reproductive and Immune Modulation in the Stress-Induced Rat Model of Polycystic Ovarian Syndrome
title_fullStr Hochu-ekki-to Treatment Improves Reproductive and Immune Modulation in the Stress-Induced Rat Model of Polycystic Ovarian Syndrome
title_full_unstemmed Hochu-ekki-to Treatment Improves Reproductive and Immune Modulation in the Stress-Induced Rat Model of Polycystic Ovarian Syndrome
title_short Hochu-ekki-to Treatment Improves Reproductive and Immune Modulation in the Stress-Induced Rat Model of Polycystic Ovarian Syndrome
title_sort hochu-ekki-to treatment improves reproductive and immune modulation in the stress-induced rat model of polycystic ovarian syndrome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152635/
https://www.ncbi.nlm.nih.gov/pubmed/28608837
http://dx.doi.org/10.3390/molecules22060978
work_keys_str_mv AT parkeunkuk hochuekkitotreatmentimprovesreproductiveandimmunemodulationinthestressinducedratmodelofpolycysticovariansyndrome
AT choichunwhan hochuekkitotreatmentimprovesreproductiveandimmunemodulationinthestressinducedratmodelofpolycysticovariansyndrome
AT kimsoojeong hochuekkitotreatmentimprovesreproductiveandimmunemodulationinthestressinducedratmodelofpolycysticovariansyndrome
AT kimyongin hochuekkitotreatmentimprovesreproductiveandimmunemodulationinthestressinducedratmodelofpolycysticovariansyndrome
AT sinsamkee hochuekkitotreatmentimprovesreproductiveandimmunemodulationinthestressinducedratmodelofpolycysticovariansyndrome
AT chujongphil hochuekkitotreatmentimprovesreproductiveandimmunemodulationinthestressinducedratmodelofpolycysticovariansyndrome
AT heojunyoung hochuekkitotreatmentimprovesreproductiveandimmunemodulationinthestressinducedratmodelofpolycysticovariansyndrome