Cargando…

Anti-Inflammatory Activities and Liver Protection of Alisol F and 25-Anhydroalisol F through the Inhibition of MAPK, STAT3, and NF-κB Activation In Vitro and In Vivo

Alisol F and 25-anhydroalisol F isolated from Alisma orientale, were proved to exhibit anti-inflammatory potential in our previous work. In the current study, the anti-inflammatory effects and action mechanisms of alisol F and 25-anhydroalisol F were investigated in vitro. Moreover, the pharmacologi...

Descripción completa

Detalles Bibliográficos
Autores principales: Bi, Xiaoxu, Wang, Pu, Ma, Qingjuan, Han, Li, Wang, Xingbo, Mu, Yu, Guan, Peipei, Qu, Xiaodan, Wang, Zhanyou, Huang, Xueshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152757/
https://www.ncbi.nlm.nih.gov/pubmed/28594379
http://dx.doi.org/10.3390/molecules22060951
_version_ 1783357426277285888
author Bi, Xiaoxu
Wang, Pu
Ma, Qingjuan
Han, Li
Wang, Xingbo
Mu, Yu
Guan, Peipei
Qu, Xiaodan
Wang, Zhanyou
Huang, Xueshi
author_facet Bi, Xiaoxu
Wang, Pu
Ma, Qingjuan
Han, Li
Wang, Xingbo
Mu, Yu
Guan, Peipei
Qu, Xiaodan
Wang, Zhanyou
Huang, Xueshi
author_sort Bi, Xiaoxu
collection PubMed
description Alisol F and 25-anhydroalisol F isolated from Alisma orientale, were proved to exhibit anti-inflammatory potential in our previous work. In the current study, the anti-inflammatory effects and action mechanisms of alisol F and 25-anhydroalisol F were investigated in vitro. Moreover, the pharmacological effects of alisol F in lipopolysaccharide (LPS)/d-galactosamine (d-gal)-induced acute liver-injured mice were evaluated. The results demonstrated that alisol F and 25-anhydroalisol F could suppress LPS-induced production of nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), and interleukin-1β (IL-1β), as well as inhibit the mRNA and protein levels of inducible nitric oxide (iNOS) and cyclooxygenase-2 (COX-2). In addition, we investigated the role of alisol F and 25-anhydroalisol F in mediating mitogen-activated protein kinases (MAPKs), signal transducers, and activators of transcription 3 (STAT3) and nuclear factor κB (NF-κB) pathways involved in the inflammation process of LPS-stimulated RAW 264.7 cells. The phosphorylation of ERK, JNK, p38, and STAT3, and the NF-κB signaling pathway, were obviously suppressed in alisol F and 25-anhydroalisol F treated cells. Results obtained from in vitro experiments suggested alisol F obviously improved liver pathological injury by inhibiting the production of TNF-α, IL-1β, and IL-6, and significantly decreasing the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in LPS/d-gal-induced mice. Furthermore, the reduction of phosphorylation of ERK and JNK, as well as suppression of the NF-κB signaling pathway, were also observed in liver tissues of the alisol F-treated mice model. Alisol F and 25-anhydroalisol F may serve as potential leads for development of anti-inflammatory agents for acute liver failure treatment.
format Online
Article
Text
id pubmed-6152757
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-61527572018-11-13 Anti-Inflammatory Activities and Liver Protection of Alisol F and 25-Anhydroalisol F through the Inhibition of MAPK, STAT3, and NF-κB Activation In Vitro and In Vivo Bi, Xiaoxu Wang, Pu Ma, Qingjuan Han, Li Wang, Xingbo Mu, Yu Guan, Peipei Qu, Xiaodan Wang, Zhanyou Huang, Xueshi Molecules Article Alisol F and 25-anhydroalisol F isolated from Alisma orientale, were proved to exhibit anti-inflammatory potential in our previous work. In the current study, the anti-inflammatory effects and action mechanisms of alisol F and 25-anhydroalisol F were investigated in vitro. Moreover, the pharmacological effects of alisol F in lipopolysaccharide (LPS)/d-galactosamine (d-gal)-induced acute liver-injured mice were evaluated. The results demonstrated that alisol F and 25-anhydroalisol F could suppress LPS-induced production of nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), and interleukin-1β (IL-1β), as well as inhibit the mRNA and protein levels of inducible nitric oxide (iNOS) and cyclooxygenase-2 (COX-2). In addition, we investigated the role of alisol F and 25-anhydroalisol F in mediating mitogen-activated protein kinases (MAPKs), signal transducers, and activators of transcription 3 (STAT3) and nuclear factor κB (NF-κB) pathways involved in the inflammation process of LPS-stimulated RAW 264.7 cells. The phosphorylation of ERK, JNK, p38, and STAT3, and the NF-κB signaling pathway, were obviously suppressed in alisol F and 25-anhydroalisol F treated cells. Results obtained from in vitro experiments suggested alisol F obviously improved liver pathological injury by inhibiting the production of TNF-α, IL-1β, and IL-6, and significantly decreasing the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in LPS/d-gal-induced mice. Furthermore, the reduction of phosphorylation of ERK and JNK, as well as suppression of the NF-κB signaling pathway, were also observed in liver tissues of the alisol F-treated mice model. Alisol F and 25-anhydroalisol F may serve as potential leads for development of anti-inflammatory agents for acute liver failure treatment. MDPI 2017-06-08 /pmc/articles/PMC6152757/ /pubmed/28594379 http://dx.doi.org/10.3390/molecules22060951 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bi, Xiaoxu
Wang, Pu
Ma, Qingjuan
Han, Li
Wang, Xingbo
Mu, Yu
Guan, Peipei
Qu, Xiaodan
Wang, Zhanyou
Huang, Xueshi
Anti-Inflammatory Activities and Liver Protection of Alisol F and 25-Anhydroalisol F through the Inhibition of MAPK, STAT3, and NF-κB Activation In Vitro and In Vivo
title Anti-Inflammatory Activities and Liver Protection of Alisol F and 25-Anhydroalisol F through the Inhibition of MAPK, STAT3, and NF-κB Activation In Vitro and In Vivo
title_full Anti-Inflammatory Activities and Liver Protection of Alisol F and 25-Anhydroalisol F through the Inhibition of MAPK, STAT3, and NF-κB Activation In Vitro and In Vivo
title_fullStr Anti-Inflammatory Activities and Liver Protection of Alisol F and 25-Anhydroalisol F through the Inhibition of MAPK, STAT3, and NF-κB Activation In Vitro and In Vivo
title_full_unstemmed Anti-Inflammatory Activities and Liver Protection of Alisol F and 25-Anhydroalisol F through the Inhibition of MAPK, STAT3, and NF-κB Activation In Vitro and In Vivo
title_short Anti-Inflammatory Activities and Liver Protection of Alisol F and 25-Anhydroalisol F through the Inhibition of MAPK, STAT3, and NF-κB Activation In Vitro and In Vivo
title_sort anti-inflammatory activities and liver protection of alisol f and 25-anhydroalisol f through the inhibition of mapk, stat3, and nf-κb activation in vitro and in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152757/
https://www.ncbi.nlm.nih.gov/pubmed/28594379
http://dx.doi.org/10.3390/molecules22060951
work_keys_str_mv AT bixiaoxu antiinflammatoryactivitiesandliverprotectionofalisolfand25anhydroalisolfthroughtheinhibitionofmapkstat3andnfkbactivationinvitroandinvivo
AT wangpu antiinflammatoryactivitiesandliverprotectionofalisolfand25anhydroalisolfthroughtheinhibitionofmapkstat3andnfkbactivationinvitroandinvivo
AT maqingjuan antiinflammatoryactivitiesandliverprotectionofalisolfand25anhydroalisolfthroughtheinhibitionofmapkstat3andnfkbactivationinvitroandinvivo
AT hanli antiinflammatoryactivitiesandliverprotectionofalisolfand25anhydroalisolfthroughtheinhibitionofmapkstat3andnfkbactivationinvitroandinvivo
AT wangxingbo antiinflammatoryactivitiesandliverprotectionofalisolfand25anhydroalisolfthroughtheinhibitionofmapkstat3andnfkbactivationinvitroandinvivo
AT muyu antiinflammatoryactivitiesandliverprotectionofalisolfand25anhydroalisolfthroughtheinhibitionofmapkstat3andnfkbactivationinvitroandinvivo
AT guanpeipei antiinflammatoryactivitiesandliverprotectionofalisolfand25anhydroalisolfthroughtheinhibitionofmapkstat3andnfkbactivationinvitroandinvivo
AT quxiaodan antiinflammatoryactivitiesandliverprotectionofalisolfand25anhydroalisolfthroughtheinhibitionofmapkstat3andnfkbactivationinvitroandinvivo
AT wangzhanyou antiinflammatoryactivitiesandliverprotectionofalisolfand25anhydroalisolfthroughtheinhibitionofmapkstat3andnfkbactivationinvitroandinvivo
AT huangxueshi antiinflammatoryactivitiesandliverprotectionofalisolfand25anhydroalisolfthroughtheinhibitionofmapkstat3andnfkbactivationinvitroandinvivo