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Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli
Microalgae are recognized as a third generation feedstock for biofuel production due to their rapid growth rates and lignin-free characteristics. In this study, a lipid extracted microalgal biomass residues was used as the raw material to produce isoprene, α-pinene and β-pinene with an engineered E....
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152769/ https://www.ncbi.nlm.nih.gov/pubmed/28598364 http://dx.doi.org/10.3390/molecules22060960 |
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author | Wang, Sumeng Yang, Jianming |
author_facet | Wang, Sumeng Yang, Jianming |
author_sort | Wang, Sumeng |
collection | PubMed |
description | Microalgae are recognized as a third generation feedstock for biofuel production due to their rapid growth rates and lignin-free characteristics. In this study, a lipid extracted microalgal biomass residues was used as the raw material to produce isoprene, α-pinene and β-pinene with an engineered E. coli strain. We adopted an optimal sulfuric acid hydrolysis method (1:7 ratio of solid to acid solution, 32% (w/v) concentration of sulfuric acid solution at 90 °C for 90 min) to efficiently convert holocellulose into glucose efficiently (6.37 g/L). Futhermore, we explored a novel detoxification strategy (phosphoric acid/calcium hydroxide) to remove inhibitors and notably acetic acid, furfural and 5-hydroxymethylfurfural (5-HMF) were reduced by 5.32%, different number given later 99.19% and 98.22%, respectively. Finally, the fermentation concentrations of isoprene (223.23 mg/L), α-pinene (382.21 μg/L) and β-pinene (17.4 mg/L) were achieved using the detoxified hydrolysate as the carbon source, equivalent to approximately 86.02%, 90.16% and 88.32% of those produced by the engineered E. coli strain fermented on pure glucose, respectively. |
format | Online Article Text |
id | pubmed-6152769 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61527692018-11-13 Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli Wang, Sumeng Yang, Jianming Molecules Article Microalgae are recognized as a third generation feedstock for biofuel production due to their rapid growth rates and lignin-free characteristics. In this study, a lipid extracted microalgal biomass residues was used as the raw material to produce isoprene, α-pinene and β-pinene with an engineered E. coli strain. We adopted an optimal sulfuric acid hydrolysis method (1:7 ratio of solid to acid solution, 32% (w/v) concentration of sulfuric acid solution at 90 °C for 90 min) to efficiently convert holocellulose into glucose efficiently (6.37 g/L). Futhermore, we explored a novel detoxification strategy (phosphoric acid/calcium hydroxide) to remove inhibitors and notably acetic acid, furfural and 5-hydroxymethylfurfural (5-HMF) were reduced by 5.32%, different number given later 99.19% and 98.22%, respectively. Finally, the fermentation concentrations of isoprene (223.23 mg/L), α-pinene (382.21 μg/L) and β-pinene (17.4 mg/L) were achieved using the detoxified hydrolysate as the carbon source, equivalent to approximately 86.02%, 90.16% and 88.32% of those produced by the engineered E. coli strain fermented on pure glucose, respectively. MDPI 2017-06-09 /pmc/articles/PMC6152769/ /pubmed/28598364 http://dx.doi.org/10.3390/molecules22060960 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Sumeng Yang, Jianming Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli |
title | Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli |
title_full | Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli |
title_fullStr | Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli |
title_full_unstemmed | Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli |
title_short | Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli |
title_sort | isoprenoids production from lipid-extracted microalgal biomass residues using engineered e. coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152769/ https://www.ncbi.nlm.nih.gov/pubmed/28598364 http://dx.doi.org/10.3390/molecules22060960 |
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