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Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli

Microalgae are recognized as a third generation feedstock for biofuel production due to their rapid growth rates and lignin-free characteristics. In this study, a lipid extracted microalgal biomass residues was used as the raw material to produce isoprene, α-pinene and β-pinene with an engineered E....

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Autores principales: Wang, Sumeng, Yang, Jianming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152769/
https://www.ncbi.nlm.nih.gov/pubmed/28598364
http://dx.doi.org/10.3390/molecules22060960
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author Wang, Sumeng
Yang, Jianming
author_facet Wang, Sumeng
Yang, Jianming
author_sort Wang, Sumeng
collection PubMed
description Microalgae are recognized as a third generation feedstock for biofuel production due to their rapid growth rates and lignin-free characteristics. In this study, a lipid extracted microalgal biomass residues was used as the raw material to produce isoprene, α-pinene and β-pinene with an engineered E. coli strain. We adopted an optimal sulfuric acid hydrolysis method (1:7 ratio of solid to acid solution, 32% (w/v) concentration of sulfuric acid solution at 90 °C for 90 min) to efficiently convert holocellulose into glucose efficiently (6.37 g/L). Futhermore, we explored a novel detoxification strategy (phosphoric acid/calcium hydroxide) to remove inhibitors and notably acetic acid, furfural and 5-hydroxymethylfurfural (5-HMF) were reduced by 5.32%, different number given later 99.19% and 98.22%, respectively. Finally, the fermentation concentrations of isoprene (223.23 mg/L), α-pinene (382.21 μg/L) and β-pinene (17.4 mg/L) were achieved using the detoxified hydrolysate as the carbon source, equivalent to approximately 86.02%, 90.16% and 88.32% of those produced by the engineered E. coli strain fermented on pure glucose, respectively.
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spelling pubmed-61527692018-11-13 Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli Wang, Sumeng Yang, Jianming Molecules Article Microalgae are recognized as a third generation feedstock for biofuel production due to their rapid growth rates and lignin-free characteristics. In this study, a lipid extracted microalgal biomass residues was used as the raw material to produce isoprene, α-pinene and β-pinene with an engineered E. coli strain. We adopted an optimal sulfuric acid hydrolysis method (1:7 ratio of solid to acid solution, 32% (w/v) concentration of sulfuric acid solution at 90 °C for 90 min) to efficiently convert holocellulose into glucose efficiently (6.37 g/L). Futhermore, we explored a novel detoxification strategy (phosphoric acid/calcium hydroxide) to remove inhibitors and notably acetic acid, furfural and 5-hydroxymethylfurfural (5-HMF) were reduced by 5.32%, different number given later 99.19% and 98.22%, respectively. Finally, the fermentation concentrations of isoprene (223.23 mg/L), α-pinene (382.21 μg/L) and β-pinene (17.4 mg/L) were achieved using the detoxified hydrolysate as the carbon source, equivalent to approximately 86.02%, 90.16% and 88.32% of those produced by the engineered E. coli strain fermented on pure glucose, respectively. MDPI 2017-06-09 /pmc/articles/PMC6152769/ /pubmed/28598364 http://dx.doi.org/10.3390/molecules22060960 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Sumeng
Yang, Jianming
Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli
title Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli
title_full Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli
title_fullStr Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli
title_full_unstemmed Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli
title_short Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using Engineered E. coli
title_sort isoprenoids production from lipid-extracted microalgal biomass residues using engineered e. coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152769/
https://www.ncbi.nlm.nih.gov/pubmed/28598364
http://dx.doi.org/10.3390/molecules22060960
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