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Biosynthesis of ZnO Nanoparticles by a New Pichia kudriavzevii Yeast Strain and Evaluation of Their Antimicrobial and Antioxidant Activities

The potential ability of a new yeast strain, Pichia kudriavzevii, in the synthesis of zinc oxide nanoparticles (ZnO-NPs) through a green method was explored in this study. The effect of reaction time (12, 24 and 36 h) on the structure of the resulting ZnO nanoparticles was investigated. From the XRD...

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Autores principales: Boroumand Moghaddam, Amin, Moniri, Mona, Azizi, Susan, Abdul Rahim, Raha, Bin Ariff, Arbakariya, Zuhainis Saad, Wan, Namvar, Farideh, Navaderi, Mohammad, Mohamad, Rosfarizan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152784/
https://www.ncbi.nlm.nih.gov/pubmed/28538674
http://dx.doi.org/10.3390/molecules22060872
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author Boroumand Moghaddam, Amin
Moniri, Mona
Azizi, Susan
Abdul Rahim, Raha
Bin Ariff, Arbakariya
Zuhainis Saad, Wan
Namvar, Farideh
Navaderi, Mohammad
Mohamad, Rosfarizan
author_facet Boroumand Moghaddam, Amin
Moniri, Mona
Azizi, Susan
Abdul Rahim, Raha
Bin Ariff, Arbakariya
Zuhainis Saad, Wan
Namvar, Farideh
Navaderi, Mohammad
Mohamad, Rosfarizan
author_sort Boroumand Moghaddam, Amin
collection PubMed
description The potential ability of a new yeast strain, Pichia kudriavzevii, in the synthesis of zinc oxide nanoparticles (ZnO-NPs) through a green method was explored in this study. The effect of reaction time (12, 24 and 36 h) on the structure of the resulting ZnO nanoparticles was investigated. From the XRD and TEM results, the ZnO-NPs with a hexagonal wurtzite structure and a particle crystal size of ~10–61 nm was formed at different reaction times. Combing XRD, TEM, and PL results, it was revealed that the sample prepared at intermediate duration (24 h) has the most favorable nanosized structure with the lowest defect concentration. The biomedical properties of ZnO-NPs as free radical scavenging activity, cytotoxicity and antibacterial agents were characterized. Biosynthesized ZnO-NPs showed strong DPPH free radical scavenging and a dose dependent toxicity with non-toxic effects on Vero cells for concentrations below 190 µg/mL. Desirable bactericidal activity was shown by the ZnO-NPs on Gram-positive bacteria (Bacillus subtilis, Staphylococcus epidermidis and Staphylococcus aurous) and Gram-negative bacteria (Escherichia coli and Serratia marcescens). A maximum inhibition zone of ~19 mm was observed for Staphylococcus epidermidis at a concentration of 100 µg/mL for sample prepared at 24 h. The results from this study reveal that ZnO-NPs possesses potential for many medical and industrial applications.
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spelling pubmed-61527842018-11-13 Biosynthesis of ZnO Nanoparticles by a New Pichia kudriavzevii Yeast Strain and Evaluation of Their Antimicrobial and Antioxidant Activities Boroumand Moghaddam, Amin Moniri, Mona Azizi, Susan Abdul Rahim, Raha Bin Ariff, Arbakariya Zuhainis Saad, Wan Namvar, Farideh Navaderi, Mohammad Mohamad, Rosfarizan Molecules Article The potential ability of a new yeast strain, Pichia kudriavzevii, in the synthesis of zinc oxide nanoparticles (ZnO-NPs) through a green method was explored in this study. The effect of reaction time (12, 24 and 36 h) on the structure of the resulting ZnO nanoparticles was investigated. From the XRD and TEM results, the ZnO-NPs with a hexagonal wurtzite structure and a particle crystal size of ~10–61 nm was formed at different reaction times. Combing XRD, TEM, and PL results, it was revealed that the sample prepared at intermediate duration (24 h) has the most favorable nanosized structure with the lowest defect concentration. The biomedical properties of ZnO-NPs as free radical scavenging activity, cytotoxicity and antibacterial agents were characterized. Biosynthesized ZnO-NPs showed strong DPPH free radical scavenging and a dose dependent toxicity with non-toxic effects on Vero cells for concentrations below 190 µg/mL. Desirable bactericidal activity was shown by the ZnO-NPs on Gram-positive bacteria (Bacillus subtilis, Staphylococcus epidermidis and Staphylococcus aurous) and Gram-negative bacteria (Escherichia coli and Serratia marcescens). A maximum inhibition zone of ~19 mm was observed for Staphylococcus epidermidis at a concentration of 100 µg/mL for sample prepared at 24 h. The results from this study reveal that ZnO-NPs possesses potential for many medical and industrial applications. MDPI 2017-05-24 /pmc/articles/PMC6152784/ /pubmed/28538674 http://dx.doi.org/10.3390/molecules22060872 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Boroumand Moghaddam, Amin
Moniri, Mona
Azizi, Susan
Abdul Rahim, Raha
Bin Ariff, Arbakariya
Zuhainis Saad, Wan
Namvar, Farideh
Navaderi, Mohammad
Mohamad, Rosfarizan
Biosynthesis of ZnO Nanoparticles by a New Pichia kudriavzevii Yeast Strain and Evaluation of Their Antimicrobial and Antioxidant Activities
title Biosynthesis of ZnO Nanoparticles by a New Pichia kudriavzevii Yeast Strain and Evaluation of Their Antimicrobial and Antioxidant Activities
title_full Biosynthesis of ZnO Nanoparticles by a New Pichia kudriavzevii Yeast Strain and Evaluation of Their Antimicrobial and Antioxidant Activities
title_fullStr Biosynthesis of ZnO Nanoparticles by a New Pichia kudriavzevii Yeast Strain and Evaluation of Their Antimicrobial and Antioxidant Activities
title_full_unstemmed Biosynthesis of ZnO Nanoparticles by a New Pichia kudriavzevii Yeast Strain and Evaluation of Their Antimicrobial and Antioxidant Activities
title_short Biosynthesis of ZnO Nanoparticles by a New Pichia kudriavzevii Yeast Strain and Evaluation of Their Antimicrobial and Antioxidant Activities
title_sort biosynthesis of zno nanoparticles by a new pichia kudriavzevii yeast strain and evaluation of their antimicrobial and antioxidant activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152784/
https://www.ncbi.nlm.nih.gov/pubmed/28538674
http://dx.doi.org/10.3390/molecules22060872
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