Rapid Detection of Rongalite via a Sandwich Lateral Flow Strip Assay Using a Pair of Aptamers

A sandwich lateral flow strip assay (LFSA) using a couple of aptamers functionalized with gold nanoparticles (AuNPs) was designed to assess the presence of rongalite in agrifood products. More specifically, a biotin-labeled primary A09 aptamer immobilized on a streptavidin-coated membrane and a seco...

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Autores principales: Li, Jie, Jing, Le, Song, Yuzhu, Zhang, Jinyang, Chen, Qiang, Wang, Binghui, Xia, Xueshan, Han, Qinqin
Format: Online Artículo Texto
Sprog:English
Udgivet: Springer US 2018
Fag:
Nano Express
Online adgang:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153204/
https://www.ncbi.nlm.nih.gov/pubmed/30251167
http://dx.doi.org/10.1186/s11671-018-2709-9
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author Li, Jie
Jing, Le
Song, Yuzhu
Zhang, Jinyang
Chen, Qiang
Wang, Binghui
Xia, Xueshan
Han, Qinqin
author_facet Li, Jie
Jing, Le
Song, Yuzhu
Zhang, Jinyang
Chen, Qiang
Wang, Binghui
Xia, Xueshan
Han, Qinqin
author_sort Li, Jie
collection PubMed
description A sandwich lateral flow strip assay (LFSA) using a couple of aptamers functionalized with gold nanoparticles (AuNPs) was designed to assess the presence of rongalite in agrifood products. More specifically, a biotin-labeled primary A09 aptamer immobilized on a streptavidin-coated membrane and a secondary B09 aptamer conjugated with AuNPs were developed as capturing and signaling probes, respectively. This system allows the successful and direct detection of rongalite in food samples with concentrations as low as 1 μg/mL, simply by observing the color change of LFSA control and test line.
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spelling pubmed-61532042018-10-09 Rapid Detection of Rongalite via a Sandwich Lateral Flow Strip Assay Using a Pair of Aptamers Li, Jie Jing, Le Song, Yuzhu Zhang, Jinyang Chen, Qiang Wang, Binghui Xia, Xueshan Han, Qinqin Nanoscale Res Lett Nano Express A sandwich lateral flow strip assay (LFSA) using a couple of aptamers functionalized with gold nanoparticles (AuNPs) was designed to assess the presence of rongalite in agrifood products. More specifically, a biotin-labeled primary A09 aptamer immobilized on a streptavidin-coated membrane and a secondary B09 aptamer conjugated with AuNPs were developed as capturing and signaling probes, respectively. This system allows the successful and direct detection of rongalite in food samples with concentrations as low as 1 μg/mL, simply by observing the color change of LFSA control and test line. Springer US 2018-09-24 /pmc/articles/PMC6153204/ /pubmed/30251167 http://dx.doi.org/10.1186/s11671-018-2709-9 Text en © The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Nano Express
Li, Jie
Jing, Le
Song, Yuzhu
Zhang, Jinyang
Chen, Qiang
Wang, Binghui
Xia, Xueshan
Han, Qinqin
Rapid Detection of Rongalite via a Sandwich Lateral Flow Strip Assay Using a Pair of Aptamers
title Rapid Detection of Rongalite via a Sandwich Lateral Flow Strip Assay Using a Pair of Aptamers
title_full Rapid Detection of Rongalite via a Sandwich Lateral Flow Strip Assay Using a Pair of Aptamers
title_fullStr Rapid Detection of Rongalite via a Sandwich Lateral Flow Strip Assay Using a Pair of Aptamers
title_full_unstemmed Rapid Detection of Rongalite via a Sandwich Lateral Flow Strip Assay Using a Pair of Aptamers
title_short Rapid Detection of Rongalite via a Sandwich Lateral Flow Strip Assay Using a Pair of Aptamers
title_sort rapid detection of rongalite via a sandwich lateral flow strip assay using a pair of aptamers
topic Nano Express
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153204/
https://www.ncbi.nlm.nih.gov/pubmed/30251167
http://dx.doi.org/10.1186/s11671-018-2709-9
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