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Bovine Epithelial in vitro Infection Models for Mycoplasma bovis

Mycoplasma bovis causes bovine mycoplasmosis. The major clinical manifestations are pneumonia and mastitis. Recently an increase in the severity of mastitis cases was reported in Switzerland. At the molecular level, there is limited understanding of the mechanisms of pathogenicity of M. bovis. Host–...

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Autores principales: Josi, Christoph, Bürki, Sibylle, Stojiljkovic, Ana, Wellnitz, Olga, Stoffel, Michael H., Pilo, Paola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153342/
https://www.ncbi.nlm.nih.gov/pubmed/30280094
http://dx.doi.org/10.3389/fcimb.2018.00329
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author Josi, Christoph
Bürki, Sibylle
Stojiljkovic, Ana
Wellnitz, Olga
Stoffel, Michael H.
Pilo, Paola
author_facet Josi, Christoph
Bürki, Sibylle
Stojiljkovic, Ana
Wellnitz, Olga
Stoffel, Michael H.
Pilo, Paola
author_sort Josi, Christoph
collection PubMed
description Mycoplasma bovis causes bovine mycoplasmosis. The major clinical manifestations are pneumonia and mastitis. Recently an increase in the severity of mastitis cases was reported in Switzerland. At the molecular level, there is limited understanding of the mechanisms of pathogenicity of M. bovis. Host–pathogen interactions were primarily studied using primary bovine blood cells. Therefore, little is known about the impact of M. bovis on other cell types present in infected tissues. Clear in vitro phenotypes linked to the virulence of M. bovis strains or tissue predilection of specific M. bovis strains have not yet been described. We adapted bovine in vitro systems to investigate infection of epithelial cells with M. bovis using a cell line (MDBK: Madin-Darby bovine kidney cells) and two primary cells (PECT: bovine embryonic turbinate cells and bMec: bovine mammary gland epithelial cells). Two strains isolated before and after the emergence of severe mastitis cases were selected. Strain JF4278 isolated from a cow with mastitis and pneumonia in 2008 and strain L22/93 isolated in 1993 were used to assess the virulence of M. bovis genotypes toward epithelial cells with particular emphasis on mammary gland cells. Our findings indicate that M. bovis is able to adhere to and invade different epithelial cell types. Higher titers of JF4278 than L22/93 were observed in co-cultures with cells. The differences in titers reached between the two strains was more prominent for bMec cells than for MDBK and PECT cells. Moreover, M. bovis strain L22/93 induced apoptosis in MDBK cells and cytotoxicity in PECT cells but not in bMec cells. Dose-dependent variations in proliferation of primary epithelial cells were observed after M. bovis infection. Nevertheless, an indisputable phenotype that could be related to the increased virulence toward mammary gland cells is not obvious.
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spelling pubmed-61533422018-10-02 Bovine Epithelial in vitro Infection Models for Mycoplasma bovis Josi, Christoph Bürki, Sibylle Stojiljkovic, Ana Wellnitz, Olga Stoffel, Michael H. Pilo, Paola Front Cell Infect Microbiol Cellular and Infection Microbiology Mycoplasma bovis causes bovine mycoplasmosis. The major clinical manifestations are pneumonia and mastitis. Recently an increase in the severity of mastitis cases was reported in Switzerland. At the molecular level, there is limited understanding of the mechanisms of pathogenicity of M. bovis. Host–pathogen interactions were primarily studied using primary bovine blood cells. Therefore, little is known about the impact of M. bovis on other cell types present in infected tissues. Clear in vitro phenotypes linked to the virulence of M. bovis strains or tissue predilection of specific M. bovis strains have not yet been described. We adapted bovine in vitro systems to investigate infection of epithelial cells with M. bovis using a cell line (MDBK: Madin-Darby bovine kidney cells) and two primary cells (PECT: bovine embryonic turbinate cells and bMec: bovine mammary gland epithelial cells). Two strains isolated before and after the emergence of severe mastitis cases were selected. Strain JF4278 isolated from a cow with mastitis and pneumonia in 2008 and strain L22/93 isolated in 1993 were used to assess the virulence of M. bovis genotypes toward epithelial cells with particular emphasis on mammary gland cells. Our findings indicate that M. bovis is able to adhere to and invade different epithelial cell types. Higher titers of JF4278 than L22/93 were observed in co-cultures with cells. The differences in titers reached between the two strains was more prominent for bMec cells than for MDBK and PECT cells. Moreover, M. bovis strain L22/93 induced apoptosis in MDBK cells and cytotoxicity in PECT cells but not in bMec cells. Dose-dependent variations in proliferation of primary epithelial cells were observed after M. bovis infection. Nevertheless, an indisputable phenotype that could be related to the increased virulence toward mammary gland cells is not obvious. Frontiers Media S.A. 2018-09-18 /pmc/articles/PMC6153342/ /pubmed/30280094 http://dx.doi.org/10.3389/fcimb.2018.00329 Text en Copyright © 2018 Josi, Bürki, Stojiljkovic, Wellnitz, Stoffel and Pilo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Josi, Christoph
Bürki, Sibylle
Stojiljkovic, Ana
Wellnitz, Olga
Stoffel, Michael H.
Pilo, Paola
Bovine Epithelial in vitro Infection Models for Mycoplasma bovis
title Bovine Epithelial in vitro Infection Models for Mycoplasma bovis
title_full Bovine Epithelial in vitro Infection Models for Mycoplasma bovis
title_fullStr Bovine Epithelial in vitro Infection Models for Mycoplasma bovis
title_full_unstemmed Bovine Epithelial in vitro Infection Models for Mycoplasma bovis
title_short Bovine Epithelial in vitro Infection Models for Mycoplasma bovis
title_sort bovine epithelial in vitro infection models for mycoplasma bovis
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153342/
https://www.ncbi.nlm.nih.gov/pubmed/30280094
http://dx.doi.org/10.3389/fcimb.2018.00329
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